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Literature summary for 3.1.4.3 extracted from
- Phospholipase C isoforms d1 and d3 from human fibroblasts (1997), Protein Expr. Purif., 9, 262-278.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| tranformed in Escherichia coli | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens |  |
| Ca2+ | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85100 | - |
PLC delta 3, gel electrophoresis | Homo sapiens |
| 85500 | - |
PLC delta 1, gel electrophoresis | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol-bisphosphate + H2O | Homo sapiens | - |
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
| phosphatidylinositol-bisphosphate + H2O | Rattus norvegicus | - |
inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant PLC d1 and D3 with poly-His-tail at the amino terminus, metal ion affinity chromatography | Homo sapiens |
| recombinant PLC d1 and D3 without poly-His-tail at the amino terminus, cation exchange HPLC and phosphocellulose chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 404 | - |
substrate: phosphatidylinositol-4,5-bisphosphate, PLC d3 with poly-His-tail, no significant change of activity after cleavage of poly-His-tail, expression at 16°C for 72 h | Homo sapiens |
| 1268 | - |
substrate: phosphatidylinositol-4,5-bisphosphate, PLC d1 with poly-His-tail, no significant change of activity after cleavage of poly-His-tail, expression at 16°C for 72 h | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, buffer B, purified recombinant PLC d1 and d3 with poly-His-tail, activity stable over 2 years, more than 15 freeze/thaw cycles between -20°C and 4°C reduce activity by 10-20% | Homo sapiens |
| -20°C, crude extract, enzyme activity stable over 2 years | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol-bisphosphate + H2O | - |
Homo sapiens | inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
| phosphatidylinositol-bisphosphate + H2O | - |
Rattus norvegicus | inositol 1,4,5-trisphosphate + 1,2-diacylglycerol | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
for 60 min, recombinant PLC delta 1 and delta 3 with poly-His-tail, no loss of activity | Homo sapiens |
| 37 | - |
for 30 min, recombinant PLC delta 1 and delta 3 with poly-His-tail, no loss of activity | Homo sapiens |
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Release 2023.1 (January 2023)
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