Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
Mn2+ | 20% residual activity at 10 mM | Escherichia coli | |
N-ethylmaleimide | 25-27% residual activity at 1 mM | Escherichia coli | |
p-chloromercuribenzoate | 2-7% residual activity at 1 mM | Escherichia coli | |
Zn2+ | 1% residual activity at 10 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
N-glycolylneuraminic acid | - |
Escherichia coli | |
4.8 | - |
N-acetylneuraminic acid | - |
Escherichia coli | |
7.7 | - |
N-acetyl-D-mannosamine | - |
Escherichia coli | |
8.3 | - |
pyruvate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acetylmannosamine + pyruvate | Escherichia coli | N-acetylneuraminic acid is intermediate for colomic acid biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
colominic acid producing strain | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
N-acetyl-D-mannosamine + pyruvate, 37°C | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris/HCl, pH 8, 25 mM MgCl2, 1 mM PMSF, 20% v/v glycerol, stable under prolonged storage | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acetyl-D-mannosamine + pyruvate | - |
Escherichia coli | N-Acetylneuraminic acid | - |
r | |
N-Acetylmannosamine + pyruvate | N-acetylneuraminic acid is intermediate for colomic acid biosynthesis | Escherichia coli | ? | - |
? | |
N-Acetylneuraminic acid | - |
Escherichia coli | N-Acetyl-D-mannosamine + pyruvate | - |
r | |
N-Glycolylneuraminic acid | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4*33000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in 50 mM Tris/HCl, pH 8, 25 mM MgCl2, 1 mM PMSF stable for at least 24 h | Escherichia coli |
50 | - |
stable for at least 40 min | Escherichia coli |
80 | - |
40% loss of activity after 10 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | elimination and condensation | Escherichia coli |