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Literature summary for 3.4.23.34 extracted from

  • Fowler, S.D.; Kay, J.; Dunn, B.M.; Tatnell, P.J.
    Monomeric human cathepsin E (1995), FEBS Lett., 366, 72-74.
    View publication on PubMed
Search for more literature for 3.4.23.34

General Stability

General Stability Organism
Freezing inactivates, 50% glycerol stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
pepstatin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu C4A mutant cathepsin E, pH 3.1 Homo sapiens
0.045
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu C4A mutant cathepsin E, pH 3.1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
monomeric form Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Homo sapiens Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu + H2O synthetic chromogenic peptide Homo sapiens Pro-Pro-Thr-Ile-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the temperature stability of the mutant monomeric form in alkaline solution is markedly reduced Homo sapiens
25
-
 and below, stable, t1/2: more than 3 h at pH 9.5 Homo sapiens
37
-
 t1/2: about 16 h at pH 8.5, t1/2: 40 min at pH 9.5, t1/2: 3 min at pH 10.5 Homo sapiens
45
-
 t1/2: 40 min at pH 8.5 Homo sapiens
55
-
 t1/2: 35 min (native enzyme) or 1.7 min (recombinant enzyme) at pH 7.5, t1/2: less than 5 min at pH 8.5 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
70
-
 Pro-Pro-Thr-Ile-Phe-(4-nitro)Phe-Arg-Leu Cys4-Ala mutant cathepsin E, pH 3.1 Homo sapiens
82
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu Cys4-Ala mutant cathepsin E, pH 3.1 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 the pH-stability of the mutant monomeric form in alkaline solution is markedly reduced Homo sapiens