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Literature summary for 4.1.3.3 extracted from

  • Li, Y.; Yu, H.; Cao, H.; Lau, K.; Muthana, S.; Tiwari, V.K.; Son, B.; Chen, X.
    Pasteurella multocida sialic acid aldolase: a promising biocatalyst (2008), Appl. Microbiol. Biotechnol., 79, 963-970.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli K-12
into the vector pET22b+ for expression in Eschericha coli cells Pasteurella multocida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 pyruvate
-
Pasteurella multocida
2.5
-
N-acetylneuraminate
-
Escherichia coli K-12
4.9
-
N-acetylneuraminate
-
Pasteurella multocida
22
-
pyruvate
-
Escherichia coli K-12
180
-
N-acetyl-D-mannosamine
-
Escherichia coli K-12
220
-
N-acetyl-D-mannosamine
-
Pasteurella multocida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
determined by SDS-PAGE Pasteurella multocida
33000
-
determined by SDS-PAGE Escherichia coli K-12
33400
-
calculated Escherichia coli K-12
33700
-
molecular mass Pasteurella multocida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetylneuraminate Pasteurella multocida
-
N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate Escherichia coli K-12
-
N-acetyl-D-mannosamine + pyruvate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12 P0A6L4
-
-
Pasteurella multocida Q9CKB0 the sequence of the cloned gene differs on six bases, T228C, T303C, C624T, G777A, C786T and C832T
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli K-12
using a HisTrap_FF column Pasteurella multocida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-O-methyl-N-acetyl-D-mannosamine + pyruvate
-
Pasteurella multocida 8-O-methyl-N-acetylneuraminate
-
?
N-acetylneuraminate
-
Pasteurella multocida N-acetyl-D-mannosamine + pyruvate
-
r
N-acetylneuraminate
-
Escherichia coli K-12 N-acetyl-D-mannosamine + pyruvate
-
r

Synonyms

Synonyms Comment Organism
EcNanA
-
Escherichia coli K-12
N-Acetylneuraminate lyase
-
Pasteurella multocida
N-Acetylneuraminate lyase
-
Escherichia coli K-12
NanA
-
Pasteurella multocida
NanA
-
Escherichia coli K-12
PmNanA
-
Pasteurella multocida
Sialic acid aldolase
-
Pasteurella multocida
Sialic acid aldolase
-
Escherichia coli K-12

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
kinetic assay Pasteurella multocida
37
-
kinetic assay Escherichia coli K-12

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
pyruvate
-
Escherichia coli K-12
1.9
-
pyruvate
-
Pasteurella multocida
9
-
N-acetyl-D-mannosamine
-
Escherichia coli K-12
10
-
N-acetylneuraminate
-
Escherichia coli K-12
11
-
N-acetyl-D-mannosamine
-
Pasteurella multocida
16
-
N-acetylneuraminate
-
Pasteurella multocida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
kinetic assay Pasteurella multocida
7.5
-
kinetic assay Escherichia coli K-12

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Pasteurella multocida
6 9
-
Escherichia coli K-12