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Literature summary for 3.1.26.5 extracted from
- RNase P enzymes: divergent scaffolds for a conserved biological reaction (2013), RNA Biol., 10, 909-914.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure analysis of PRORP enzyme in complex with tRNA | Ostreococcus tauri |
| crystal structure analysis of PRORP enzyme in complex with tRNA | Arabidopsis thaliana |
| crystal structure analysis of RNase P enzyme in complex with tRNA, PDB ID 3Q1R | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Trypanosoma brucei proteinaceous enzyme PRORP1 can substitute for yeast nuclear RNase P in vivo | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bacillus subtilis | |
| additional information | - |
additional information | kinetics | Saccharomyces cerevisiae | |
| additional information | - |
additional information | kinetics | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Ostreococcus tauri | 9507 | - |
| chloroplast | PRORP1 | Arabidopsis thaliana | 9507 | - |
| mitochondrion | - |
Ostreococcus tauri | 5739 | - |
| mitochondrion | PRORP1 | Arabidopsis thaliana | 5739 | - |
| mitochondrion | isozyme PRORP2 | Trypanosoma brucei | 5739 | - |
| additional information | in plants, the localization of PRORP1 in chloroplast and mitochondria differs from PRORP2 and 3 in the nucleus | Arabidopsis thaliana | - |
- |
| additional information | the chloroplast and mitochondrial genomes of Ostreococcus tauri encode distinct individual RNase P RNA genes and the nucleus encodes both a bacterial-like RNase P protein component, and a proteinaceous RNase P enzyme | Ostreococcus tauri | - |
- |
| nucleus | - |
Homo sapiens | 5634 | - |
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
| nucleus | - |
Ostreococcus tauri | 5634 | - |
| nucleus | isozyme PRORP1 | Trypanosoma brucei | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus subtilis | the natural substrate is precursor tRNA | ? | - |
? |  |
| additional information | Homo sapiens | the natural substrate is precursor tRNA | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | the natural substrate is precursor tRNA | ? | - |
? | |
| additional information | Thermotoga maritima | the natural substrate is precursor tRNA | ? | - |
? | |
| additional information | Ostreococcus tauri | the natural substrate is precursor tRNA | ? | - |
? | |
| additional information | Arabidopsis thaliana | the natural substrate is precursor tRNA | ? | - |
? | |
| additional information | Trypanosoma brucei | the natural substrate is precursor tRNA. Proteinaceous PRORP1 catalyzes all of the other noncanonical, yet vital functions of nuclear yeast RNase P, which may include processing of non-canonical RNAs | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q66GI4 | - |
- |
| Bacillus subtilis | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Ostreococcus tauri | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Thermotoga maritima | - |
- |
- |
| Trypanosoma brucei | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no ribonucleoprotein | - |
Arabidopsis thaliana |
| no ribonucleoprotein | PRORP1 and 2 | Trypanosoma brucei |
| no ribonucleoprotein | proteinaceous RNase P and chloroplast and mitochondrial enzymes, overview | Ostreococcus tauri |
| ribonucleoprotein | bacterial RNase Ps consist of one RNA and one protein | Bacillus subtilis |
| ribonucleoprotein | bacterial RNase Ps consist of one RNA and one protein | Thermotoga maritima |
| ribonucleoprotein | bacterial-like RNase P and chloroplast and mitochondrial enzymes, overview | Ostreococcus tauri |
| ribonucleoprotein | most eukaryal nuclear RNase Ps are RNP based, which contain one RNA and at least nine proteins | Homo sapiens |
| ribonucleoprotein | most eukaryal nuclear RNase Ps are RNP based, which contain one RNA and at least nine proteins | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the natural substrate is precursor tRNA | Bacillus subtilis | ? | - |
? | |
| additional information | the natural substrate is precursor tRNA | Homo sapiens | ? | - |
? | |
| additional information | the natural substrate is precursor tRNA | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | the natural substrate is precursor tRNA | Thermotoga maritima | ? | - |
? | |
| additional information | the natural substrate is precursor tRNA | Ostreococcus tauri | ? | - |
? | |
| additional information | the natural substrate is precursor tRNA | Arabidopsis thaliana | ? | - |
? | |
| additional information | the natural substrate is precursor tRNA. Proteinaceous PRORP1 catalyzes all of the other noncanonical, yet vital functions of nuclear yeast RNase P, which may include processing of non-canonical RNAs | Trypanosoma brucei | ? | - |
? | |
| additional information | the enzyme PRORP1 cleaves pre-tRNAs substrates lacking an anticodon arm, the recognition mechanism involves the D-TpsiC loops in tRNA, overview. It is also active on Thermus thermophilus pre-tRNAGly | Arabidopsis thaliana | ? | - |
? | |
| additional information | the enzyme PRORP1 cleaves pre-tRNAs substrates lacking an anticodon arm,n recognition mechanism involves the D-TpsiC loops in tRNA, overview | Thermotoga maritima | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PRORP | - |
Ostreococcus tauri |
| PRORP | - |
Arabidopsis thaliana |
| PRORP1 | - |
Trypanosoma brucei |
| PRORP1 | - |
Arabidopsis thaliana |
| PRORP2 | - |
Trypanosoma brucei |
| proteinaceous RNase P | - |
Trypanosoma brucei |
| proteinaceous RNase P | - |
Ostreococcus tauri |
| proteinaceous RNase P | - |
Arabidopsis thaliana |
| RNase P | - |
Bacillus subtilis |
| RNase P | - |
Homo sapiens |
| RNase P | - |
Saccharomyces cerevisiae |
| RNase P | - |
Trypanosoma brucei |
| RNase P | - |
Thermotoga maritima |
| RNase P | - |
Ostreococcus tauri |
| RNase P | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
| 37 | - |
assay at | Saccharomyces cerevisiae |
| 37 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8 | assay at | Bacillus subtilis |
| 7.8 | 8 | assay at | Saccharomyces cerevisiae |
| 7.8 | 8 | assay at | Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is conserved in all domains of life. The composition of RNase P varies from bacteria to archaea and eukarya, evolutionary enzyme spread, overview | Bacillus subtilis |
| evolution | the enzyme is conserved in all domains of life. The composition of RNase P varies from bacteria to archaea and eukarya, evolutionary enzyme spread, overview | Homo sapiens |
| evolution | the enzyme is conserved in all domains of life. The composition of RNase P varies from bacteria to archaea and eukarya, evolutionary enzyme spread, overview | Saccharomyces cerevisiae |
| evolution | the enzyme is conserved in all domains of life. The composition of RNase P varies from bacteria to archaea and eukarya, evolutionary enzyme spread, overview | Thermotoga maritima |
| evolution | the enzyme is conserved in all domains of life. The composition of RNase P varies from bacteria to archaea and eukarya, evolutionary enzyme spread, overview | Arabidopsis thaliana |
| evolution | the enzyme is conserved in all domains of life. The composition of RNase P varies from bacteria to archaea and eukarya, evolutionary enzyme spread, overview. The chloroplast and mitochondrial genomes of Ostreococcus tauri encode distinct individual RNase P RNA genes and the nucleus encodes both a bacterial-like RNase P protein component, and a proteinaceous RNase P enzyme | Ostreococcus tauri |
| evolution | the enzyme is conserved in all domains of life. The composition of RNase P varies from bacteria to archaea and eukarya, evolutionary enzyme spread, overview. The protozoan Trypanosoma brucei harbors 2 PRORP isoforms, both of which have 5' pre-tRNA processing activity in vitro. One isoform (PRORP1) localizes to the nucleus and the second (PRORP2) to the mitochondrion | Trypanosoma brucei |
| additional information | Arabidopsis thaliana contains a protein only form of RNase P, modeling of PRORP-tRNA interaction | Arabidopsis thaliana |
| additional information | modeling of RNP-based RNase P | Bacillus subtilis |
| additional information | modeling of RNP-based RNase P | Homo sapiens |
| additional information | modeling of RNP-based RNase P | Saccharomyces cerevisiae |
| additional information | modeling of RNP-based RNase P | Trypanosoma brucei |
| additional information | modeling of RNP-based RNase P | Thermotoga maritima |
| additional information | the organellar RNase P RNAs are expressed in vivo, however under in vitro conditions, catalysis of pre-tRNA cleavage is not observed even when associated with the nuclear encoded bacterial-like protein. Modeling of PRORP-tRNA interaction and RNP-based RNase P | Ostreococcus tauri |
| physiological function | the enzyme catalyzes the maturation of the 5' end of precursor-tRNAs | Bacillus subtilis |
| physiological function | the enzyme catalyzes the maturation of the 5' end of precursor-tRNAs | Homo sapiens |
| physiological function | the enzyme catalyzes the maturation of the 5' end of precursor-tRNAs | Saccharomyces cerevisiae |
| physiological function | the enzyme catalyzes the maturation of the 5' end of precursor-tRNAs | Thermotoga maritima |
| physiological function | the enzyme catalyzes the maturation of the 5' end of precursor-tRNAs | Ostreococcus tauri |
| physiological function | the enzyme catalyzes the maturation of the 5' end of precursor-tRNAs | Arabidopsis thaliana |
| physiological function | the enzyme catalyzes the maturation of the 5' end of precursor-tRNAs. Trypanosoma brucei proteinaceous enzyme PRORP1 can substitute for yeast nuclear RNase P in vivo. Proteinaceous PRORP1 catalyzes all of the other noncanonical, yet vital functions of nuclear yeast RNase P, which may include processing of non-canonical RNAs | Trypanosoma brucei |
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Release 2023.1 (January 2023)
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