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Literature summary for 3.4.23.34 extracted from

  • Yasuda, Y.; Kohmura, K.; Kadowaki, T.; Tsukuba, T.; Yamamoto, K.
    A new selective substrate for cathepsin E based on the cleavage site sequence of alpha2-macroglobulin (2005), Biol. Chem., 386, 299-305.
    View publication on PubMed
Search for more literature for 3.4.23.34

Inhibitors

Inhibitors Comment Organism Structure
Ascaris pepsin inhibitor
-
 Homo sapiens
Ascaris pepsin inhibitor
-
 Rattus norvegicus
pepstatin A
-
 Homo sapiens
pepstatin A
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00179
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin E Rattus norvegicus
0.0019
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, erythrocyte cathepsin E Homo sapiens
0.00228
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, erythrocyte cathepsin E Rattus norvegicus
0.00243
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Ser-Ala-Phe-Leu-Ala-Phe-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin E Rattus norvegicus
0.0032
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin E Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
erythrocyte
-
 Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-
stomach
-
 Rattus norvegicus
-
thymus
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 + H2O
-
 Rattus norvegicus ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O most sensitive and selective substrate for cathepsin E. This substrate might represent a useful tool for monitoring and accurately quantifying cathepsin E, even in crude enzyme preparations Homo sapiens ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 + H2O most sensitive and selective substrate for cathepsin E. This substrate might represent a useful tool for monitoring and accurately quantifying cathepsin E, even in crude enzyme preparations Rattus norvegicus ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Ser-Ala-Phe-Leu-Ala-Phe-Lys(Dnp)-D-Arg-NH2 + H2O
-
 Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.68
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Ser-Ala-Phe-Leu-Ala-Phe-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin E Rattus norvegicus
18.8
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, erythrocyte cathepsin E Rattus norvegicus
19.4
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, erythrocyte cathepsin E Homo sapiens
20.1
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin E Rattus norvegicus
39.1
-
 (7-methoxycoumarin-4-yl)acetyl-Gly-Lys-Pro-Ile-Ile-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 40°C, pH 4.0, gastric cathepsin E Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
2 5 pH 2.0 and 5.0: approximately 80% of maximal activity Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000056
-
 pepstatin A 40°C, pH 4.0, erythrocyte cathepsin E, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Rattus norvegicus
0.0000078
-
 pepstatin A 40°C, pH 4.0, gastric cathepsin E, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Rattus norvegicus
0.0000084
-
 pepstatin A 40°C, pH 4.0, erythrocyte cathepsin E, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Homo sapiens
0.0000112
-
 Ascaris pepsin inhibitor 40°C, pH 4.0, erythrocyte cathepsin E, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Rattus norvegicus
0.0000145
-
 Ascaris pepsin inhibitor 40°C, pH 4.0, erythrocyte cathepsin E, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Homo sapiens
0.0000165
-
 Ascaris pepsin inhibitor 40°C, pH 4.0, gastric cathepsin E, hydrolysis of (7-methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-NH2 Rattus norvegicus