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Literature summary for 3.4.23.34 extracted from

  • Thomas, D.J.; Richards, A.D.; Jupp, R.A.; Ueno, E.; Yamamoto, K.; Samloff, I.M.; Dunn, B.M.; Kay, J.
    Stabilisation of cathepsin E by ATP (1989), FEBS Lett., 243, 145-148.
    View publication on PubMed
Search for more literature for 3.4.23.34

Activating Compound

Activating Compound Comment Organism Structure
GTP activation, maintains enzyme in its active conformation at pH 5 and above Homo sapiens
additional information or 2-mercaptoethanol Homo sapiens

General Stability

General Stability Organism
ATP, CTP or GTP stabilizes in the range of pH 5-7.4, not below pH 5, adenosine, sodium triphosphate, ADP, 2,3-diphosphoglycerate do not stabilize Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information 2-mercaptoethanol, Mg2+ or vanadate Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by Mg2+ or vanadate Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gastric mucosa
-
 Homo sapiens
-
stomach
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Homo sapiens hydrolyzed casein
-
 ?
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O synthetic chromogenic peptide Homo sapiens Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information pH-dependence of kinetic constants Homo sapiens
5
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu value below, with ATP, pH 7 Homo sapiens
11
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 6.6 and without ATP, pH 5 Homo sapiens
16
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 6.2 Homo sapiens
26
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu without ATP, pH 5.4 Homo sapiens
31
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 5 Homo sapiens
36
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu without ATP, pH 4.5 Homo sapiens
39
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 4.5 and pH 5.4 Homo sapiens
40
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 5.8 Homo sapiens
47
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu without ATP, pH 5 Homo sapiens
54
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu without ATP, pH 5 Homo sapiens
56
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 5.8 Homo sapiens
72
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 5.4 Homo sapiens
76
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu without ATP, pH 4.5 Homo sapiens
77
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu with ATP, pH 4.5 and pH 5 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 7 active in the presence of ATP Homo sapiens
5.8
-
 and above, no activity in the absence of ATP Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP activation Homo sapiens
ATP maintains enzyme in its active conformation at pH 5 and above Homo sapiens
ATP no activation at low pH-values, e.g. pH 4.5 Homo sapiens
CTP activation, maintains enzyme in its active conformation at pH 5 and above Homo sapiens