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Literature summary for 3.1.4.3 extracted from
- Phospholipase C from Clostridium perfringens: Preparation and characterization of homogenous enzyme (1984), Arch. Biochem. Biophys., 231, 400-410.
General Stability
| General Stability | Organism |
|---|---|
| 1,10-phenanthroline, denaturation | Clostridium perfringens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Clostridium perfringens |  |
| o-phenanthroline | - |
Clostridium perfringens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Clostridium perfringens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | restores activity after EDTA treatment | Clostridium perfringens | |
| Co2+ | restores activity after EDTA treatment | Clostridium perfringens | |
| Fe3+ | - |
Clostridium perfringens | |
| Zn2+ | restores activity after EDTA treatment | Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Clostridium perfringens |
| 47000 | - |
gel filtration | Clostridium perfringens |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| ethyl alcohol | - |
Clostridium perfringens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, ion-exchange chromatography, affinity chromatography, greater than 99% pure, 94fold | Clostridium perfringens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2100 | - |
substrate: phosphatidylcholine | Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | - |
Clostridium perfringens | ceramide + phosphorylcholine | - |
? | |
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Release 2023.1 (January 2023)
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