Cloned (Comment) | Organism |
---|---|
construction of two fusion proteins using chimeric DNAs encoding the cathepsin E propeptide fused to the mature cathepsin D tagged with HA at the COOH terminus and encoding the cathepsin D propeptide fused to the mature cathepsin E | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of two fusion proteins using chimeric DNAs encoding the cathepsin E propeptide fused to the mature cathepsin D tagged with HA at the COOH terminus and encoding the cathepsin D propeptide fused to the mature cathepsin E | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ascaris pepsin inhibitor | - |
Rattus norvegicus | |
pepstatin A | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00153 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 | 40°C, pH 4.0, wild-type cathepsin E | Rattus norvegicus | |
0.0028 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 | 40°C, pH 4.0, wild-type cathepsin E | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | propeptide of cathepsin E is essential for the correct folding, maturation, and targeting of this protein to its final destination | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 | 40°C, pH 4.0, wild-type cathepsin E | Rattus norvegicus | |
13.58 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 | 40°C, pH 4.0, wild-type cathepsin E | Rattus norvegicus | |
32.2 | - |
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 | 40°C, pH 4.0, wild-type cathepsin E | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000031 | - |
pepstatin A | 40°C, pH 4.0, wild-type cathepsin E | Rattus norvegicus | |
0.0000135 | - |
Ascaris pepsin inhibitor | 40°C, pH 4.0, wild-type cathepsin E | Rattus norvegicus |