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Literature summary for 4.1.3.3 extracted from

  • Zhou, C.; Chen, X.; Feng, J.; Xiao, D.; Wu, Q.; Zhu, D.
    Molecular cloning and characterization of a N-acetylneuraminate lyase gene from Staphylococcus hominis (2013), Chin. J. Biotechnol., 29, 480-489.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning and recombinant expression in Escherichia coli strain BL21(DE3) Staphylococcus hominis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
N-acetylneuraminate recombinant enzyme, pH and temperature not specified in the publication Staphylococcus hominis
35.1
-
pyruvate recombinant enzyme, pH and temperature not specified in the publication Staphylococcus hominis
131.7
-
N-acetyl-D-mannosamine recombinant enzyme, pH and temperature not specified in the publication Staphylococcus hominis

Organism

Organism UniProt Comment Textmining
Staphylococcus hominis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Staphylococcus hominis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetylneuraminate
-
Staphylococcus hominis N-acetyl-D-mannosamine + pyruvate
-
r

Subunits

Subunits Comment Organism
homotetramer recombinant enzyme Staphylococcus hominis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
syntesis reaction Staphylococcus hominis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
purified recombinant enzyme, 2 h, stable Staphylococcus hominis
50
-
above, purified recombinant enzyme, rapid decrease of activity Staphylococcus hominis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
synthesis reaction Staphylococcus hominis
8
-
cleavage reaction Staphylococcus hominis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 purified recombinant enzyme, 24 h, 4°C, different buffers, over 70% activity remains Staphylococcus hominis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.08
-
pyruvate recombinant enzyme, pH and temperature not specified in the publication Staphylococcus hominis
0.08
-
N-acetyl-D-mannosamine recombinant enzyme, pH and temperature not specified in the publication Staphylococcus hominis
1.9
-
N-acetylneuraminate recombinant enzyme, pH and temperature not specified in the publication Staphylococcus hominis