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Literature summary for 4.1.3.3 extracted from

  • Ferrero, M.A.; Reglero, A.; Fernandez-Lopez, M.; Ordas,.; Rodriguez-Aparicio, L.B.
    N-acetyl-D-neuraminic acid lyase generates the sialic acid for colominic acid biosynthesis in Escherichia coli K1 (1996), Biochem. J., 317, 157-165.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Escherichia coli
Hg2+
-
Escherichia coli
Mn2+ 20% residual activity at 10 mM Escherichia coli
N-ethylmaleimide 25-27% residual activity at 1 mM Escherichia coli
p-chloromercuribenzoate 2-7% residual activity at 1 mM Escherichia coli
Zn2+ 1% residual activity at 10 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
N-glycolylneuraminic acid
-
Escherichia coli
4.8
-
N-acetylneuraminic acid
-
Escherichia coli
7.7
-
N-acetyl-D-mannosamine
-
Escherichia coli
8.3
-
pyruvate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-Acetylmannosamine + pyruvate Escherichia coli N-acetylneuraminic acid is intermediate for colomic acid biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
colominic acid producing strain
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
N-acetyl-D-mannosamine + pyruvate, 37°C Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris/HCl, pH 8, 25 mM MgCl2, 1 mM PMSF, 20% v/v glycerol, stable under prolonged storage Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-mannosamine + pyruvate
-
Escherichia coli N-Acetylneuraminic acid
-
r
N-Acetylmannosamine + pyruvate N-acetylneuraminic acid is intermediate for colomic acid biosynthesis Escherichia coli ?
-
?
N-Acetylneuraminic acid
-
Escherichia coli N-Acetyl-D-mannosamine + pyruvate
-
r
N-Glycolylneuraminic acid
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
tetramer 4*33000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
in 50 mM Tris/HCl, pH 8, 25 mM MgCl2, 1 mM PMSF stable for at least 24 h Escherichia coli
50
-
stable for at least 40 min Escherichia coli
80
-
40% loss of activity after 10 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8 elimination and condensation Escherichia coli