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Literature summary for 4.1.3.3 extracted from
- N-acetylneuraminic acid aldolase of Clostridium perfringens: purification, properties and mechanism of action (1972), Arch. Biochem. Biophys., 151, 234-242.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0% residual activity at 0.01 mM | Clostridium perfringens |  |
| Bromopyruvate | - |
Clostridium perfringens | |
| Cu2+ | 76% residual activity at 1 mM, 91% residual activity at 0.1 mM | Clostridium perfringens | |
| Fe2+ | 38% residual activity at 5 mM | Clostridium perfringens | |
| heavy metal ions | - |
Clostridium perfringens | |
| Hg2+ | 0% residual activity at 1 mM, 9% residual activity at 0.01 mM | Clostridium perfringens | |
| N-ethylmaleimide | - |
Clostridium perfringens | |
| NaBH4 | - |
Clostridium perfringens | |
| p-chloromercuribenzoate | - |
Clostridium perfringens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.75 | - |
N-acetylneuraminic acid | - |
Clostridium perfringens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Clostridium perfringens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
gel filtration | Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium perfringens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-Acetyl-D-mannosamine + pyruvate | - |
Clostridium perfringens | N-Acetylneuraminic acid | - |
r | |
| N-Acetylneuraminic acid | - |
Clostridium perfringens | N-Acetyl-D-mannosamine + pyruvate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 protein components, each enzymatically active | Clostridium perfringens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay at | Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
elimination | Clostridium perfringens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Clostridium perfringens |
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