Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli K-12 |
into the vector pET22b+ for expression in Eschericha coli cells | Pasteurella multocida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | pyruvate | - |
Pasteurella multocida | |
2.5 | - |
N-acetylneuraminate | - |
Escherichia coli K-12 | |
4.9 | - |
N-acetylneuraminate | - |
Pasteurella multocida | |
22 | - |
pyruvate | - |
Escherichia coli K-12 | |
180 | - |
N-acetyl-D-mannosamine | - |
Escherichia coli K-12 | |
220 | - |
N-acetyl-D-mannosamine | - |
Pasteurella multocida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
determined by SDS-PAGE | Pasteurella multocida |
33000 | - |
determined by SDS-PAGE | Escherichia coli K-12 |
33400 | - |
calculated | Escherichia coli K-12 |
33700 | - |
molecular mass | Pasteurella multocida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetylneuraminate | Pasteurella multocida | - |
N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | Escherichia coli K-12 | - |
N-acetyl-D-mannosamine + pyruvate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P0A6L4 | - |
- |
Pasteurella multocida | Q9CKB0 | the sequence of the cloned gene differs on six bases, T228C, T303C, C624T, G777A, C786T and C832T | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli K-12 |
using a HisTrap_FF column | Pasteurella multocida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-O-methyl-N-acetyl-D-mannosamine + pyruvate | - |
Pasteurella multocida | 8-O-methyl-N-acetylneuraminate | - |
? | |
N-acetylneuraminate | - |
Pasteurella multocida | N-acetyl-D-mannosamine + pyruvate | - |
r | |
N-acetylneuraminate | - |
Escherichia coli K-12 | N-acetyl-D-mannosamine + pyruvate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
EcNanA | - |
Escherichia coli K-12 |
N-Acetylneuraminate lyase | - |
Pasteurella multocida |
N-Acetylneuraminate lyase | - |
Escherichia coli K-12 |
NanA | - |
Pasteurella multocida |
NanA | - |
Escherichia coli K-12 |
PmNanA | - |
Pasteurella multocida |
Sialic acid aldolase | - |
Pasteurella multocida |
Sialic acid aldolase | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
kinetic assay | Pasteurella multocida |
37 | - |
kinetic assay | Escherichia coli K-12 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
pyruvate | - |
Escherichia coli K-12 | |
1.9 | - |
pyruvate | - |
Pasteurella multocida | |
9 | - |
N-acetyl-D-mannosamine | - |
Escherichia coli K-12 | |
10 | - |
N-acetylneuraminate | - |
Escherichia coli K-12 | |
11 | - |
N-acetyl-D-mannosamine | - |
Pasteurella multocida | |
16 | - |
N-acetylneuraminate | - |
Pasteurella multocida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
kinetic assay | Pasteurella multocida |
7.5 | - |
kinetic assay | Escherichia coli K-12 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Pasteurella multocida |
6 | 9 | - |
Escherichia coli K-12 |