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Information on EC 3.1.4.3 - phospholipase C
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3.1.4.3 phospholipase CIUBMB Comments
The bacterial enzyme, which is a zinc protein, also acts on sphingomyelin and phosphatidylinositol; that from seminal plasma does not act on phosphatidylinositol.
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Word Map
- 3.1.4.3
- inositol
- agonist
- phosphatidylinositol
- pkc
- phospholipid
- phosphoinositide
- diacylglycerol
- cyclase
- pertussis
-
phorbol
- camp
- platelet
-
arachidonic
- g-proteins
- 1,4,5-trisphosphate
- 4,5-bisphosphate
-
receptor-mediated
- phospholipases
-
muscarinic
- adenylate
- thapsigargin
- trisphosphate
-
adenylyl
-
phosphatidic
-
protein-coupled
- vibrio
-
blocker
- perfringens
- cereus
- pip2
- carbachol
-
phosphatidylinositol-specific
- nifedipine
-
metabotropic
- neomycin
- parahaemolyticus
-
ca2+-free
-
desensitization
-
thromboxane
- bradykinin
-
fura-2
- enterotoxin
- staurosporine
-
13-acetate
-
agonist-induced
- chelerythrine
-
store-operated
-
ryanodine
-
toxin-sensitive
-
prelabeled
- food industry
- analysis
- drug development
- pharmacology
- medicine
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
alpha-toxin, Beta toxin, Beta-hemolysin, Beta-toxin, broad-range phospholipase C, Cbp, CegC1, cholinespecific glycerophosphodiester phosphodiesterase, Clostridium oedematiens beta- and gamma-toxins, Clostridium welchii alpha-toxin, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alpha-toxin
Beta toxin
-
-
-
-
Beta-hemolysin
-
-
-
-
Beta-toxin
-
-
-
-
Cbp
-
-
-
-
cholinespecific glycerophosphodiester phosphodiesterase
Clostridium oedematiens beta- and gamma-toxins
-
-
-
-
Clostridium welchii alpha-toxin
-
-
-
-
Dot/Icm-injected effector
Gamma-toxin
-
-
-
-
Heat labile-hemolysin
-
-
-
-
heat-labile hemolysin
-
-
-
-
Hemolysin
-
-
-
-
Lecithinase
lecithinase C
-
-
-
-
lipophosphodiesterase C
-
-
-
-
lipophosphodiesterase I
-
-
-
-
MTP40 antigen
-
-
-
-
non-specific phospholipase C
NPC
NPC1
NPC2
NPC3
NPC4
NPC5
NPC6
NPP6
nucleotide pyrophosphatase/phosphodiesterase
PC-PLC
phosphatidase C
-
-
-
-
Phosphatidylcholine cholinephosphohydrolase
-
-
-
-
phosphatidylcholine phospholipase C
phosphatidylcholine-hydrolysing phospholipase C
phosphatidylcholine-hydrolyzing phospholipase C
phosphatidylcholine-specific phospholipase C
phospholipase C
phospholipase C/sphingomyelinase
PLC
PLC1
PLC2
PlcB
plcC
PlcC/CegC1
PLCLM
Rv3487c
SMase
-
-
-
-
sphingomyelinase
-
-
-
-
additional information
alpha-toxin
-
-
-
-
Lecithinase
-
-
-
-
PC-PLC
-
-
-
-
PLC
-
-
-
-
additional information
the enzyme is a member of the plant phospholipase family
additional information
the enzyme is a member of the plant phospholipase family
-
additional information
-
cf. EC 3.1.4.12, the enzyme is the prototype of another phosphatase superfamily
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
-
-
-
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
a phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
mode of action of the plant enzyme family of non-specific phospholipases C, overview
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
hydrolysis of phosphoric ester
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine cholinephosphohydrolase
The bacterial enzyme, which is a zinc protein, also acts on sphingomyelin and phosphatidylinositol; that from seminal plasma does not act on phosphatidylinositol.
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CAS REGISTRY NUMBER
COMMENTARY
9001-86-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycero-3-phosphatide + H2O
1,2-diacylglycerol + phosphate
-
-
-
?
1,2-dibutyryl-sn-glycero-3-phosphocholine + H2O
1,2-dibutyryl-sn-glycerol + phosphocholine
-
-
-
-
?
1,2-dicaproylphosphatidylcholine + H2O
1,2-dicaproylglycerol + phosphorylcholine
-
-
-
?
1,2-diheptanoyl-sn-glycero-3-phosphocholine + H2O
1,2-diheptanoyl-sn-glycerol + phosphocholine
-
-
-
-
?
1,2-dihexanoyl-sn-glycero-3-phospho-L-serine + H2O
1,2-dihexanoyl-sn-glycerol + phosphorylserine
-
-
?
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
1,2-dihexanoyl-sn-glycerol + phosphocholine
-
-
-
-
?
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O
1,2-dihexanoyl-sn-glycerol + phosphorylcholine
-
-
?
1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O
1,2-dihexanoyl-sn-glycerol + phosphoethanolamine
1,2-dilauroylphosphatidylcholine + H2O
1,2-dilaureoylglycerol + phosphorylcholine
-
-
-
?
1,2-dimyristoylphosphatidylcholine + H2O
1,2-dimyristoylglycerol + phosphorylcholine
-
-
-
?
1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) + H2O
1,2-dioleoyl-sn-glycerol + glycerol 1-phosphate
-
about 25% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
?
1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O
1,2-dioleoyl-sn-glycerol + phospho-L-serine
-
60% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
?
1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O
1,2-dioleoyl-sn-glycerol + phosphocholine
-
100% activity
-
-
?
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine + H2O
1,2-dioleoyl-sn-glycerol + phosphoethanolamine
-
about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
?
1,2-dioleoylphosphatidylcholine + H2O
1,2-dioleoylglycerol + phosphorylcholine
-
-
-
?
1,2-dipalmitoylphosphatidylethanolamine + H2O
1,2-dipalmitoylglycerol + phosphorylethanolamine
-
-
-
?
1-arachidonoyl-2-lysophosphatidylcholine + H2O
1-arachidonoyl-sn-glycerol + phosphorylcholine
best substrate
-
-
?
1-arachidoyl-2-lysophosphatidylcholine + H2O
1-arachidoyl-sn-glycerol + phosphorylcholine
poor substrate
-
-
?
1-lauroyl-lysophosphatidylcholine + H2O
1-lauroyl-sn-glycerol + phosphorylcholine
potent substrate
-
-
?
1-linoleoyl-lysophosphatidylcholine + H2O
1-linoleoyl-sn-glycerol + phosphorylcholine
second best substrate
-
-
?
1-myristoyl-lysophosphatidylcholine + H2O
1-myristoyl-sn-glycerol + phosphorylcholine
potent substrate
-
-
?
1-O-(6-(p-methyl red)-amino-hexanoyl)-2-O-(12-(p-methyl red)-amino-dodecanoyl)-sn-glyceryl-N-(3-(5-BODIPY-pentanoyl)-amino-propyl)-N,Ndimethyl-phosphatidylethanolamine + H2O
?
-
fluorogenic analogue of phosphatidylcholine, direct substrate for real-time measurement of enzyme activity
-
-
?
1-oleoyl-lysophosphatidylcholine + H2O
1-oleoyl-sn-glycerol + phosphorylcholine
-
-
-
?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate + choline
the enzyme hydrolyzes 98.4% of mixed micelle 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine in 1 h
-
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
monopalmitoylglycerol + phosphorylcholine
-
-
-
?
1-stearoyl-lysophosphatidylcholine + H2O
1-stearoyl-sn-glycerol + phosphorylcholine
poor substrate
-
-
?
4-nitrophenylphosphorylcholine + H2O
4-nitrophenol + phosphorylcholine
-
-
-
-
?
BODIPY-phosphatidylcholine + H2O
BODIPY-1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
ceramide aminoethylphosphonate
N-acylsphingosine + aminoethylphosphonate
-
-
-
?
ceramide phosphorylethanolamine
N-acylsphingosine + phosphorylethanolamine
-
-
-
?
diheptanoylphosphatidylcholine + H2O
diheptanoylglycerol + phosphorylcholine
-
-
-
?
dihexanoylphosphatidylcholine + H2O
dihexanoylglycerol + phosphorylcholine
-
-
-
?
dimyristoylphosphatidylcholine + H2O
dimyristoylglycerol + phosphorylcholine
-
substrate in mixed micelles with sodium deoxycholate
-
-
?
egg lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
NPP6 is a choline-specific glycerophosphodiester phosphodiesterase. In marked difference to NPP2 (lysoPLD/autotaxin), which equally hydrolyzes lysophosphatidylcholine, 1-oleoyl lysophosphatidylethanolamine, 1-oleoyl lysophosphatidylserine, and porcine liver lysophosphatidylinositol to form lysophosphatidic acid, NPP6 hydrolyzes only lysophosphatidylcholine
-
-
?
glycerophosphorylcholine + H2O
glycerol + phosphorylcholine
enzyme hydrolyzes GPC efficiently, measurement of GDE activity
-
-
?
L-alpha-lysophosphatidylcholine + H2O
?
substrate of EC 3.1.4.3
-
-
?
lysoPAF + H2O
?
lyso platelet activating factor, partially hydrolyzed
-
-
?
membrane form variant surface glycoproteins + H2O
soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
p-nitrophenyl phenylphosphate + H2O
?
pNPPP, classical NPP substrate
-
-
?
p-nitrophenyl phenylphosphate + H2O
p-nitrophenol + phenylphosphate
pNPPP, classical NPP substrate
-
-
?
p-nitrophenyl phosphorylcholine + H2O
?
recombinant NPP6 efficiently hydrolyzes the classical substrate for phospholipase C
-
-
?
p-nitrophenyl phosphorylcholine + H2O
? + p-nitrophenol
pNPPC, classical NPP substrate
-
-
?
p-nitrophenyl phosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
pNPPC, classical NPP substrate, recombinant NPP6 efficiently hydrolyzes the classical substrate for phospholipase C
-
-
?
p-nitrophenylphosphoryl-choline + H2O
p-nitrophenol + phosphorylcholine
-
-
-
-
?
phosphatidic acid + H2O
1,2-diacyl-sn-glycerol + phosphate
-
specific for
-
-
?
phosphatidic acid + H2O
1,2-sn-diacylglycerol + phosphate
-
-
-
?
phosphatidylethanolamine + H2O
1,2-diacyl-sn-glycerol + phosphoethanolamine
-
-
-
-
?
phosphatidylinositol + H2O
inositol + phosphatidate
-
Ca2+-dependent activity
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
sphingosylphosphorylcholine + H2O
?
SPC, measurement of lysoPLC activity of NPP6 toward choline-containing phospholipids
-
-
?
1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O
1,2-dihexanoyl-sn-glycerol + phosphoethanolamine
-
-
?
1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O
1,2-dihexanoyl-sn-glycerol + phosphoethanolamine
-
less efficient substrate
-
-
?
1,2-dioleoylphosphatidylethanolamine + H2O
1,2-dioleoylglycerol + phosphorylethanolamine
-
-
-
?
1,2-dioleoylphosphatidylethanolamine + H2O
1,2-dioleoylglycerol + phosphorylethanolamine
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
1,2-dipalmitoylglycerol + phosphorylcholine
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
1,2-dipalmitoylglycerol + phosphorylcholine
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
1,2-dipalmitoylglycerol + phosphorylcholine
-
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
1,2-dipalmitoylglycerol + phosphorylcholine
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
1,2-dipalmitoylglycerol + phosphorylcholine
-
-
-
?
1,2-dipalmitoylphosphatidylcholine + H2O
1,2-dipalmitoylglycerol + phosphorylcholine
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
1-palmitoyl-sn-glycerol + phosphorylcholine
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + H2O
1-palmitoyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
cardiolipin + H2O
phosphatidylglycerophosphate + 1,2-diacylglycerol
-
-
-
?
cardiolipin + H2O
phosphatidylglycerophosphate + 1,2-diacylglycerol
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
lysophosphatidylcholine + H2O
monoacylglycerol + phosphorylcholine
-
-
-
?
membrane form variant surface glycoproteins + H2O
soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
-
-
?
membrane form variant surface glycoproteins + H2O
soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
-
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenylglycerol + phosphorylcholine
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenylglycerol + phosphorylcholine
-
-
-
?
phosphatidic acid + H2O
?
-
9.04% of the activity with phosphatidylcholine
-
-
?
phosphatidic acid + H2O
?
-
9.04% of the activity with phosphatidylcholine
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
preferred substrate
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
Clostridium welchii
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
substrate from egg yolk
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
hemolytic PLC
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
nonhemolytic PLC
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
phosphatidylcholine and sphingomyelin are cleaved at equal rate
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
substrate from egg yolk
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
the enzyme shows cell-associated phosphatidylcholine-specific phospholipase C activity in vivo
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
the enzyme shows cell-associated phosphatidylcholine-specific phospholipase C activity in vivo
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylethanolamine + H2O
1,2-diacyl-sn-glycerol + ethanolamine phosphate
-
-
-
-
?
phosphatidylethanolamine + H2O
1,2-diacyl-sn-glycerol + ethanolamine phosphate
-
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
-
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
-
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + 1,2-diacylglycerol
-
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + 1,2-diacylglycerol
-
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + 1,2-diacylglycerol
-
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylethanolamine + H2O
phosphorylethanolamine + diacylglycerol
-
-
-
?
phosphatidylglycerol + H2O
1,2-sn-diacylglycerol + phosphatidate
-
-
-
?
phosphatidylglycerol + H2O
1,2-sn-diacylglycerol + phosphatidate
-
-
-
?
phosphatidylinositol + H2O
1,2-sn-diacylglycerol + phosphoinositol
-
-
-
?
phosphatidylinositol + H2O
1,2-sn-diacylglycerol + phosphoinositol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol + H2O
inositol monophosphate + diacylglycerol
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
inositol 4,5-bisphosphate + phosphatidate
-
Ca2+-independent activity
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
inositol 4,5-bisphosphate + phosphatidate
-
-
-
?
phosphatidylinositol 4,5-bisphosphate + H2O
inositol 4,5-bisphosphate + phosphatidate
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylserine + H2O
phosphorylserine + diacylglycerol
-
-
-
?
phosphatidylserine + H2O
phosphorylserine + diacylglycerol
-
-
-
?
phosphatidylserine + H2O
phosphorylserine + diacylglycerol
-
-
-
?
phosphatidylserine + H2O
phosphorylserine + diacylglycerol
-
nonhemolytic PLC
-
-
?
phosphatidylserine + H2O
phosphorylserine + diacylglycerol
-
-
-
?
sphingomyelin + H2O
ceramide + phosphocholine
-
about 70% activity compared to 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
?
sphingomyelin + H2O
ceramide + phosphorylcholine
-
when substrate is presented in a biological membrane, for example in the human red cell membrane or myelin sheath
-
?
sphingomyelin + H2O
ceramide + phosphorylcholine
-
hemolytic PLC
-
-
?
sphingomyelin + H2O
ceramide + phosphorylcholine
-
phosphatidylcholine and sphingomyelin are cleaved at equal rate
-
-
?
sphingomyelin + H2O
N-acylsphingosine + choline phosphate
-
-
-
-
?
additional information
?
-
-
PLCBC also catalyzes the hydrolysis of phosphatidylethanolamine and phosphatidylserine but with lower efficiency
-
-
?
additional information
?
-
-
modeling of active site and reaction mechanism, development of two different reaction mechanism models, overview
-
-
?
additional information
?
-
-
phospholipase C can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes) at pH 4.0-7.5, highest activity at pH 5.0, 37°C. The liposomes are composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and cholesterol in a 1:1:1 molar ratio, membrane fusion is dependent of the lipid composition, overview. The enzyme takes a molten-globule state, with a large fluctuation at acidic pH, mechanism of enzyme-induced membrane fusion
-
-
?
additional information
?
-
enzyme is not haemolytic for human erythrocytes and not involved in multinucleated giant cell formation or induction of apoptotic cell death
-
-
?
additional information
?
-
-
enzyme is not haemolytic for human erythrocytes and not involved in multinucleated giant cell formation or induction of apoptotic cell death
-
-
?
additional information
?
-
-
the release of Ca2+ from the intracellular calcium stores after application of ATP or carbachol is mediated by phospholipase C
-
-
?
additional information
?
-
-
the enzyme is the major virulence factor in the pathogenesis of gas gangrene. Reduction in gangliosides renders cells more susceptible to the membrane f´damage caused by Cp-PLC
-
-
?
additional information
?
-
-
substrate specificity analysis by molecular docking reveals that the enzyme is also active with phosphatidylethanolamine and phosphatidylinositol, and to a lower level with phosphatidylglycerol, overview
-
-
?
additional information
?
-
-
substrate specificity analysis by molecular docking reveals that the enzyme is also active with phosphatidylethanolamine and phosphatidylinositol, and to a lower level with phosphatidylglycerol, overview
-
-
?
additional information
?
-
-
the signaling pathway of human imidazoline receptor antisera-selected protein IRAS in response to I1-imidazoline receptor agonists is coupled with the activation of isoform PC-PLC and its downstream signal transduction molecule ERK
-
-
?
additional information
?
-
-
isoform PC-PLC and reactive oxygen species are involved in chicken blastodisc differentiation to vascular endothelial cells
-
-
?
additional information
?
-
-
isoform PC-PLC plays an important role in regulating CD16 receptor membrane expression, the CD16-mediated cytolytic mechanism and CD16-triggered signal transduction
-
-
?
additional information
?
-
-
isoforms phosphatidylcholine-specific phospholipase C, i.e. PC-PLC, and phosphatidylinositol-specific phospholipase C, i.e. PI-PLC, control apoptosis by jointly regulating Akt phosphorylation, p53 expression, and affecting cell cycle in vascular endothelial cells
-
-
?
additional information
?
-
release of nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives
-
-
?
additional information
?
-
-
release of nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives
-
-
?
additional information
?
-
recombinant NPP6 does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate (pNP-TMP) and bis(p-nitrophenyl) phosphate. NPP6 does not act on sphingomyelin (SM)
-
-
?
additional information
?
-
-
recombinant NPP6 does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate (pNP-TMP) and bis(p-nitrophenyl) phosphate. NPP6 does not act on sphingomyelin (SM)
-
-
?
additional information
?
-
the recombinant enzyme hydrolyzes a broad phospholipid spectrum, including phosphatidylcholine, phosphatidylglycerol, and phosphatidylinositol, broad substrate preference
-
-
?
additional information
?
-
-
the recombinant enzyme hydrolyzes a broad phospholipid spectrum, including phosphatidylcholine, phosphatidylglycerol, and phosphatidylinositol, broad substrate preference
-
-
?
additional information
?
-
the recombinant enzyme hydrolyzes a broad phospholipid spectrum, including phosphatidylcholine, phosphatidylglycerol, and phosphatidylinositol, broad substrate preference
-
-
?
additional information
?
-
-
no activity with phosphatidylinositol
-
-
?
additional information
?
-
-
the enzyme induces membrane fusion
-
-
?
additional information
?
-
release nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids
-
-
?
additional information
?
-
-
phospholipase C hydrolyzes phosphatidylethanolamine and phosphatidylcholine but not other glycerophospholipids
-
-
?
additional information
?
-
-
enzyme activity is necessary for PLC-induced inflammation
-
-
?
additional information
?
-
-
hemolytic fractions from strain ATCC 19660 cause paralysis, death, dermonecrosis, footpad swelling, and vascular permeability in mice. The toxicity is directly associated with enzymatic activity
-
-
?
additional information
?
-
-
homoserine lactone-mediated quorum sensing regulates the expression of PA0026, which encodes PlcB. PlcB is required for directed twitching motility up a gradient of certain kinds of phospholipids
-
-
?
additional information
?
-
-
phospholipase C causes platelet aggregation in a concentration-dependent manner
-
-
?
additional information
?
-
-
phospholipase C plays a role in pathogenesis of infections by Pseudomonas aeruginosa
-
-
?
additional information
?
-
-
the enzyme, when injected intradermally into the skin of sheep, elicits histopathological lesions virtually identical to that seen in naturally occuring fleecerot
-
-
?
additional information
?
-
-
the catalytic activity of enyme induces substrate vesicle fusion without relase of vesicular content. The presence of phosphatidylserine in the vesicle composition does not modify significantly enzyme. Induced liposome aggregation, but completely abolishes fusion. Enzyme is inactive on phospholipids that lack choline head groups
-
-
?
additional information
?
-
-
The sphingomyelinase, but not the phospholipase C activity, is essential for induction of hot-cold hemolysis in human erythrocytes, that contain both phosphatidylcholine and sphingomyelin but also in goat erythrocytes, which lack phosphatidylcholine, however, in horse erythrocytes, with a large proportion of phosphatidylcholine and almost no sphingomyelin, hot-cold hemolysis induced by PlcHR2 is not observed, overview
-
-
?
additional information
?
-
-
PlcHR2 toxin from Pseudomonas aeruginosa is an enzyme with both phospholipase C and sphingomyelinase activities, PlcHR2 hydrolyzes phosphatidylcholine and sphingomyelin at similar rates, but other phospholipids are cleaved at much lower rates, if at all
-
-
?
additional information
?
-
-
activity measurement with substrate on giant unilamellar lipid vesicles. Enzyme binding to the vesicles appears to be a cooperative process and is irreversible, binding analysis using wild-type enzyme and catalytically inactive enzyme mutant T178A. After the initial cluster of bound enzyme is detected, further binding and catalytic activity follow rapidly. The enzyme preferentially binds the more disordered domains, and, in most cases, the catalytic activity causes the disordering of the other domains, at a further stage of lipid hydrolysis, lipid aggregates are formed and vesicles disintegrate, overview
-
-
?
additional information
?
-
-
PlcHR2 is a secreted toxin that exhibits phospholipase C and sphingomyelinase activities. It shows hydrolytic activity of PlcHR2 on bilayers of varying lipid compositions, e.g. on giant unilamellar vesicles composed of sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, and cholesterol
-
-
?
additional information
?
-
-
phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine
-
-
?
additional information
?
-
-
phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine
-
-
?
additional information
?
-
-
enzyme induces cell cycle progression, overexpression of enzyme induces overexpression of cyclin D3
-
?
additional information
?
-
-
enzyme is regulated through redox- and calcium-dependent manners in alveolar macrophage
-
-
?
additional information
?
-
-
low isoform PC-PLC activity is required for mesenchymal stem cell neuronal differentiation, and basic fibroblast growth factor is necessary for maintaining the neuronal differentiation state
-
-
?
additional information
?
-
-
interaction between PC-PLC and cell division cycle 20 homologue, Cdc20, initiating the degradation of PC-PLC by Cdc20-mediated ubiquitin proteasome pathway
-
-
?
additional information
?
-
the enzyme prefers mixed micelle substrates to liposomal substrates. The rate-limiting steps of hydrolysis of mixed micelle 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and emulsified lysophosphatidylcholine are the bulk step and the surface step, respectively
-
-
?
additional information
?
-
-
cPC-PLC may contribute to the parasite evasion against the host immune response
-
-
?
additional information
?
-
-
mPC-PLC may play a role in the intercellular multiplication of Uronema marinum
-
-
?
additional information
?
-
-
no activity with phosphatidylinositol and phosphatidylethanolamine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycero-3-phosphatide + H2O
1,2-diacylglycerol + phosphate
-
-
-
?
membrane form variant surface glycoproteins + H2O
soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
phosphatidylethanolamine + H2O
1,2-diacyl-sn-glycerol + phosphoethanolamine
-
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
membrane form variant surface glycoproteins + H2O
soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
-
-
?
membrane form variant surface glycoproteins + H2O
soluble variant surface glycoprotein + sn-1,2-dimyristoylglycerol
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + choline phosphate
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
preferred substrate
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacyl-sn-glycerol + phosphorylcholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
Clostridium welchii
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-diacylglycerol + phosphorylcholine
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
the enzyme shows cell-associated phosphatidylcholine-specific phospholipase C activity in vivo
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
the enzyme shows cell-associated phosphatidylcholine-specific phospholipase C activity in vivo
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylcholine + H2O
1,2-sn-diacylglycerol + phosphocholine
-
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
-
-
-
?
phosphatidylethanolamine + H2O
1,2-sn-diacylglycerol + phosphoethanolamine
-
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
phosphatidylinositol-bisphosphate + H2O
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
-
-
?
additional information
?
-
-
PLCBC also catalyzes the hydrolysis of phosphatidylethanolamine and phosphatidylserine but with lower efficiency
-
-
?
additional information
?
-
enzyme is not haemolytic for human erythrocytes and not involved in multinucleated giant cell formation or induction of apoptotic cell death
-
-
?
additional information
?
-
-
enzyme is not haemolytic for human erythrocytes and not involved in multinucleated giant cell formation or induction of apoptotic cell death
-
-
?
additional information
?
-
-
the release of Ca2+ from the intracellular calcium stores after application of ATP or carbachol is mediated by phospholipase C
-
-
?
additional information
?
-
-
the enzyme is the major virulence factor in the pathogenesis of gas gangrene. Reduction in gangliosides renders cells more susceptible to the membrane f´damage caused by Cp-PLC
-
-
?
additional information
?
-
-
the signaling pathway of human imidazoline receptor antisera-selected protein IRAS in response to I1-imidazoline receptor agonists is coupled with the activation of isoform PC-PLC and its downstream signal transduction molecule ERK
-
-
?
additional information
?
-
-
isoform PC-PLC and reactive oxygen species are involved in chicken blastodisc differentiation to vascular endothelial cells
-
-
?
additional information
?
-
-
isoform PC-PLC plays an important role in regulating CD16 receptor membrane expression, the CD16-mediated cytolytic mechanism and CD16-triggered signal transduction
-
-
?
additional information
?
-
-
isoforms phosphatidylcholine-specific phospholipase C, i.e. PC-PLC, and phosphatidylinositol-specific phospholipase C, i.e. PI-PLC, control apoptosis by jointly regulating Akt phosphorylation, p53 expression, and affecting cell cycle in vascular endothelial cells
-
-
?
additional information
?
-
release of nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives
-
-
?
additional information
?
-
-
release of nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives
-
-
?
additional information
?
-
-
the enzyme induces membrane fusion
-
-
?
additional information
?
-
release nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
the enzyme plays a key role in the pathogenesis of bacterial infection
-
-
?
additional information
?
-
-
enzyme activity is necessary for PLC-induced inflammation
-
-
?
additional information
?
-
-
hemolytic fractions from strain ATCC 19660 cause paralysis, death, dermonecrosis, footpad swelling, and vascular permeability in mice. The toxicity is directly associated with enzymatic activity
-
-
?
additional information
?
-
-
homoserine lactone-mediated quorum sensing regulates the expression of PA0026, which encodes PlcB. PlcB is required for directed twitching motility up a gradient of certain kinds of phospholipids
-
-
?
additional information
?
-
-
phospholipase C causes platelet aggregation in a concentration-dependent manner
-
-
?
additional information
?
-
-
phospholipase C plays a role in pathogenesis of infections by Pseudomonas aeruginosa
-
-
?
additional information
?
-
-
the enzyme, when injected intradermally into the skin of sheep, elicits histopathological lesions virtually identical to that seen in naturally occuring fleecerot
-
-
?
additional information
?
-
-
The sphingomyelinase, but not the phospholipase C activity, is essential for induction of hot-cold hemolysis in human erythrocytes, that contain both phosphatidylcholine and sphingomyelin but also in goat erythrocytes, which lack phosphatidylcholine, however, in horse erythrocytes, with a large proportion of phosphatidylcholine and almost no sphingomyelin, hot-cold hemolysis induced by PlcHR2 is not observed, overview
-
-
?
additional information
?
-
-
PlcHR2 is a secreted toxin that exhibits phospholipase C and sphingomyelinase activities. It shows hydrolytic activity of PlcHR2 on bilayers of varying lipid compositions, e.g. on giant unilamellar vesicles composed of sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, and cholesterol
-
-
?
additional information
?
-
-
enzyme induces cell cycle progression, overexpression of enzyme induces overexpression of cyclin D3
-
?
additional information
?
-
-
enzyme is regulated through redox- and calcium-dependent manners in alveolar macrophage
-
-
?
additional information
?
-
-
low isoform PC-PLC activity is required for mesenchymal stem cell neuronal differentiation, and basic fibroblast growth factor is necessary for maintaining the neuronal differentiation state
-
-
?
additional information
?
-
-
interaction between PC-PLC and cell division cycle 20 homologue, Cdc20, initiating the degradation of PC-PLC by Cdc20-mediated ubiquitin proteasome pathway
-
-
?
additional information
?
-
-
cPC-PLC may contribute to the parasite evasion against the host immune response
-
-
?
additional information
?
-
-
mPC-PLC may play a role in the intercellular multiplication of Uronema marinum
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Co2+
Mg2+
Mn2+
Zn2+
additional information
Ca2+
-
recombinant PLC d3: maximum activity with 0.001 mM, recombinant PLC d1: maximum activity with 0.01 mM
Ca2+
-
PLC-beta 1a and -beta 1b: maximum activity with 3 mM with phosphatidylinositol for substrate, PLC translocation to membranes by free Ca2+ concentration of 100 nM
Co2+
-
restores activity after o-phenanthroline-treatment
Mg2+
-
restores ability of Ca2+-inhibited PLC to hydrolyse sphingomyelin
Mn2+
-
restores activity after o-phenanthroline-treatment
Zn2+
-
phospholipase C is a trinuclear zinc enzyme, structure analysis, using also the crystal structure from PDB entry 1AH7, overview
Zn2+
-
zinc-dependent enzyme. His216 is required for coordinate binding of zinc
Zn2+
-
restores activity after o-phenanthroline-treatment
additional information
none of the divalent cations tested (Ca2+, Mg2+, Zn2+, Ni2+, Co2+, Fe2+, and Cu2+) rescued the lost activity after treatment with EDTA, EGTA
additional information
-
none of the divalent cations tested (Ca2+, Mg2+, Zn2+, Ni2+, Co2+, Fe2+, and Cu2+) rescued the lost activity after treatment with EDTA, EGTA
additional information
-
enzyme belongs to the group of metallophospholipases C
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-[6-[[(17beta)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione
-
i.e. U-73122, a phospholipase C inhibitor
1-[hydroxy[2-(trimethylammonio)ethyl]amino]-1-oxododecan-2-aminium
-
uncompetitive inhibition
1-[hydroxy[3-(trimethylammonio)propyl]amino]-1-oxododecan-2-aminium
-
mixed type inhibition
2-[(benzyloxy)[2-[(tert-butoxycarbonyl)amino]dodecanoyl]amino]-N,N,N-trimethylethanaminium
-
-
2-[(benzyloxy)[2-[(tert-butoxycarbonyl)amino]hexadecanoyl]amino]-N,N,N-trimethylethanaminium
-
-
2-[(benzyloxy)[2-[(tert-butoxycarbonyl)amino]icosanoyl]amino]-N,N,N-trimethylethanaminium
-
-
2-[(benzyloxy)[2-[(tert-butoxycarbonyl)amino]octanoyl]amino]-N,N,N-trimethylethanaminium
-
-
3(S),4-dihexanoylbutyl-1-phosphonycholine
-
non-hydrolyzable, competitive inhibitor of bacterial PLC, analog of the natural phospholipid substrate, binds with its phosphonyl group to the three Zn-ions in the active site of the enzyme
3-[(benzyloxy)[2-[(tert-butoxycarbonyl)amino]dodecanoyl]amino]-N,N,N-trimethylpropan-1-aminium
-
mixed type inhibition
3-[(benzyloxy)[2-[(tert-butoxycarbonyl)amino]octanoyl]amino]-N,N,N-trimethylpropan-1-aminium
-
-
48/80
-
compound 48/80, oligomeric mixture of condensation products of N-methyl-p-methoxyphenethylamine and formaldehyde. Specific inhibition of phosphatidylinositol-specific phospholipase C, i.e. PI-PLC. Suppression of PI-PLC promotes apoptosis of vascular endothelial cells by inhibiting Akt phosphorylation, elevating p53 expression, and affecting cell cycle distribution
AlCl3
-
at 1 mM, about 60% inhibition of soluble isoform, 50% inhibition of membrane-associated isoform, inhibition of root growth
EGTA
enzyme activity against 1-myristoyl-lysophosphatidylcholine, glycerophosphorylcholine, and p-nitrophenyl phosphorylcholine are readily inhibited by EGTA. Indicates that a divalent cation is essential for catalytic activity
tert-butyl (1-[(benzyloxy)[3-(dimethylamino)propyl]amino]-1-oxododecan-2-yl)carbamate
-
noncompetitive inhibition
tert-butyl (1-[(benzyloxy)[3-(dimethylamino)propyl]amino]-1-oxooctan-2-yl)carbamate
-
-
tricyclodecan-9-yl-potassium xanthate
-
The recovery of both variables to their original levels in serum-restimulated (or lysophosphatidic acid-restimulated) EOC cells is strongly delayed, for at least 24 h, in the presence of the PC-PLC inhibitor tricyclodecan-9-yl-potassium xanthate (D609). The S-phase of serum-restimulated EONT cells is not sensitive to D609
Al3+
-
Al3+-mediated plasma membrane rigidity decreases the enzyme activity
Ca2+
-
inhibits phosphatidylethanolamine hydrolysis
Ca2+
-
activity rapidly decreases at concentrations of 0.015-0.030 mM and above
Cu2+
-
study of in vivo inhibitory capacity, time course, recovery after 7 days
D609
-
i.e. tricyclodecan-9-yl potassium xanthate, inhibition of isoform PC-PLC. After application on blastodisc at 24 h, cell differentiation cannot progress and intracellular level of reactive oxygen species is elevated
D609
-
specific inhibition of phosphatidylcholine-specific phospholipase C, i.e. PC-PLC. Suppression of PC-PLC promotes the phosphorylation of Akt protein
D609
-
inhibitor of isoform PC-PLC, preincubation with D609 results in a dramatic decrease both in CD16 receptor and PC-PLC expression on the plasma membrane and reduction of CD16-mediated cytotoxicity
D609
-
suppression of isoform PC-PLC by D609 inhibits apoptosis in young cells, but not in senescent cells, and depresses reactive oxygen species levels in both young and senescent cells
D609
-
inhibition of isoform PC-PLC blocks the silica-stimulated induction of TNF-alpha and interleukin-1beta
D609
-
inhibition of isoform PC-PLC activity coupled with induction of neuronal differentiation. Simultaneously, the level of reactive oxygen species, and the activity of NADPH oxidase increase significantly, without alteration of the levels of Mn-superoxide dismutase and Cu/ZN superoxide dismutase
EDTA
enzyme activity against 1-myristoyl-lysophosphatidylcholine, glycerophosphorylcholine, and p-nitrophenyl phosphorylcholine are readily inhibited by EDTA. Indicates that a divalent cation is essential for catalytic activity
Hg2+
-
study of in vivo inhibitory capacity, time course, recovery after 7 days
Mg2+
-
inhibits phosphatidylethanolamine hydrolysis
Mn2+
-
inhibits phosphatidylethanolamine hydrolysis
o-phenanthroline
-
inhibits the phospholipase C hydrolytic activity of the enzyme, but not its membrane fusion activity
tricyclodecan-9-yl-xanthogenate
-
inhibition is associated with decrease of enzyme exposed on cell surface and accumulation of cytoplasmic enzyme in Golgi region; preincubation of NK cells with a PC-PLC inhibitor, tricyclodecan-9-yl-xanthogenate, strongly reduced NK-mediated cytotoxicity
-
tricyclodecan-9-yl-xanthogenate
-
i.e. D609, a specific inhibitor, significantly suppresses the (S)-3-methyl-2-phenyl-N-(1-phenylpropyl)-4-quinolinecarboxamide-induced current in type I nNOS neurons
-
tricyclodecan-9-yl-xanthogenate
-
i.e. D609, specific inhibitor, does not inhibit sphingomyelin synthase
-
tricyclodecan-9-yl-xanthogenate
-
i.e. D609, a specific inhibitor, prevents the development of acute hypoxic pulmonary hypertension in rats, and also inhibits pulmonary vasoconstriction induced with a generator of superoxide anions LY83583, but not the one induced with hydrogen peroxide
-
tricyclodecan-9-ylxanthogenate
i.e. D609; i.e. D609; i.e. D609; i.e. D609; i.e. D609; i.e. D609
tricyclodecan-9-ylxanthogenate
-
i.e. D609, a rather selective PC-PLC inhibitor
U-73122
-
inhibition of enzyme in single myocytes by 0.005 mM completely blocks rises in Ca2+ concentration induced by both carbachol and ATP
U-73122
general PLC antagonist, abolishes all PLC activities of eggs, blocks fertilization-induced egg activation; PLC antagonist, abolishes all PLC activities of eggs ,blocks fertilization-induced egg activation
additional information
-
PC-PLCBc inhibition is not due to the interaction of compounds with the phospholipase catalytic zinc atoms, but rather results from the inhibitor cationic group recognition by the PCPLCBc amino acids involved in choline lipid binding. Inhibitor design based on 2-aminohydroxamic acid PC-PLCBc inhibitors that block the enzyme by coordination of the zinc active site atoms present in PC-PLCBc, overview
-
additional information
-
excess diacylglycerol at pH 7.5 at approximately 12% inhibits the membrane fusion activity of the enzyme
-
additional information
U-73343, has no effect on egg activation; U-73343, has no effect on egg activation
-
additional information
U-73343, has no effect on egg activation; U-73343, has no effect on egg activation
-
additional information
-
no effect on activity by monosialic ganglioside GM3 and phospholipids
-
additional information
-
inhibition of enzyme leads to inhibition of IL-2 induced proliferation of cells
-
additional information
the catalytic activity of NPP6 toward p-nitrophenyl phosphorylcholine is partially inhibited by the divalent cations. Attempts are unsuccessful to recover the activity of EDTA treated NPP6 by adding various divalent cations
-
additional information
-
the catalytic activity of NPP6 toward p-nitrophenyl phosphorylcholine is partially inhibited by the divalent cations. Attempts are unsuccessful to recover the activity of EDTA treated NPP6 by adding various divalent cations
-
additional information
-
no inhibition by tricyclodecan-9-ylxanthogenate, i.e. D609
-
additional information
-
the recombinant enzyme is resistant to 20 mM CHAPS or 20 mM Triton X-100
-
additional information
-
no inhibition of enzymatic activity by Ca2+, inhibits haemolytic activity of the enzyme
-
additional information
-
inhibition of phosphatidylcholine-specific phospholipase C prevents the inhibition of gap junctional intercellular communication by 1-methylanthracene
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cholesterol
-
phospholipase C is enhanced by cholesterol and by lipids with an intrinsic negative curvature, e.g. phosphatidylethanolamine
guanine nucleotide-binding regulatory protein Gh
-
stimulating only in presence of GTPgammaS, lowers required Ca2+ concentration
-
guanine nucleotide-binding regulatory protein Ghalpha
-
stimulation in presence of aluminium fluoride, lowers required Ca2+ concentration
-
moxonidine
-
incubation of CHO cells stably expressing human imidazoline receptor antisera-selected protein IRAS for 5 min can induce an upregulation of isoform PC-PLC activity and an increase in the accumulation of reaction product diacylglycerol
phosphatidylethanolamine
-
phospholipase C is enhanced by cholesterol and by lipids with an intrinsic negative curvature, e.g. phosphatidylethanolamine
rilmenidine
-
incubation of CHO cells stably expressing human imidazoline receptor antisera-selected protein IRAS for 5 min can induce an upregulation of isoform PC-PLC activity and an increase in the accumulation of reaction product diacylglycerol
silica particle
-
enzyme activity in macrophage increases significantly upon exposure to silica particles. Increase in activity is inhibited by Mn(III)tetrakis(4-benzoic acid)porphyrin chloride
-
guanine nucleotide-binding protein G16alpha
-
subunit of cell surface receptor associated G-protein, PLC-beta 1, -beta 2 and -beta 3, half-maximal concentration needed for activation is 1nM, stimulating only in presence of GTP gamma S
-
guanine nucleotide-binding protein G16alpha
-
subunit of cell surface receptor associated G-protein, PLC-beta 1, -beta 2 and -beta 3, half-maximal concentration needed for activation is 1nM, stimulating only in presence of GTP gamma S
-
sodium deoxycholate
-
at concentrations up to 0.83mg/ml, optimal molar ratio of sodium deoxycholate to lecithin: 0.5
subunit of cell surface receptor associated G-protein
-
Gqa, subunit of cell surface receptor associated G-protein, PLC d1
-
subunit of cell surface receptor associated G-protein
-
Gqa, subunit of cell surface receptor associated G-protein, PLC d1
-
Triton X-100
in the presence of 0.05-0.5% and 0.1-0.2% (wt/vol) Triton X-100, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and choline plasmalogen are efficiently hydrolyzed, respectively. Hydrolysis of lysophosphatidylcholine and choline lysoplasmalogen does not require Triton X-100, the hydrolytic activity is inhibited by more than 0.05% (wt/vol) Triton X-100
additional information
-
no effect on activity by monosialic ganglioside GM3 and phospholipids
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.271
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
pH 5.0, 37°C
additional information
additional information
-
kinetic parameters depend on the physicochemical properties of substrate aggregates. Larger micelles with a ratio bile salt/lipid smaller than 5 show higher activity and shorter steady state duration of less than 4 min, smaller micelles with a ratio bile salt/lipid greater than 5 show lower activity and longer steady state of about 10 min
-
14.8
4-nitrophenylphosphorylcholine
-
mutant enzyme F66W, at pH 7.0 and 50°C
17.4
4-nitrophenylphosphorylcholine
-
mutant enzyme F66Y, at pH 7.0 and 50°C