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Literature summary for 3.4.23.34 extracted from

  • Tsukuba, T.; Masayuki, Y.; Hori, H.; Azuma, T.; Yamamoto, K.
    Comparison of biochemical properties of natural and recombinant cathepsin E (1995), Adv. Exp. Med. Biol., 362, 331-334.
    View publication on PubMed
Search for more literature for 3.4.23.34

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Chinese hamster ovary cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-tert-Butoxycarbonyl-His-Pro-Phe-His-4-amino-3-hydroxy-6-methylheptanoic acid-Leu-Phe-NH2
-
 Homo sapiens
pepstatin also inhibits conversion of procathepsin E to cathepsin E Homo sapiens
Pro-Thr-Glu-Phe-PSI(CH2-NH)-Nle-Arg-Leu
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information recombinant enzyme is catalytically indistinguishable from natural cathepsin Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm recombinant enzyme Homo sapiens 5737
-
endoplasmic reticulum recombinant enzyme Homo sapiens 5783
-
soluble
-
 Homo sapiens
-
-
vacuole recombinant enzyme Homo sapiens 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 human, SDS-PAGE, non-reducing conditions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzymes: N-glycosylated with high-mannose type oligosaccharide chain Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O synthetic chromogenic peptide Homo sapiens Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 20 min, inactivation of native enzyme at pH 5.5, only 10% loss of recombinant enzyme activity Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP activation Homo sapiens
ATP activates native, not recombinant enzyme Homo sapiens