Information on EC 3.1.26.5 - ribonuclease P

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.1.26.5
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RECOMMENDED NAME
GeneOntology No.
ribonuclease P
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tRNA processing
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CAS REGISTRY NUMBER
COMMENTARY hide
71427-00-4
not distinguished from EC 3.1.26.7
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain 1A40
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Manually annotated by BRENDA team
enzyme is a catalytically active RNA, at least 2 isoforms with different substrate specificities
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Manually annotated by BRENDA team
fragment
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Manually annotated by BRENDA team
calf
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
ribonuclease P protein component; subspecies Mycoplasma mycoides mycoides SC
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Aquifex aeolicus
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Manually annotated by BRENDA team
no activity in Nanoarchaeum equitans
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
component RNPA
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
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the enzyme is involved in maturation of the 5'-end of tRNA
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10Sa RNA + H2O
?
show the reaction diagram
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?
4.55 rRNA precursor + H2O
mature 4.55 rRNA + 5'-oligonucleotide
show the reaction diagram
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?
4.5S RNA precursor + H2O
mature 4.5S RNA + 5'-oligonucleotide
show the reaction diagram
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RNA processing
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?
4.5S RNA, precursor to + H2O
?
show the reaction diagram
C4 antisense RNA + H2O
?
show the reaction diagram
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from bacteriophages P1 and P7
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?
chloroplastic pre-tRNAPhe + H2O
chloroplastic tRNAPhe + 5' leader of tRNA
show the reaction diagram
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?
deproteinized pre-rRNA + H2O
mature deproteinized rRNA + 5'-oligonucleotide
show the reaction diagram
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large number of discrete cleavage sites
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?
hepatitis C virus RNA + H2O
?
show the reaction diagram
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the catalytic RNase P RNA cleaves near the AUG start codon
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?
influenza virus mRNA + H2O
?
show the reaction diagram
mitochondrial pre-tRNACys + H2O
mitochondrial tRNACys + 5' leader of tRNA
show the reaction diagram
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?
pbuE adenine riboswitch + H2O
?
show the reaction diagram
phage antisense RNA (C4) + H2O
?
show the reaction diagram
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?
phage f2RNA + H2O
?
show the reaction diagram
phi80-induced RNA + H2O
?
show the reaction diagram
polycistronic his operon mRNA precursor + H2O
?
show the reaction diagram
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?
polycistronic mRNA precursor + H2O
mature mRNAs + ?
show the reaction diagram
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RNA processing
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?
pre-rRNA + H2O
?
show the reaction diagram
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RNase MRP is involved with the maturation of pre-rRNA but cleaves RNA primers in mitochondria and localizes to cytoplasmic P-bodies where it takes part in cell cycle-regulated turnover of selected mRNAs
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?
pre-rRNA + H2O
mature rRNA
show the reaction diagram
pre-rRNA + H2O
mature rRNA + 5'-oligonucleotide
show the reaction diagram
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RNase MRP
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?
pre-tRNA + H2O
?
show the reaction diagram
pre-tRNA + H2O
mat-tRNA + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNA + H2O
mature tRNA + 5'-terminal oligonucleotide
show the reaction diagram
pre-tRNA + H2O
tRNA
show the reaction diagram
pre-tRNA + H2O
tRNA + 5' leader of tRNA
show the reaction diagram
pre-tRNA + H2O
tRNA + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA + H2O
tRNA + RNA sequence
show the reaction diagram
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a pre-tRNA is trapped on the CCA site and 5'-leader site first to form the Michaelis-complex. On this step, the shape of a substrate RNA is not recognized by the enzyme. After that, the T-arm site and the bottom half site cooperatively examine the shape of the substrate to achieve the transition state conformation. After the cleavage of the 5'-leader sequence, the enzyme-product complex turns to the non-transition state conformation, and the cleaved product is released from the enzyme
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?
pre-tRNA mimic + H2O
?
show the reaction diagram
pre-tRNA mimic a + H2O
?
show the reaction diagram
pre-tRNA mimic b + H2O
?
show the reaction diagram
pre-tRNA mimic c + H2O
?
show the reaction diagram
pre-tRNA mimic d + H2O
?
show the reaction diagram
pre-tRNA precursor + H2O
mat-tRNA + RNA
show the reaction diagram
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?
pre-tRNA precursor + H2O
tRNA + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA(Tyr) + H2O
mature tRNA(Tyr) + 5'-terminal oligonucleotide
show the reaction diagram
pre-tRNA-Gly + H2O
tRNA-Gly + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA-Met + H2O
tRNA-Met + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNA-Tyr + H2O
tRNA-Tyr + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA-Val + H2O
tRNA-Val + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNA3Pro + H2O
mature tRNA3Pro + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNAAla + H2O
mature tRNAAla + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of fly substrate, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNAAsp + H2O
?
show the reaction diagram
pre-tRNAAsp + H2O
tRNAAsp + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNAAsp + H2O
tRNAsp + 5'-oligoribonucleotide
show the reaction diagram
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pre-tRNA binding affinities for RNase P are enhanced by sequence-specific contacts between the fourth pre-tRNA nucleotide on the 5' side of the cleavage site (N(-4)) and the RNase P protein (P protein) subunit. RNase P has a higher affinity for pre-tRNA with adenosine at N(-4), and this binding preference is amplified at physiological divalent ion concentrations. Binds A(-4) pre-tRNA 20fold more tightly than the G(-4) substrate, and binds the C(-4) and U(-4) substrates with intermediate affinity. F20 and Y34 contribute to selectivity at N(-4). The hydroxyl group of Y34 enhances selectivity, likely by forming a hydrogen bond with the N(-4) nucleotide
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?
pre-tRNAHis + H2O
mature tRNAHis + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of fly substrate, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNALeu + H2O
tRNALeu + 5' leader of tRNA
show the reaction diagram
pre-tRNAMet_ini + H2O
mature tRNAMet_ini + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of fly initiator methionine tRNA, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNAPhe + H2O
?
show the reaction diagram
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?
pre-tRNAPhe + H2O
tRNAPhe + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNATyr + H2O
mature tRNATyr + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of human substrate, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNATyr + H2O
mature tRNATyr + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNATyr + H2O
tRNATyr + 5' leader of tRNA
show the reaction diagram
pre-tRNATyr + H2O
tRNATyr + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNATyr precursor + H2O
mature tRNATyr + 5'-terminal oligonucleotide
show the reaction diagram
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?
precursor tRNA + H2O
77- and 35-RNA fragments
show the reaction diagram
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ribonuclease P is the endonuclease that removes the leader fragments from the 5'-ends of precursor tRNAs
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?
precursor tRNA + H2O
?
show the reaction diagram
precursor tRNA Gly + H2O
mature tRNA Gly + 5'-GGAUUUUCCCUUUC
show the reaction diagram
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5' flank with homogeneous 3' end, CCAGUC-3'
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?
precursors to 4.5 S RNA + H2O
?
show the reaction diagram
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?
ptRNATyr + H2O
?
show the reaction diagram
ssRNA oligonucleotide + H2O
5'-phospho-3'-hydroxy-ribonucleotides
show the reaction diagram
SupS1 precursor + H2O
?
show the reaction diagram
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?
syntaxin18 mRNA + H2O
?
show the reaction diagram
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?
tmRNA + H2O
?
show the reaction diagram
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?
tmRNA precursor + H2O
mature tmRNA + 5'-oligonucleotide
show the reaction diagram
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RNA processing
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?
tRNA precursor + H2O
mature tRNA + 5'-oligonucleotide
show the reaction diagram
tRNA precursor + H2O
mature tRNA + 5'-terminal oligonucleotide
show the reaction diagram
tRNA-like pseudoknotted structures in viral RNA + H2O
processed RNA + ?
show the reaction diagram
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tRNA-like pseudoknotted structures in viral RNA
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?
tRNAAsp precursor + H2O
mature tRNAAsp + 5'-terminal oligonucleotide
show the reaction diagram
tRNALeu5 + H2O
?
show the reaction diagram
tRNAPhe (A+1) precursor + H2O
mature tRNAPhe + 5'-terminal oligonucleotide
show the reaction diagram
tRNAPhe (G+1) precursor + H2O
mature tRNAPhe + 5'-terminal oligonucleotide
show the reaction diagram
tRNAPhe precursor + H2O
mature tRNAPhe + 5'-terminal oligonucleotide
show the reaction diagram
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maize tRNA substrate, structure
cleavage site determination
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?
tRNATyr precursor + H2O
mature tRNATyr + 5'-oligonucleotide
show the reaction diagram
tRNATyr precursor + H2O
mature tRNATyr + 5'-terminal oligonucleotide
show the reaction diagram
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?
tRNATyrUAG precursor + H2O
?
show the reaction diagram
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RNase P cleavage of this substrate generates a 5' matured tRNA with a 7 base pair amino acceptor stem
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?
additional information
?
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