Information on EC 3.4.23.34 - cathepsin E

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EC Tree 3 Hydrolases 3.4 Acting on peptide bonds (peptidases) 3.4.23 Aspartic endopeptidases 3.4.23.34 cathepsin E

Found in stomach, spleen, erythrocyte membrane; not lysosomal. Pro-cathepsin E is an 86 kDa disulfide-linked dimer; activation or reduction produces monomer. In peptidase family A1 (pepsin A family)

The enzyme appears in viruses and cellular organisms

3.4.23.34
cathepsins
proteinases
pepstatin
pepsinogen
procathepsin
molecular biology
gastricsin
medicine
foveolar
non-lysosomal
3.4.23.5

Reaction Schemes

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similar to cathepsin D, but slightly broader specificity

Synonyms
cathepsin e, cathepsin d-like acid proteinase, cathepsin d-like protease, slow-moving proteinase, slow moving proteinase, non-pepsin proteinase, cathepsin e-like acid proteinase, cathepsin d-like aspartic proteinase, gastric mucosa non-pepsin acid proteinase, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

CatE

3 entries

Cathepsin D-like acid proteinase

cathepsin D-like aspartic proteinase

Meloidogyne incognita

Q1A2Z2

697815

cathepsin D-like protease

2 entries

Cathepsin D-type proteinase

cathepsin E

5 entries

Cathepsin E-like acid proteinase

Homo sapiens

678531

CTSE

2 entries

EMAP

erythrocyte membrane acid proteinase

2 entries

Erythrocyte membrane aspartic proteinase

gastric mucosa non-pepsin acid proteinase

2 entries

Non-pepsin proteinase

slow moving proteinase

2 entries

Slow-moving proteinase

SMP

additional information

CatE

CatE

catE deficient mice develop atopic dermatitis-like skin lesions

679774

CatE

Mus musculus

677973, 677990, 754975

cathepsin D-like protease

Homo sapiens

677990

cathepsin D-like protease

cathepsin E

cathepsin E

cathepsin E

cathepsin E

cathepsin E

CTSE

CTSE

erythrocyte membrane acid proteinase

Homo sapiens

677990

erythrocyte membrane acid proteinase

Mus musculus

677990

gastric mucosa non-pepsin acid proteinase

Homo sapiens

677990

gastric mucosa non-pepsin acid proteinase

Mus musculus

677990

slow moving proteinase

Homo sapiens

677990

slow moving proteinase

Mus musculus

677990

additional information

Cavia porcellus

current names and systematic

30798

additional information

Homo sapiens

current names and systematic

30798

additional information

Oryctolagus cuniculus

current names and systematic

30798

additional information

Rattus norvegicus

current names and systematic

30798

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

similar to cathepsin D, but slightly broader specificity

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

hydrolysis of peptide bond

2 entries

hydrolysis of peptide bond

Homo sapiens

677830

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CAS REGISTRY NUMBER

COMMENTARY

110910-42-4

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 + H2O

Rattus norvegicus

651797

Substrates: fluorogenic synthetic substrate
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O

Rattus norvegicus

667861

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(dinitrophenyl)-D-Arg-NH2

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe + Phe-Arg-Leu-Lys(dinitrophenyl)-D-Arg-NH2

2 entries

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(dinitrophenyl)-D-Arg-NH2 + H2O

Homo sapiens

709450

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe + Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2

Homo sapiens

670765

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O

Rattus norvegicus

669151

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O

3 entries

(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Ser-Ala-Phe-Leu-Ala-Phe-Lys(Dnp)-D-Arg-NH2 + H2O

Rattus norvegicus

667861

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-L-Ala-Gly-L-Phe-L-Ser-L-Leu-L-Pro-L-Ala-L-Lys(Dnp)-D-Arg-amide + H2O

(7-methoxycoumarin-4-yl)acetyl-L-Ala-Gly-L-Phe-L-Ser-L-Leu + L-Pro-L-Ala-L-Lys(Dnp)-D-Arg-amide

Homo sapiens

717271

Substrates: due to the close proximity of a Mca-donor and a Dnp-acceptor, near complete intramolecular quenching effect is achieved in the substrate's intact state. After the proteolytic cleavage of the hydrophobic motif, both Mca and Dnp are further apart, resulting in bright fluorescence
Products: substrate shows a 265fold difference in the net fluorescence signals between cathepsins E and D. This cathepsin E selectivity is established by having Leu-Pro residues at the scissile peptide bond

Acetyl-substance P + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Acetyl-substance P(2-11) + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Acetyl-substance P(3-11) + H2O

Cavia porcellus

30798

Substrates: -
Products: -

acidic fibroblast growth factor + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Acidic fibroblast growth factor fragment 102-111 + H2O

His-Ala-Glu-Lys-His-Trp-Phe + Val-Gly-Leu

2 entries

antigens presented by MHC class II molecules + H2O

Homo sapiens

651042

Substrates: -
Products: -

Arg-modified substance P + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Basic fibroblast growth factor fragment 106-120 + H2O

2 entries

big ET-1 + H2O

2 entries

Bovine gamma-globulin + H2O

Hydrolyzed bovine gamma-globulin

3 entries

Bovine serum albumin + H2O

Mus musculus

755520

Substrates: -
Products: -

Bovine serum albumin + H2O

Hydrolyzed bovine serum albumin

5 entries

casein + H2O

hydrolyzed casein

6 entries

Cholecystokinin 8 + H2O

Asp-Tyr-Met-Gly-Trp + Met-Asp-Phe-NH2

2 entries

Cytochrome c + H2O

Hydrolyzed cytochrome c

Cavia porcellus

30789

Substrates: pH 5, at 2.5% (dimeric enzyme) or 2.3% (monomeric enzyme) the rate of hemoglobin hydrolysis
Products: -

DED-[5-[(2-aminoethyl)amino]naphthalene-1-sulfonyl]-KPILFFRLGK-[4-(4-dimethylaminophenylazo)benzoic acid] + H2O

Homo sapiens

669836

Substrates: -
Products: -

dynorphin A + H2O

hydrolyzed dynorphin A

3 entries

Egg albumin + H2O

Hydrolyzed egg albumin

2 entries

Eledoisin + H2O

Pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe + Ile-Gly-Leu-Met-NH2

2 entries

glucagon + ATP + H2O

hydrolyzed glucagon + ?

Mus musculus

677973

Substrates: is digested at pH 4.0 but not at pH 7.4
Products: -

Hemoglobin + H2O

Rattus norvegicus

651797

Substrates: denatured
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

10 entries

Human beta-endorphin + H2O

Hydrolyzed human beta-endorphin

2 entries

Human endothelin precursor big ET-1 + H2O

Human endothelin precursor ET-1 + respective C-terminal fragment

3 entries

Human endothelin precursor big ET-2 + H2O

Human endothelin precursor ET-2 + respective C-terminal fragment

Homo sapiens

30790

Substrates: cleavage site: Trp-Val
Products: -

Human endothelin precursor big ET-3 + H2O

Huamn endothelin precursor ET-3 + respective C-terminal fragment

Homo sapiens

30790

Substrates: cleavage site: Trp-Ile
Products: -

Human gamma-globulin + H2O

Hydrolyzed human gamma-globulin

Cavia porcellus

30789

Substrates: pH 5, at 0.5% (dimeric enzyme) or 0.6% (monomeric enzyme) the rate of hemoglobin hydrolysis
Products: -

Human renin substrate + H2O

Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Val-Ile-His

2 entries

Immunoglobulin + H2O

Homo sapiens

30792

Substrates: least active gastric protease for this substrate
Products: -

Kassinin + H2O

Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe + Val-Gly-Leu-Met-NH2

2 entries

Lys-Pro-Ala-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ala-Glu-Phe + (4-nitro)Phe-Arg-Leu

Cavia porcellus

30798

Substrates: -
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

10 entries

Membrane proteins + H2O

Homo sapiens

30783

Substrates: -
Products: -

MOCAc-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(DnP)-D-Arg-NH2 + H2O

Rattus norvegicus

P16228

699574

Substrates: -
Products: -

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O

Rattus norvegicus

700517

Substrates: -
Products: -

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe + Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2

Homo sapiens

679774

Substrates: -
Products: -

MOCAc-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O

Mus musculus

754975

Substrates: -
Products: -

MOCAc-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(DNP)-D-Arg-NH2 + H2O

hydrolyzed MOCAc-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(DNP)-D-Arg-NH2

Homo sapiens

678531

Substrates: -
Products: -

N-succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-4-methyl-7-coumaryl-amide + H2O

Meloidogyne incognita

Q1A2Z2

697815

Substrates: -
Products: -

Neurokinin A + H2O

His-Lys-Thr-Asp-Ser-Phe + Val-Gly-Leu-Met-NH2

2 entries

neurotensin + H2O

hydrolyzed neurotensin

Mus musculus

677973

Substrates: no cleavage at pH 7.4
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

7 entries

Porcine renin substrate + H2O

Acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser

2 entries

Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O

Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu

4 entries

Pro-Thr-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Pro-Thr-Glu-Phe + (4-nitro)Phe-Arg-Leu

Cavia porcellus

30798

Substrates: -
Products: -

Reduced and carboxymethylated bovine pancreatic ribonuclease A + H2O

Hydrolyzed bovine pancreatic RCm ribonuclease A

Homo sapiens

30794

Substrates: -
Products: -

reduced carboxymethylated(RCm-)ribonuclease A + H2O

Homo sapiens

653779

Substrates: -
Products: -

renin + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Substance P + H2O

Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2

4 entries

Substance P(1-9) + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Substance P(2-11) + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Substance P(3-11) + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Substance P(4-11) + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Tachykinins + H2O

4 entries

[His10]-substance P + H2O

Cavia porcellus

30798

Substrates: -
Products: -

[Tyr8]-substance P + H2O

Cavia porcellus

30798

Substrates: -
Products: -

additional information

6 entries

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(dinitrophenyl)-D-Arg-NH2

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe + Phe-Arg-Leu-Lys(dinitrophenyl)-D-Arg-NH2

Homo sapiens

677990

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(dinitrophenyl)-D-Arg-NH2

(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe + Phe-Arg-Leu-Lys(dinitrophenyl)-D-Arg-NH2

Mus musculus

677990

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O

Homo sapiens

667861

Substrates: most sensitive and selective substrate for cathepsin E. This substrate might represent a useful tool for monitoring and accurately quantifying cathepsin E, even in crude enzyme preparations
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O

Rattus norvegicus

669151

Substrates: -
Products: -

(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O

Rattus norvegicus

667861

Acidic fibroblast growth factor fragment 102-111 + H2O

His-Ala-Glu-Lys-His-Trp-Phe + Val-Gly-Leu

Cavia porcellus

30798

Substrates: i.e. His-Ala-Glu-Lys-His-Trp-Phe-Val-Gly-Leu or acidic FGF 102-111
Products: -

Acidic fibroblast growth factor fragment 102-111 + H2O

His-Ala-Glu-Lys-His-Trp-Phe + Val-Gly-Leu

Oryctolagus cuniculus

30798

Substrates: i.e. His-Ala-Glu-Lys-His-Trp-Phe-Val-Gly-Leu or acidic FGF 102-111
Products: -

Basic fibroblast growth factor fragment 106-120 + H2O

Cavia porcellus

30798

Substrates: i.e. Tyr-Arg-Ser-Arg-Lys-Tyr-Ser-Ser-Trp-Tyr-Val-Ala-Leu-Lys-Arg or basic FGF 106-120, major cleavage site: Tyr-Val, minor site: Trp-Tyr
Products: -

Basic fibroblast growth factor fragment 106-120 + H2O

Oryctolagus cuniculus

30798

Substrates: i.e. Tyr-Arg-Ser-Arg-Lys-Tyr-Ser-Ser-Trp-Tyr-Val-Ala-Leu-Lys-Arg or basic FGF 106-120, major cleavage site: Tyr-Val, minor site: Trp-Tyr
Products: -

big ET-1 + H2O

Cavia porcellus

30798

Substrates: -
Products: -

big ET-1 + H2O

Homo sapiens

30790, 30798

Substrates: -
Products: -

Bovine gamma-globulin + H2O

Hydrolyzed bovine gamma-globulin

Cavia porcellus

30798

Substrates: -
Products: -

Bovine gamma-globulin + H2O

Hydrolyzed bovine gamma-globulin

Lithobates catesbeianus

30797

Substrates: pH 2.5, at 2.3% the rate of hemoglobin hydrolysis
Products: -

Bovine gamma-globulin + H2O

Hydrolyzed bovine gamma-globulin

Rattus norvegicus

30798

Substrates: -
Products: -

Bovine serum albumin + H2O

Hydrolyzed bovine serum albumin

Cavia porcellus

30798

Substrates: -
Products: -

Bovine serum albumin + H2O

Hydrolyzed bovine serum albumin

Cavia porcellus

30789

Substrates: pH 5, at 3.6% (dimeric enzyme) or 4.1% (monomeric enzyme) the rate of hemoglobin hydrolysis
Products: -

Bovine serum albumin + H2O

Hydrolyzed bovine serum albumin

Homo sapiens

30798

Substrates: -
Products: -

Bovine serum albumin + H2O

Hydrolyzed bovine serum albumin

Lithobates catesbeianus

30797

Substrates: pH 2.5 at 14% the rate of hemoglobin hydrolysis
Products: -

Bovine serum albumin + H2O

Hydrolyzed bovine serum albumin

Rattus norvegicus

30798

Substrates: -
Products: -

casein + H2O

hydrolyzed casein

Cavia porcellus

30798

Substrates: -
Products: -

casein + H2O

hydrolyzed casein

Cavia porcellus

30789

Substrates: pH 5, at 9.1% (dimeric enzyme) or 8.9% (monomeric enzyme) the rate of hemoglobin hydrolysis
Products: -

casein + H2O

hydrolyzed casein

Homo sapiens

30784, 30798

Substrates: -
Products: -

casein + H2O

hydrolyzed casein

Homo sapiens

30793

Substrates: at pH 5.5
Products: -

casein + H2O

hydrolyzed casein

Lithobates catesbeianus

30797

Substrates: at 2.3% the rate of hemoglobin hydrolysis
Products: -

casein + H2O

hydrolyzed casein

Rattus norvegicus

30798

Substrates: -
Products: -

Cholecystokinin 8 + H2O

Asp-Tyr-Met-Gly-Trp + Met-Asp-Phe-NH2

Cavia porcellus

30798

Substrates: i.e. Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2, cleavage site: Trp-Met
Products: -

Cholecystokinin 8 + H2O

Asp-Tyr-Met-Gly-Trp + Met-Asp-Phe-NH2

Oryctolagus cuniculus

30798

Substrates: i.e. Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2, cleavage site: Trp-Met
Products: -

dynorphin A + H2O

hydrolyzed dynorphin A

Cavia porcellus

30798

Substrates: cleavage site: Phe4-Leu5
Products: -

dynorphin A + H2O

hydrolyzed dynorphin A

Mus musculus

677973

Substrates: no cleavage at pH 7.4
Products: -

dynorphin A + H2O

hydrolyzed dynorphin A

Oryctolagus cuniculus

30798

Substrates: cleavage site: Phe4-Leu5
Products: -

Egg albumin + H2O

Hydrolyzed egg albumin

Cavia porcellus

30789

Substrates: pH 5, at 1% (dimeric enzyme) or 1.1% (monomeric enzyme) the rate of hemoglobin hydrolysis
Products: -

Egg albumin + H2O

Hydrolyzed egg albumin

Cavia porcellus

30798

Substrates: i.e. ovalbumin
Products: -

Eledoisin + H2O

Pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe + Ile-Gly-Leu-Met-NH2

Cavia porcellus

30798

Substrates: i.e. pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe-Ile-Gly-Leu-Met-NH2, cleavage site: Phe-Ile
Products: -

Eledoisin + H2O

Pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe + Ile-Gly-Leu-Met-NH2

Oryctolagus cuniculus

30798

Substrates: i.e. pyro-Glu-Pro-Ser-Lys-Asp-Ala-Phe-Ile-Gly-Leu-Met-NH2, cleavage site: Phe-Ile
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Cavia porcellus

30798

Substrates: -
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Cavia porcellus

30789

Substrates: preferred substrate
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Cavia porcellus

30789

Substrates: at pH 2 the two catalytically active subunits have the same activity vs. hemoglobin, but at pH 5 they have a slightly higher activity than the enzyme dimer
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Homo sapiens

30793, 30795, 30796, 30798

Substrates: -
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Homo sapiens

30778, 30783, 30799

Substrates: acid denatured form
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Homo sapiens

30778, 30799

Substrates: bovine
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Lithobates catesbeianus

30797

Substrates: preferred substrate
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Rattus norvegicus

30779, 30798

Substrates: -
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Rattus norvegicus

30778, 30803

Substrates: acid denatured form
Products: -

Hemoglobin + H2O

Hydrolyzed hemoglobin

Rattus norvegicus

30778, 30803

Substrates: bovine
Products: -

Human beta-endorphin + H2O

Hydrolyzed human beta-endorphin

Cavia porcellus

30798

Substrates: major cleavage site: Leu17-Phe18, minor site: Thr16-Leu17
Products: -

Human beta-endorphin + H2O

Hydrolyzed human beta-endorphin

Oryctolagus cuniculus

30798

Substrates: major cleavage site: Leu17-Phe18, minor site: Thr16-Leu17
Products: -

Human endothelin precursor big ET-1 + H2O

Human endothelin precursor ET-1 + respective C-terminal fragment

Cavia porcellus

30798

Substrates: cleavage site: Trp-Val
Products: -

Human endothelin precursor big ET-1 + H2O

Human endothelin precursor ET-1 + respective C-terminal fragment

Homo sapiens

30790, 30798

Substrates: cleavage site: Trp-Val
Products: -

Human endothelin precursor big ET-1 + H2O

Human endothelin precursor ET-1 + respective C-terminal fragment

Oryctolagus cuniculus

30798

Substrates: cleavage site: Trp-Val
Products: -

Human renin substrate + H2O

Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Val-Ile-His

Cavia porcellus

30798

Substrates: i.e. angiotensinogen fragment 1-13 or Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Val-Ile-His, cleavage site: Leu-Val
Products: -

Human renin substrate + H2O

Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Val-Ile-His

Oryctolagus cuniculus

30798

Substrates: i.e. angiotensinogen fragment 1-13 or Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Val-Ile-His, cleavage site: Leu-Val
Products: -

Kassinin + H2O

Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe + Val-Gly-Leu-Met-NH2

Cavia porcellus

30798

Substrates: i.e. Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val
Products: -

Kassinin + H2O

Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe + Val-Gly-Leu-Met-NH2

Oryctolagus cuniculus

30798

Substrates: i.e. Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Cavia porcellus

30798

Substrates: synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Homo sapiens

30780, 30784, 30793, 30795, 30796, 30801, 30802

Substrates: -
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Homo sapiens

30798

Substrates: synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Homo sapiens

30787

Substrates: pH 7.4
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Homo sapiens

30787

Substrates: no activation by ATP
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Oryctolagus cuniculus

30780

Substrates: -
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Oryctolagus cuniculus

30798

Substrates: synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Rattus norvegicus

30780

Substrates: -
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Rattus norvegicus

30798

Substrates: synthetic chromogenic peptide, less suitable peptide substrate than substance P or other tachykinins
Products: -

Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O

Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu

Rattus norvegicus

30803

Substrates: i.e. RS-6
Products: -

Neurokinin A + H2O

His-Lys-Thr-Asp-Ser-Phe + Val-Gly-Leu-Met-NH2

Cavia porcellus

30798

Substrates: i.e. His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val
Products: -

Neurokinin A + H2O

His-Lys-Thr-Asp-Ser-Phe + Val-Gly-Leu-Met-NH2

Oryctolagus cuniculus

30798

Substrates: i.e. His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2, cleavage site: Phe-Val
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

Cavia porcellus

30798

Substrates: cleavage sites
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

Homo sapiens

30794

Substrates: -
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

Homo sapiens

30798

Substrates: cleavage sites
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

Homo sapiens

30787

Substrates: no activation by ATP
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

Homo sapiens

30787

Substrates: cleavage specificity changes significantly with pH, e.g. cleaves Glu13-Ala14 only at pH 7.4
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

Oryctolagus cuniculus

30798

Substrates: cleavage sites
Products: -

Oxidized insulin B-chain + H2O

Hydrolyzed oxidized insulin B-chain

Rattus norvegicus

30798

Substrates: cleavage sites
Products: -

Porcine renin substrate + H2O

Acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser

Cavia porcellus

30798

Substrates: i.e. angiotensinogen fragment 1-14 acetate salt or acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser, cleavage site: Leu-Leu
Products: -

Porcine renin substrate + H2O

Acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + Leu-Val-Tyr-Ser

Oryctolagus cuniculus

30798

Substrates: i.e. angiotensinogen fragment 1-14 acetate salt or acetyl-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser, cleavage site: Leu-Leu
Products: -

Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O

Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu

Cavia porcellus

30798

Substrates: -
Products: -

Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O

Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu

Homo sapiens

30795, 30796, 30798

Substrates: -
Products: -

Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O

Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu

Homo sapiens

30780, 30784, 30793, 30801, 30802

Substrates: synthetic chromogenic peptide
Products: -

Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O

Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu

Rattus norvegicus

30780

Substrates: synthetic chromogenic peptide
Products: -

Substance P + H2O

Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2

Cavia porcellus

30798

Substrates: i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe
Products: -

Substance P + H2O

Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2

Homo sapiens

30798

Substrates: i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe
Products: -

Substance P + H2O

Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2

Oryctolagus cuniculus

30798

Substrates: i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe
Products: -

Substance P + H2O

Arg-Pro-Lys-Pro-Gln-Gln-Phe + Phe-Gly-Leu-Met-NH2

Rattus norvegicus

30798

Substrates: i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, best peptide substrate, cleavage site: Phe-Phe
Products: -

Tachykinins + H2O

Cavia porcellus

30798

Substrates: -
Products: -

Tachykinins + H2O

Homo sapiens

30798

Substrates: -
Products: -

Tachykinins + H2O

Oryctolagus cuniculus

30798

Substrates: -
Products: -

Tachykinins + H2O

Rattus norvegicus

30798

Substrates: -
Products: -

additional information

Homo sapiens

30790

Substrates: no hydrolysis of synthetic peptides corresponding to residues His16-His27 and His16-Ser38 in the big ET-1 sequence
Products: -

additional information

Homo sapiens

30792

Substrates: milk-clotting activity is twice as high as that of pepsinogen and gastricin
Products: -

additional information

Homo sapiens

651042

Substrates: degradation of protein antigens, crucial step in the initiation of a T-cell mediated immune response
Products: -

additional information

Homo sapiens

669718

Substrates: cathepsin E has an important role in the class II MHC Ag processing pathway within dendritic cells
Products: -

additional information

Mus musculus

669718

Substrates: cathepsin E has an important role in the class II MHC Ag processing pathway within dendritic cells
Products: -

additional information

Mus musculus

669583

Substrates: cathepsin E is located in mast-cell secretory granules in complex with heparin proteoglycans, and has a role in processing of procarboxypeptidase A into active protease
Products: -

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

Bovine serum albumin + H2O

Mus musculus

755520

Substrates: -
Products: -

MOCAc-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O

Mus musculus

754975

Substrates: -
Products: -

additional information

4 entries

additional information

Homo sapiens

651042

Substrates: degradation of protein antigens, crucial step in the initiation of a T-cell mediated immune response
Products: -

additional information

Homo sapiens

669718

Substrates: cathepsin E has an important role in the class II MHC Ag processing pathway within dendritic cells
Products: -

additional information

Mus musculus

669718

Substrates: cathepsin E has an important role in the class II MHC Ag processing pathway within dendritic cells
Products: -

additional information

Mus musculus

669583

Substrates: cathepsin E is located in mast-cell secretory granules in complex with heparin proteoglycans, and has a role in processing of procarboxypeptidase A into active protease
Products: -

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

ATP

13 entries

CTP

Homo sapiens

activation, maintains enzyme in its active conformation at pH 5 and above

ATP

ATP

activation

30784, 30793, 30801

ATP

Homo sapiens

maintains enzyme in its active conformation at pH 5 and above

30784

ATP

Homo sapiens

hemoglobin as substrate

ATP

6.25 mM

ATP

at weakly acidic and neutral pH

30798

ATP

Homo sapiens

at weakly acidic and neutral pH

30798

ATP

Rattus norvegicus

at weakly acidic and neutral pH

30798

ATP

Oryctolagus cuniculus

at weakly acidic and neutral pH

30798

ATP

Homo sapiens

activates native, not recombinant enzyme

30801

ATP

Homo sapiens

no activation of casein hydrolysis at pH 5.5

30793

ATP

Homo sapiens

no activation at low pH-values, e.g. pH 4.5

30784

ATP

Homo sapiens

no activation at pH 7.4

30787

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

Homo sapiens

no activation by Mg2+ or vanadate

30784

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

(D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr

2 entries

1,2-epoxy-3-(p-nitrophenoxy)propane

4 entries

alpha2-Macroglobulin

Homo sapiens

at pH 5.5, RNAse as substrate, at a molar ratio of enzyme/inhibitor of 0.5:1 to 2:1, above a ratio of 2:1 the excess enzyme is not inhibited, structural changes in alpha2-macroglobulin upon complex formation, no inhibition with oxidized insulin B-chain as substrate

30794

Ascaris pepsin inhibitor

3 entries

CCACCAACACAACTAAACTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 4.5% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCACCACCACAACAAAACTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 31.0% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCACCACCACAACGAAACTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 14.0% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCACCACCACAATAAAACTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 33.5% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCATCACTACAACAAAACTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 11.0% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCCATAGGGATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 22.3% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCCATAGTGCTCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 6.2% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCCCCACCACAACCCTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 37.6% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

CCCCCACCACAACCCTCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 33.3% inhibition (2 nM inhibitor /2 nM cathepsin E)

DGCCIIIIGGRPPTHLFLD

DGCCIIINGGRPPTVFFRVKDYKHHDDK

Rattus norvegicus

P16228

699574

Diazoacetyl-DL-norleucine methyl ester

GCGGLLPFGGQPPNPLF

GGRPIIIIGG

GGSCSSCLGGRPPTIFFRLKDYKDDDDK

grassystatin A

potent cathepsin E inhibitor, shows selectivity for cathepsin E over cathepsin D

709450

grassystatin B

Homo sapiens

potent cathepsin E inhibitor, shows selectivity for cathepsin E over cathepsin D

709450

grassystatin C

Homo sapiens

potent cathepsin E inhibitor, shows selectivity for cathepsin E over cathepsin D

IIIISCIGDDGHQKKKK

N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2

4 entries

N-tert-Butoxycarbonyl-His-Pro-Phe-His-Leu-PSI(CHOH-CH2)-Val-Ile-His

NDDKIIIICCII

NDDKIIIIPTIFGG

NYKDSCIG

PepA-penetratin

pepstatin

pepstatin A

Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu

4 entries

Pro-Thr-Glu-Phe-PSI(CH2-NH)-Phe-Arg-Glu

2 entries

Protein inhibitor from Ascaris lumbricoides

SCGGIIIISCIA

SCIGIIDSGGRPPTIFFRAEGLQR

SGLLFRLKGG

TCCATAAGGATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 23.2% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCATAGGAATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 26.8% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCATAGGGATTCACTCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 17.7% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCATAGGGCTCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 24.0% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCATAGGGTCACTCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 18.4% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCATAGGTATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 17.8% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCATCGGGATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 15.4% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCCCGGAGCTCACTCATCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 27.0% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCCCGGAGCTCACTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 11.5% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TCCCCGGTGCTCACTTATCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 19.3% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TGCATCGGGATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 40.8% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

Tripeptide analogs

4 entries

TTCATATGGATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 23.0% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

TTCATCGGGATCACTCCCTCCAC

Rattus norvegicus

inhibitory part, additionally the molecule has the conserved sequence GATCTCACTCCTTCGCAGTATTCGCGAGCC at its 5'-side, 29.7% inhibition (2 nM inhibitor /2 nM cathepsin E)

700517

YCGGLLPLGGRPPTIFFRLKDYKGDDDK

Rattus norvegicus

P16228

699574

additional information

5 entries

(D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr

Homo sapiens

i.e. H-77, with reduced isostere as replacement of the-CO-NH- of the peptide bond

30780, 30790, 30796

(D)-His-Pro-Phe-His-Leu-PSI(CH2-NH)-Leu-Val-Tyr