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Literature summary for 3.4.23.34 extracted from

  • Athauda, S.B.P.; Takahashi, T.; Inoue, H.; Ichinose, M.; Takahashi, K.
    Proteolytic activity and cleavage specificity of cathepsin E at the physiological pH as examined towards the B chain of oxidized insulin (1991), FEBS Lett., 292, 53-56.
    View publication on PubMed
Search for more literature for 3.4.23.34

Inhibitors

Inhibitors Comment Organism Structure
additional information 1,10-phenanthroline, diprotin A, antipain, amastatin, L-trans-epoxysuccinyl-leucylamido-(4-guanidino)-butane (i.e. E-64, at pH 7.4); inhibition by PMSF, diisopropyl fluorophosphate, leupeptin Homo sapiens
pepstatin as substrate, incubation at pH 7.4 restores; at pH 3 and 5.5, not at pH 7.4 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O pH 7.4 Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O no activation by ATP Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Oxidized insulin B-chain + H2O no activation by ATP Homo sapiens Hydrolyzed oxidized insulin B-chain
-
 ?
Oxidized insulin B-chain + H2O cleavage specificity changes significantly with pH, e.g. cleaves Glu13-Ala14 only at pH 7.4 Homo sapiens Hydrolyzed oxidized insulin B-chain
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 approximate value Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP no activation at pH 7.4 Homo sapiens