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Literature summary for 3.4.23.34 extracted from

  • Liu, J.; Tsukuba, T.; Okamoto, K.; Ohishi, M.; Yamamoto, K.
    Mutational analysis of residues in two consensus motifs in the active sites of cathepsin E (2002), J. Biochem., 132, 493-499.
    View publication on PubMed
Search for more literature for 3.4.23.34

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in human embryonic kidney 293T cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D283A site-directed mutagenesis Rattus norvegicus
D283E site-directed mutagenesis Rattus norvegicus
D98A site-directed mutagenesis Rattus norvegicus
D98A/D283A double mutant, site-directed mutagenesis Rattus norvegicus
D98E site-directed mutagenesis Rattus norvegicus
D98E/D283E double mutant, site-directed mutagenesis Rattus norvegicus
T284S site-directed mutagenesis Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ascaris pepsin inhibitor
-
 Rattus norvegicus
pepstatin A
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.27
-
 (7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 pH 3.5, 40°C, wild-type Rattus norvegicus
1.31
-
 (7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 pH 3.5, 40°C, mutant D98E Rattus norvegicus
1.91
-
 (7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 pH 3.5, 40°C, mutant T284S Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Rattus norvegicus 5622
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and T284S and D98E mutant proteins Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 + H2O fluorogenic synthetic substrate Rattus norvegicus ?
-
 ?
Hemoglobin + H2O denatured Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.55
-
 (7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 pH 3.5, 40°C, mutant D98E Rattus norvegicus
6.4
-
 (7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 pH 3.5, 40°C, mutant T284S Rattus norvegicus
11.9
-
 (7-methoxycoumarin-4-yl)-acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(2,4-dinitrophenyl)-D-Arg-NH2 pH 3.5, 40°C, wild-type Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000582
-
 pepstatin A pH 3.5, 40°C, wild-type Rattus norvegicus
0.00000606
-
 pepstatin A pH 3.5, 40°C, mutant D98E Rattus norvegicus
0.00000648
-
 pepstatin A pH 3.5, 40°C, mutant T284S Rattus norvegicus
0.00001834
-
 Ascaris pepsin inhibitor pH 3.5, 40°C, wild-type Rattus norvegicus
0.00001913
-
 Ascaris pepsin ihibitor pH 3.5, 40°C, mutant D98E Rattus norvegicus
0.00001953
-
 Ascaris pepsin inhibitor pH 3.5, 40°C, mutant T284S Rattus norvegicus