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Literature summary for 4.1.3.3 extracted from

  • Uchida, Y.; Tsukada, Y.; Sugimori, T.
    Purification and properties of N-acetylneuraminate lyase from Escherichia coli (1984), J. Biochem., 96, 507-522.
    View publication on PubMed

Application

Application Comment Organism
analysis enzymatic determination of sialic acid Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0% residual activity at 0.1 mM, 6% residual activity at 0.01 mM Escherichia coli
Cu2+ 37% residual activity at 1 mM, 89% residual activity at 0.1 mM Escherichia coli
Hg2+ 0% residual activity at 0.1 mM, 22% residual activity at 0.01 mM Escherichia coli
hydrogen peroxide 0% residual activity at 10 mM, 16% residual activity at 1 mM Escherichia coli
Iodine 0% residual activity at 0.01 mM, 45% residual activity at 0.001 mM Escherichia coli
N-bromosuccinimide 0% residual activity at 0.01 mM, 36% residual activity at 0.001 mM Escherichia coli
p-chloromercuribenzoate 0% residual activity at 0.1 mM, 9% residual activity at 0.01 mM Escherichia coli
pyruvate elimination Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
N-acetylneuraminic acid
-
Escherichia coli
4.3
-
N-glycolylneuraminic acid
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
3 * 33000, SDS-PAGE Escherichia coli
98000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Oxidation Stability

Oxidation Stability Organism
sensitive to N-bromosuccinimide, H2O2 and iodine Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
56.8
-
N-acetylneuraminic acid Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information condensation: N-acetylglucosamine, N-acetylgalactosamine, glucosamine and galactosamine cannot replace N-acetylmannosamine. Phosphoenolpyruvate, lactate, beta-hydroxypyruvate and other pyruvate derivatives cannot replace pyruvate Escherichia coli ?
-
?
N-Acetyl-D-mannosamine + pyruvate
-
Escherichia coli N-Acetylneuraminic acid
-
r
N-Acetylneuraminic acid
-
Escherichia coli N-Acetyl-D-mannosamine + pyruvate
-
r
N-Glycolylneuraminic acid
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
trimer 3 * 33000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 75
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
pyruvate protects against heat inactivation Escherichia coli
60
-
stable up to Escherichia coli
80
-
77% loss of activity after 10 min, in presence of 5 mM pyruvate 8% loss of activity after 10 min Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
elimination and condensation Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 at 37°C stable for 24 h Escherichia coli