Literature summary for 3.1.4.3 extracted from

Martin, S.F.; Follows, B.C.; Hergenrother, P.J.; Trotter, B.K.
The choline binding site of phospholipase C (Bacillus cereus): insights into substrate specificity (2000), Biochemistry, 39, 3410-3415.

Search for more literature for 3.1.4.3

Activating Compound

Activating Compound	Comment	Organism	Structure
Zn2+	required, three ions per active site	Bacillus cereus

Protein Variants

Protein Variants	Comment	Organism
E4D	significant decrease in catalytic efficiency	Bacillus cereus
E4L	significant decrease in catalytic efficiency	Bacillus cereus
E4Q	significant decrease in catalytic efficiency	Bacillus cereus
F66A	dramatic decrease in catalytic efficiency	Bacillus cereus
F66R	dramatic decrease in catalytic efficiency	Bacillus cereus
F66W	catalytic activity comparable to wild type	Bacillus cereus
Y56A	little decrease in catalytic efficiency	Bacillus cereus
Y56R	little decrease in catalytic efficiency	Bacillus cereus
Y56W	little decrease in catalytic efficiency	Bacillus cereus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.8	-	1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine	pH 7.3	Bacillus cereus
2.4	-	1,2-dihexanoyl-sn-glycero-3-phosphocholine	pH 7.3	Bacillus cereus
4.5	-	1,2-dihexanoyl-sn-glycero-3-phospho-L-serine	pH 7.3	Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28500	-	x * 28500, deduced from gene sequence	Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	P09598	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-dihexanoyl-sn-glycero-3-phospho-L-serine + H2O	-	Bacillus cereus	1,2-dihexanoyl-sn-glycerol + phosphorylserine	-	?
1,2-dihexanoyl-sn-glycero-3-phosphocholine + H2O	-	Bacillus cereus	1,2-dihexanoyl-sn-glycerol + phosphorylcholine	-	?
1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O	-	Bacillus cereus	1,2-dihexanoyl-sn-glycerol + phosphoethanolamine	-	?

Subunits

Subunits	Comment	Organism
?	x * 28500, deduced from gene sequence	Bacillus cereus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)