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Literature summary for 3.1.4.3 extracted from

  • Sugimori, D.; Nakamura, M.
    Partial purification and some properties of a phospholipase C from Pseudomonas sp. strain KS3.2 (2006), Biosci. Biotechnol. Biochem., 70, 535-537.
    View publication on PubMed
Search for more literature for 3.1.4.3

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 2 mM, 40% inhibition Pseudomonas sp.
CoCl2 2 mM, 90% inhibition Pseudomonas sp.
CuSO3 2 mM, 96% inhibition Pseudomonas sp.
dithiothreitol 2 mM, 94% inhibition Pseudomonas sp.
EDTA 2 mM, 92% inhibition Pseudomonas sp.
FeCl2 2 mM, 94% inhibition Pseudomonas sp.
FeCl3 2 mM, 90% inhibition Pseudomonas sp.
HgCl2 2 mM, 92% inhibition Pseudomonas sp.
MnCl2 2 mM, 92% inhibition Pseudomonas sp.
phenylmethylsulfonyl fluoride 2 mM, 94% inhibition Pseudomonas sp.
SDS 2 mM, 90% inhibition Pseudomonas sp.
ZnCl2 2 mM, 89% inhibition Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme belongs to the group of metallophospholipases C Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KS3.2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine Pseudomonas sp. ?
-
 ?
additional information phosphatidylethanolamine, sphingomyelin, phosphatidylinositol and phosphatidylserine are hydrolyzed at less than 1% of the activity with phosphatidylcholine Pseudomonas sp. KS3.2 ?
-
 ?
phosphatidic acid + H2O 9.04% of the activity with phosphatidylcholine Pseudomonas sp. ?
-
 ?
phosphatidic acid + H2O 9.04% of the activity with phosphatidylcholine Pseudomonas sp. KS3.2 ?
-
 ?
phosphatidylcholine + H2O
-
 Pseudomonas sp. 1,2-diacylglycerol + phosphorylcholine
-
 ?
phosphatidylcholine + H2O
-
 Pseudomonas sp. KS3.2 1,2-diacylglycerol + phosphorylcholine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 35°C: about 50% of maximal activity, 60°C: about 50% of maximal activity Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 pH 7.2, 30 min, 50% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 50% of maximal activity Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 5°C, 3 h, about 55% loss of activity Pseudomonas sp.
6
-
 5°C, 3 h, about 70% loss of activity Pseudomonas sp.
7
-
 5°C, 3 h, about 35% loss of activity Pseudomonas sp.
8 9 5°C, 3 h, stable Pseudomonas sp.