BRENDA - Enzyme Database show
show all sequences of 4.1.3.3

Structure of an Escherichia coli N-acetyl-D-neuraminic acid lyase mutant, E192N, in complex with pyruvate at 1.45 A resolution

Campeotto, I.; Carr, S.B.; Trinh, C.H.; Nelson, A.S.; Berry, A.; Phillips, S.E.; Pearson, A.R.; Acta Crystallogr. Sect. F 65, 1088-1090 (2009)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
mutant enzyme E192N, in complex with pyruvate, sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.2, 200 mM ammonium acetate, 18% PEG 3350
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
E192N
the mutant has a 6fold higher specificity (kcat/Km) for dipropylaminocarbonyl-substituted derivatives than wild type enzyme
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0A6L4
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetylneuraminate
-
701496
Escherichia coli
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
Subunits
Subunits
Commentary
Organism
homotetramer
x-ray crystallography
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
mutant enzyme E192N, in complex with pyruvate, sitting drop vapor diffusion method, using 100 mM Tris-HCl pH 8.2, 200 mM ammonium acetate, 18% PEG 3350
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E192N
the mutant has a 6fold higher specificity (kcat/Km) for dipropylaminocarbonyl-substituted derivatives than wild type enzyme
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetylneuraminate
-
701496
Escherichia coli
N-acetyl-D-mannosamine + pyruvate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
x-ray crystallography
Escherichia coli
Other publictions for EC 4.1.3.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748534
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2
1
1
-
3
1
4
3
-
4
-
1
-
2
-
-
-
-
-
-
-
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2
1
2
-
1
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2
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1
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2
1
1
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3
1
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4
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3
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4
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1
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-
-
-
2
1
2
-
1
-
2
-
1
-
-
-
-
-
3
3
748013
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1
-
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2
-
28
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2
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2
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-
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-
-
1
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1
1
-
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1
2
-
5
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2
1
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1
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1
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1
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1
1
1
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1
2
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-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
749312
Ji
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5
9341
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2
-
1
-
-
-
3
6
-
5
-
2
-
3
-
-
1
-
-
-
-
-
4
1
3
-
1
6
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
3
-
6
-
5
-
2
-
-
-
1
-
-
-
-
4
1
3
-
1
6
2
-
-
-
-
-
-
-
6
6
726551
Daniels
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Haemophilus influenzae
ACS Chem. Biol.
9
1025-1032
2014
-
-
-
1
13
-
-
2
-
-
-
1
-
3
-
-
-
1
-
-
-
-
2
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
13
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
2
1
-
-
-
-
2
2
-
2
2
749150
Garcia-Garcia
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PLoS ONE
9
e96976
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-
-
2
-
3
-
-
13
-
-
2
4
-
5
-
-
2
-
-
-
-
-
22
2
3
-
2
13
2
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
13
-
-
2
4
-
-
-
2
-
-
-
-
22
2
3
-
2
13
2
-
-
-
-
-
-
-
13
13
726631
North
Cloning, expression, purificat ...
Staphylococcus aureus, Staphylococcus aureus MRSA252
Acta Crystallogr. Sect. F
69
306-312
2013
-
-
1
1
-
-
-
-
-
-
-
2
-
5
-
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1
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-
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1
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4
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1
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1
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1
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2
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1
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1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
727035
Huynh
Structural basis for substrate ...
Pasteurella multocida
Biochemistry
52
8570-8579
2013
-
-
1
1
1
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
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-
-
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-
-
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-
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1
-
1
1
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-
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1
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1
-
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1
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
727186
Sun
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Escherichia coli
Biores. Technol.
130
23-29
2013
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
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1
-
-
1
-
-
-
-
-
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-
-
-
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1
-
-
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1
-
-
-
-
-
-
-
-
-
727317
Timms
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Staphylococcus aureus, Staphylococcus aureus NCTC 8325
ChemBioChem
14
474-481
2013
-
-
1
1
10
-
-
5
-
-
1
2
-
4
-
-
1
-
1
-
2
-
2
1
1
-
-
5
2
2
-
-
-
-
-
-
-
1
-
1
10
-
-
-
-
5
-
-
1
2
-
-
-
1
1
-
2
-
2
1
1
-
-
5
2
2
-
-
-
-
-
-
-
-
727332
Zhou
Molecular cloning and characte ...
Staphylococcus hominis
Chin. J. Biotechnol.
29
480-489
2013
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
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2
-
2
-
1
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1
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3
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1
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-
1
1
1
-
2
-
2
-
1
-
-
-
-
-
3
3
728608
Xiao
Progesterone receptor-mediated ...
Mus musculus, Mus musculus B6/129S5-NplGt(IRESBetageo)332Lex/Mmucd
PLoS ONE
8
e65607
2013
-
-
1
-
-
-
-
-
-
-
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-
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4
-
-
-
-
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3
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1
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3
-
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-
-
-
-
-
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-
-
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2
1
1
2
-
-
727656
Garcia Garcia
Characterization of a novel N- ...
Staphylococcus carnosus, Staphylococcus carnosus TM300
J. Agric. Food Chem.
60
7450-7456
2012
-
1
1
-
-
-
-
3
-
-
-
4
-
5
-
-
1
-
-
-
1
-
4
-
2
1
4
3
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
3
-
-
-
4
-
-
-
1
-
-
1
-
4
-
2
1
4
3
1
-
-
-
-
-
-
-
3
3
713843
Sanchez-Carron
Molecular characterization of ...
Lactobacillus plantarum
Appl. Environ. Microbiol.
77
2471-2478
2011
-
-
1
-
-
1
-
3
-
-
2
-
-
3
-
-
1
-
-
-
1
-
1
1
2
-
2
-
1
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
3
-
-
2
-
-
-
-
1
-
-
1
-
1
1
2
-
2
-
1
1
1
-
-
-
-
-
3
3
713852
Gao
Chemoenzymatic synthesis of N- ...
Escherichia coli
Appl. Environ. Microbiol.
77
7080-7083
2011
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
714508
Garcia-Garcia
New stabilized FastPrep-CLEAs ...
Lactobacillus plantarum, Lactobacillus plantarum WFCS1
Biores. Technol.
102
6186-6191
2011
-
1
1
-
-
-
-
4
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
-
-
4
4
701855
Hu
Coupled bioconversion for prep ...
Escherichia coli, Escherichia coli TG1, Sus scrofa
Appl. Microbiol. Biotechnol.
85
1383-1391
2010
-
1
2
-
-
1
-
-
-
-
-
1
-
7
-
-
2
-
-
1
5
-
3
-
1
-
-
-
-
-
-
-
-
-
-
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1
2
-
-
-
1
-
-
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-
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1
-
-
-
2
-
1
5
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
701881
Zhang
An efficient method for N-acet ...
Escherichia coli
Appl. Microbiol. Biotechnol.
86
481-489
2010
-
1
1
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
1
-
1
-
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-
-
-
-
-
-
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-
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1
1
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1
1
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1
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
715921
Campeotto
Structural insights into subst ...
Escherichia coli
J. Mol. Biol.
404
56-69
2010
-
-
1
1
19
-
1
17
-
-
-
-
-
2
-
-
1
-
-
-
-
-
3
-
-
-
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18
-
-
-
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1
-
-
-
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1
-
1
19
-
-
1
1
17
-
-
-
-
-
-
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1
-
-
-
-
3
-
-
-
-
18
-
-
-
-
-
-
-
-
19
19
693709
Chu
Homology modeling and molecula ...
Homo sapiens
J. Mol. Model.
15
323-328
2009
-
-
-
-
-
-
-
-
-
-
1
1
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2
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2
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1
1
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2
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-
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-
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-
-
-
-
-
701496
Campeotto
Structure of an Escherichia co ...
Escherichia coli
Acta Crystallogr. Sect. F
65
1088-1090
2009
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
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1
-
-
-
-
-
1
1
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-
1
1
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-
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1
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702067
Devenish
The quaternary structure of Es ...
Escherichia coli, Escherichia coli W3110 / ATCC 27325
Biochem. Biophys. Res. Commun.
388
107-111
2009
-
-
1
-
18
-
-
11
-
-
1
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
1
11
-
-
-
-
-
-
-
-
-
1
-
-
18
-
-
-
-
11
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
1
11
-
-
-
-
-
1
1
-
-
-
690576
Li
Pasteurella multocida sialic a ...
Escherichia coli K-12, Pasteurella multocida
Appl. Microbiol. Biotechnol.
79
963-970
2008
-
-
2
-
-
-
-
6
-
-
4
2
-
4
-
-
2
-
-
-
-
-
3
-
2
-
-
6
2
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
6
-
-
4
2
-
-
-
2
-
-
-
-
3
-
2
-
-
6
2
2
-
-
-
-
-
-
-
-
674987
Lee
Production of N-acetyl-D-neura ...
Escherichia coli
J. Biotechnol.
129
453-460
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677556
Huang
Disaccharides as sialic acid a ...
Escherichia coli
Angew. Chem. Int. Ed. Engl.
46
2249-2253
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
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Ohta
-
Molecular cloning of the N-ace ...
Escherichia coli
Appl. Microbiol. Biotechnol.
24
386-391
1986
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33314
Uchida
Purification and properties of ...
Escherichia coli
J. Biochem.
96
507-522
1984
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33327
Deijl
Configuration of substrate and ...
Clostridium perfringens
Biochem. Biophys. Res. Commun.
111
668-674
1983
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33329
Kolisis
The protection role of pyruvat ...
Clostridium perfringens
FEBS Lett.
121
280-282
1980
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6
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33315
Nees
Purification and characterizat ...
Clostridium perfringens
Hoppe-Seyler's Z. Physiol. Chem.
357
839-853
1976
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33320
Barnett
The reaction of N-acetylneuram ...
Clostridium perfringens
Biochem. J.
143
487-490
1974
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33316
DeVries
N-acetylneuraminic acid aldola ...
Clostridium perfringens
Arch. Biochem. Biophys.
151
234-242
1972
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9
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33326
Arden
Distribution of neuraminidase ...
Corynebacterium belfanti, Corynebacterium diphtheriae, Corynebacterium pseudotuberculosis, Corynebacterium ulcerans
J. Bacteriol.
112
1206-1212
1972
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33317
Barnett
Studies on N-acetylneuraminic ...
Clostridium perfringens
Biochem. J.
125
275-285
1971
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3
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33328
Schauer
Studies on the substrate speci ...
Clostridium perfringens
Hoppe-Seyler's Z. Physiol. Chem.
352
1073-1080
1971
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10
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33319
Sirbasku
Purification and properties of ...
Bos taurus
Biochim. Biophys. Acta
198
479-482
1970
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4
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33330
Comb
The sialic acids. I. Structure ...
Escherichia coli, Rattus norvegicus
J. Biol. Chem.
235
2529-2537
1960
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