Application | Comment | Organism |
---|---|---|
analysis | enzymatic determination of sialic acid | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0% residual activity at 0.1 mM, 6% residual activity at 0.01 mM | Escherichia coli | |
Cu2+ | 37% residual activity at 1 mM, 89% residual activity at 0.1 mM | Escherichia coli | |
Hg2+ | 0% residual activity at 0.1 mM, 22% residual activity at 0.01 mM | Escherichia coli | |
hydrogen peroxide | 0% residual activity at 10 mM, 16% residual activity at 1 mM | Escherichia coli | |
Iodine | 0% residual activity at 0.01 mM, 45% residual activity at 0.001 mM | Escherichia coli | |
N-bromosuccinimide | 0% residual activity at 0.01 mM, 36% residual activity at 0.001 mM | Escherichia coli | |
p-chloromercuribenzoate | 0% residual activity at 0.1 mM, 9% residual activity at 0.01 mM | Escherichia coli | |
pyruvate | elimination | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
N-acetylneuraminic acid | - |
Escherichia coli | |
4.3 | - |
N-glycolylneuraminic acid | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
3 * 33000, SDS-PAGE | Escherichia coli |
98000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
sensitive to N-bromosuccinimide, H2O2 and iodine | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
56.8 | - |
N-acetylneuraminic acid | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | condensation: N-acetylglucosamine, N-acetylgalactosamine, glucosamine and galactosamine cannot replace N-acetylmannosamine. Phosphoenolpyruvate, lactate, beta-hydroxypyruvate and other pyruvate derivatives cannot replace pyruvate | Escherichia coli | ? | - |
? | |
N-Acetyl-D-mannosamine + pyruvate | - |
Escherichia coli | N-Acetylneuraminic acid | - |
r | |
N-Acetylneuraminic acid | - |
Escherichia coli | N-Acetyl-D-mannosamine + pyruvate | - |
r | |
N-Glycolylneuraminic acid | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 33000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
pyruvate protects against heat inactivation | Escherichia coli |
60 | - |
stable up to | Escherichia coli |
80 | - |
77% loss of activity after 10 min, in presence of 5 mM pyruvate 8% loss of activity after 10 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
elimination and condensation | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | at 37°C stable for 24 h | Escherichia coli |