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Literature summary for 4.1.3.3 extracted from

  • Garcia-Garcia, M.I.; Sola-Carvajal, A.; Sanchez-Carron, G.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    New stabilized FastPrep-CLEAs for sialic acid synthesis (2011), Biores. Technol., 102, 6186-6191.
    View publication on PubMed

Application

Application Comment Organism
biotechnology cross-linked enzyme aggregates Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21(DE3)pLys Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9
-
pyruvate cross-linked enzyme aggregates, pH 7.5, 37°C Lactiplantibacillus plantarum
19.9
-
pyruvate soluble enzyme, pH not specified in the publication, temperature not specified in the publication Lactiplantibacillus plantarum
160
-
N-acetyl-D-mannosamine soluble enzyme, pH not specified in the publication, temperature not specified in the publication Lactiplantibacillus plantarum
245
-
N-acetyl-D-mannosamine cross-linked enzyme aggregates, pH 7.5, 37°C Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum WFCS1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-neuraminic acid
-
Lactiplantibacillus plantarum pyruvate + N-acetyl-D-mannosamine
-
r
N-acetyl-D-neuraminic acid
-
Lactiplantibacillus plantarum WFCS1 pyruvate + N-acetyl-D-mannosamine
-
r

Synonyms

Synonyms Comment Organism
N-acetyl-d-neuraminic acid aldolase
-
Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
cross-linked enzyme aggregates, stable at pH 9.0 for 6 hours as the soluble enzyme Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
soluble enzyme Lactiplantibacillus plantarum
7.5
-
cross-linked enzyme aggregates Lactiplantibacillus plantarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0074
-
N-acetyl-D-mannosamine cross-linked enzyme aggregates, pH 7.5, 37°C Lactiplantibacillus plantarum
0.03
-
N-acetyl-D-mannosamine soluble enzyme, pH not specified in the publication, temperature not specified in the publication Lactiplantibacillus plantarum
0.09
-
pyruvate cross-linked enzyme aggregates, pH 7.5, 37°C Lactiplantibacillus plantarum
0.11
-
pyruvate soluble enzyme, pH not specified in the publication, temperature not specified in the publication Lactiplantibacillus plantarum