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Literature summary for 3.4.23.34 extracted from

  • Iida, H.; Matsuba, T.; Yamada, M.; Azuma, T.; Suzuki, H.; Yamamoto, K.; Hori, H.
    Purification and characterization of recombinant human cathepsin E (1995), Adv. Exp. Med. Biol., 362, 325-330.
    View publication on PubMed
Search for more literature for 3.4.23.34

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris cells Homo sapiens
human Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, recombinant enzyme, SDS-PAGE, reducing conditions Homo sapiens
84000
-
 human, recombinant enzyme, SDS-PAGE, non-reducing conditions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzymes: N-glycosylated with high-mannose type oligosaccharide chain Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant from Pichia pastoris Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O acid denatured form Homo sapiens Hydrolyzed hemoglobin
-
 ?
Hemoglobin + H2O bovine Homo sapiens Hydrolyzed hemoglobin
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, recombinant enzyme, SDS-PAGE, reducing conditions Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5 3.6 hemoglobin as substrate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
2 4 90% of maximal activity at pH 2 and half-maximal activity at pH 4, hemoglobin as substrate Homo sapiens