Application | Comment | Organism |
---|---|---|
analysis | coupling DNA micro-arrays with conditional mutants of RNase P in yeast is a powerful approach to conduct genomewide searches and identify new substrates (e.g., polycistronic mRNAs, snoRNAs) | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
nucleus | - |
Homo sapiens | 5634 | - |
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity purification | Homo sapiens |
affinity purified | Saccharomyces cerevisiae |
partially purified | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | yeast RNase P may process antisense RNAs from genes encoding ribosomal proteins | Saccharomyces cerevisiae | ? | - |
? | |
pre-tRNA + H2O | - |
Homo sapiens | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | the base at N-1 in the pre-tRNA interacts with A248 in the RNase P RNA | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MRPP1 | - |
Homo sapiens |
MRPP2 | - |
Homo sapiens |
MRPP3 | - |
Homo sapiens |
POP1 | - |
Homo sapiens |
Pop5 | - |
Homo sapiens |
Pop5 | - |
Methanothermobacter thermautotrophicus |
ribonuclease P | - |
Escherichia coli |
ribonuclease P | - |
Homo sapiens |
ribonuclease P | - |
Saccharomyces cerevisiae |
ribonuclease P | - |
Methanothermobacter thermautotrophicus |
RNase P | - |
Escherichia coli |
RNase P | - |
Homo sapiens |
RNase P | - |
Saccharomyces cerevisiae |
RNase P | - |
Methanothermobacter thermautotrophicus |
RPP14 | - |
Homo sapiens |
RPP14 | - |
Saccharomyces cerevisiae |
Rpp20 | - |
Homo sapiens |
Rpp20 | - |
Saccharomyces cerevisiae |
Rpp21 | - |
Homo sapiens |
Rpp21 | - |
Saccharomyces cerevisiae |
Rpp21 | - |
Methanothermobacter thermautotrophicus |
Rpp25 | - |
Homo sapiens |
Rpp25 | - |
Saccharomyces cerevisiae |
Rpp29 | - |
Homo sapiens |
Rpp29 | - |
Saccharomyces cerevisiae |
Rpp29 | - |
Methanothermobacter thermautotrophicus |
Rpp30 | - |
Homo sapiens |
Rpp30 | - |
Saccharomyces cerevisiae |
Rpp30 | - |
Methanothermobacter thermautotrophicus |
Rpp38 | - |
Homo sapiens |
Rpp38 | - |
Saccharomyces cerevisiae |
RPP40 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | native RNase P turnover at steady state is limited by product release | Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | down-regulation of a single RNase P protein in cultured human cells results in a concomitant decrease of up to four other RNase P proteins (but not the RNase P RNA), likely due to transcriptional repression | down |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of RNase P results in decreased transcription of several non-coding RNAs in a cell cycle-dependent fashion | Homo sapiens |
physiological function | native nuclear RNase P has an RNase P RNA plus nine RNase P proteins. All subunits are essential for RNase P activity and cell viability. Only the nuclear-encoded RPM2 is known and shown genetically to be required for mitochondrial RNase P activity | Saccharomyces cerevisiae |
physiological function | native nuclear RNase P has an RNase P RNA plus ten RNase P proteins. The protein-only mitochondrial RNase P is composed of three proteins (MRPP1-MRPP3). MRPP1, which methylates G9 of tRNAs, may be responsible for substrate recognition. RNase P RNA is weakly active without RNase P proteins, some activity is present when reconstituted with RPP21 and RPP29 | Homo sapiens |
physiological function | the holoenzyme consists of a single RNase P RNA associated with RNase P protein subunits | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | a native precursor form of yeast RNase P (RNase P RNA + 7 RNase P proteins, without POP3/RPP38 and Rpr2p/RPP21) displays an identical steady-state rate as the mature form (RNase P RNA + 9 RNase P proteins) | Saccharomyces cerevisiae |