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Literature summary for 3.1.26.5 extracted from
- Targeting mRNAs by engineered sequence-specific RNase P ribozymes (2012), Methods Mol. Biol., 848, 357-368.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | RNase P of Escherichia coli contains a catalytic RNA subunit (M1 RNA) that can be engineered to cleave tRNA-like substrates and other target RNAs, including specific mRNAs. The enzyme can be expressed in infected human U373MG cells or fibroblasts, or in murine PA317 cells, and therein be used for inhibition of targeting and cleavage of host RNA by Human cytomegalovirus enzyme, HCMV strain AD169, through blocking substrate mRNA expression, methods of using engineered RNase P catalytic RNA for in vitro and in vivo in trans-cleavage of target viral mRNA, overview. customized M1GS RNA and full-length RNase P are effective in cleaving both viral and cellular mRNAs and blocking their expression in cultured cells | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| ribonucleoprotein | - |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | method development for the engineered catalytic RNA subunit of Escherichia coli RNase P to cleave tRNA-like substrates and other target RNAs, including specific mRNAs, detailed overview | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase P | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.4 | assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | RNase P-mediated inhibition of gene expression represents a novel and promising nucleic acid-based gene interference strategy for specific inhibition of target mRNA, overview | Escherichia coli |
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