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Literature summary for 3.1.4.3 extracted from
- Phospholipase C from Clostridium perfringens (1981), Methods Enzymol., 71, 710-725.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium cholate | - |
Clostridium perfringens |  |
| sodium dehydrocholate | - |
Clostridium perfringens | |
General Stability
| General Stability | Organism |
|---|---|
| 1,10-phenanthroline, denaturation | Clostridium perfringens |
| sodium dodecyl sulfate | Clostridium perfringens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | - |
Clostridium perfringens | |
| EDTA | - |
Clostridium perfringens | |
| ferricyanide | - |
Clostridium perfringens | |
| fluoride | - |
Clostridium perfringens | |
| glutathione | - |
Clostridium perfringens | |
| o-phenanthroline | - |
Clostridium perfringens | |
| phosphinate analogue of lecithin | competitive inhibitor | Clostridium perfringens | |
| phosphonate analogue of lecithin | competitive inhibitor | Clostridium perfringens | |
| putrescine | - |
Clostridium perfringens | |
| SDS | - |
Clostridium perfringens | |
| spermidine | - |
Clostridium perfringens | |
| spermine | - |
Clostridium perfringens | |
| thioglycolic acid | - |
Clostridium perfringens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | 1.2 | lecithin | - |
Clostridium perfringens | |
| 200 | - |
p-Nitrophenylphosphorylcholine | - |
Clostridium perfringens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Clostridium perfringens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Clostridium perfringens | |
| Co2+ | - |
Clostridium perfringens | |
| Mg2+ | - |
Clostridium perfringens | |
| Mn2+ | - |
Clostridium perfringens | |
| Zn2+ | - |
Clostridium perfringens | |
| Zn2+ | metalloenzyme | Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Clostridium perfringens |
| 43000 | - |
SDS-PAGE | Clostridium perfringens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-diacyl-sn-glycero-3-phosphatide + H2O | Clostridium perfringens | - |
1,2-diacylglycerol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
| Clostridium perfringens PB6K | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography, gel filtration, 2500fold purification from culture filtrate | Clostridium perfringens |
| column chromatography, gel filtration | Clostridium perfringens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1620 | - |
substrate: lecithin | Clostridium perfringens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 70 mg crude enzyme/ml, stable for at least a year | Clostridium perfringens |
| -20°C, more than 1-2 mg purified enzyme/ml, stable for at least 3 months | Clostridium perfringens |
| -20°C, stable for more than 6 months, crude enzyme | Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-diacyl-sn-glycero-3-phosphatide + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphate | - |
? | |
| ceramide aminoethylphosphonate | - |
Clostridium perfringens | N-acylsphingosine + aminoethylphosphonate | - |
? | |
| ceramide phosphorylethanolamine | - |
Clostridium perfringens | N-acylsphingosine + phosphorylethanolamine | - |
? | |
| choline plasmalogen | - |
Clostridium perfringens | ? | - |
? | |
| lysophosphatidylcholine + H2O | - |
Clostridium perfringens | monoacylglycerol + phosphorylcholine | - |
? | |
| lysophosphatidylcholine + H2O | - |
Clostridium perfringens PB6K | monoacylglycerol + phosphorylcholine | - |
? | |
| p-nitrophenylphosphoryl-choline + H2O | - |
Clostridium perfringens | p-nitrophenol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylcholine + H2O | - |
Clostridium perfringens PB6K | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylethanolamine + H2O | - |
Clostridium perfringens | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylethanolamine + H2O | - |
Clostridium perfringens PB6K | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylserine + H2O | - |
Clostridium perfringens | phosphorylserine + diacylglycerol | - |
? | |
| phosphatidylserine + H2O | - |
Clostridium perfringens PB6K | phosphorylserine + diacylglycerol | - |
? | |
| sphingomyelin + H2O | - |
Clostridium perfringens | ceramide + phosphorylcholine | - |
? | |
| sphingomyelin + H2O | - |
Clostridium perfringens PB6K | ceramide + phosphorylcholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 31000-43000, gel filtration, SDS-PAGE | Clostridium perfringens |
| polymer | identical subunits of 31000-43000 Da, gel filtration, SDS-PAGE, multiple forms | Clostridium perfringens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
for 60 min, pH 5.0-10.0 | Clostridium perfringens |
| 60 | 70 | deactivation | Clostridium perfringens |
| 100 | - |
almost complete restoration of activity of enzyme deactivated at 60-70°C | Clostridium perfringens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | substrate: phosphatidylcholine | Clostridium perfringens |
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