EC Number |
Protein Variants |
Reference |
---|
3.4.23.34 | D283A |
site-directed mutagenesis |
651797 |
3.4.23.34 | D283E |
site-directed mutagenesis |
651797 |
3.4.23.34 | D98A |
site-directed mutagenesis |
651797 |
3.4.23.34 | D98A/D283A |
double mutant, site-directed mutagenesis |
651797 |
3.4.23.34 | D98A/D283A |
expression in HEK293T cells |
679028 |
3.4.23.34 | D98A/D283A |
mutant enzyme has no catalytic activity on either protein or synthetic substrates. In contrast with wild-type cathepsin E, the mutant enzyme is neither processed nor matured even after a 24-h chase period, but stably remains as a 46000 Da precursor |
668563 |
3.4.23.34 | D98E |
site-directed mutagenesis |
651797 |
3.4.23.34 | D98E/D283E |
double mutant, site-directed mutagenesis |
651797 |
3.4.23.34 | more |
construction of two fusion proteins using chimeric DNAs encoding the cathepsin E propeptide fused to the mature cathepsin D tagged with HA at the COOH terminus and encoding the cathepsin D propeptide fused to the mature cathepsin E |
669151 |
3.4.23.34 | N324D |
N-glycosylation-deficient mutant is neither processed into a mature form nor transported to the endosomal compartement, but is stable retained in the endoplasmic reticulum without degradation |
668563 |