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Literature summary for 3.1.4.3 extracted from
- Studies on phospholipases from Streptomyces. Purification and properties of Streptomyces hachijoensis phospholipase C (1975), J. Biochem., 78, 537-545.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethyl ether | C-1 | Streptomyces hachijoensis |  |
General Stability
| General Stability | Organism |
|---|---|
| sodium dodecyl sulfate | Streptomyces hachijoensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | C-1 | Streptomyces hachijoensis | |
| Ba2+ | C-1 | Streptomyces hachijoensis | |
| Ca2+ | C-1 | Streptomyces hachijoensis | |
| Cu2+ | C-1 | Streptomyces hachijoensis | |
| EDTA | C-1 | Streptomyces hachijoensis | |
| Fe2+ | C-1 | Streptomyces hachijoensis | |
| Fe3+ | C-1 | Streptomyces hachijoensis | |
| SDS | C-1 | Streptomyces hachijoensis | |
| sodium cholate | C-1, at concentrations above 0.02% | Streptomyces hachijoensis | |
| sodium deoxycholate | C-1, at concentrations above 0.02% | Streptomyces hachijoensis | |
| sodium lauryl sulfate | C-1, at concentrations above 0.02% | Streptomyces hachijoensis | |
| Triton X-100 | C-1, greater 0.02% | Streptomyces hachijoensis | |
| Zn2+ | C-1 | Streptomyces hachijoensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
phosphatidylcholine | C-1 | Streptomyces hachijoensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces hachijoensis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Streptomyces hachijoensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
gel filtration, C-1 and C-2 | Streptomyces hachijoensis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| ethyl alcohol | - |
Streptomyces hachijoensis |
| ethyl ether | C-1 activating at a concentration of 20% v/v | Streptomyces hachijoensis |
| ethyl ether-ethanol | - |
Streptomyces hachijoensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hachijoensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| C-1and C-2: dialysis, isoelectric focusing and column chromatography, C-1: 1510foldfold, C-2: 1330fold, both to homogeneity | Streptomyces hachijoensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 74.3 | - |
C-2, substrate: lecithin | Streptomyces hachijoensis |
| 84.6 | - |
C-1, substrate: lecithin | Streptomyces hachijoensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, lyophilized crude preparation, stable for at least 6 months | Streptomyces hachijoensis |
| frozen, neutral pH, almost full activity after 2 years | Streptomyces hachijoensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cardiolipin + H2O | - |
Streptomyces hachijoensis | phosphatidylglycerophosphate + 1,2-diacylglycerol | - |
? | |
| lysophosphatidylcholine + H2O | - |
Streptomyces hachijoensis | monoacylglycerol + phosphorylcholine | - |
? | |
| phosphatidic acid + H2O | - |
Streptomyces hachijoensis | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Streptomyces hachijoensis | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
| phosphatidylethanolamine + H2O | - |
Streptomyces hachijoensis | phosphorylethanolamine + diacylglycerol | - |
? | |
| phosphatidylinositol + H2O | - |
Streptomyces hachijoensis | inositol monophosphate + diacylglycerol | - |
? | |
| phosphatidylserine + H2O | - |
Streptomyces hachijoensis | phosphorylserine + diacylglycerol | - |
? | |
| sphingomyelin + H2O | - |
Streptomyces hachijoensis | ceramide + phosphorylcholine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
C-1 | Streptomyces hachijoensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
pH values between 5.0-9.0, stable for 2 hours, pH values between 6.0-7.0, 95% of activity remains after 24 hours | Streptomyces hachijoensis |
| 37 | - |
for 90 min, 75% of activity remains | Streptomyces hachijoensis |
| 50 | - |
C-1, for 30 min, stable | Streptomyces hachijoensis |
| 70 | - |
complete loss of activity | Streptomyces hachijoensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
C-1, substrate: lecithin | Streptomyces hachijoensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
C-1, stable | Streptomyces hachijoensis |
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