Cloned (Comment) | Organism |
---|---|
cloning and recombinant expression in Escherichia coli strain BL21(DE3) | Staphylococcus hominis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
N-acetylneuraminate | recombinant enzyme, pH and temperature not specified in the publication | Staphylococcus hominis | |
35.1 | - |
pyruvate | recombinant enzyme, pH and temperature not specified in the publication | Staphylococcus hominis | |
131.7 | - |
N-acetyl-D-mannosamine | recombinant enzyme, pH and temperature not specified in the publication | Staphylococcus hominis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus hominis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Staphylococcus hominis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylneuraminate | - |
Staphylococcus hominis | N-acetyl-D-mannosamine + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | recombinant enzyme | Staphylococcus hominis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
syntesis reaction | Staphylococcus hominis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified recombinant enzyme, 2 h, stable | Staphylococcus hominis |
50 | - |
above, purified recombinant enzyme, rapid decrease of activity | Staphylococcus hominis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
synthesis reaction | Staphylococcus hominis |
8 | - |
cleavage reaction | Staphylococcus hominis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | purified recombinant enzyme, 24 h, 4°C, different buffers, over 70% activity remains | Staphylococcus hominis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
pyruvate | recombinant enzyme, pH and temperature not specified in the publication | Staphylococcus hominis | |
0.08 | - |
N-acetyl-D-mannosamine | recombinant enzyme, pH and temperature not specified in the publication | Staphylococcus hominis | |
1.9 | - |
N-acetylneuraminate | recombinant enzyme, pH and temperature not specified in the publication | Staphylococcus hominis |