Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | binding and catalysis of J5/15 and N(-1) mutants, overview, mutation of the A248 nucleotide results in defects in thermodynamics and in miscleavage of substrates containing 2'deoxy modification at N(-1), compensatory mutation of N(-1) restore correct cleavage activity in reaction with both, the RNA subunit alone and the holoenzyme, influence of nucleotide at position N(-2) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | - |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, conserved residues in J5/15 are responsible for substrate affinity and specificity of the ribozyme, nucleotide base N(-1) is involved in substrate recognition in bacteria and archea | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, the 5'leader of the pre-tRNA substrate is recognized by the active site of the enzyme via interaction of N(-1) substrate nucleotide with A248 of the ribozyme, preference for U at position N(-1) | Escherichia coli | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme is composed of a large RNA subunit of about 400 nucleotides and a smaller protein subunit | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Escherichia coli |
tRNA processing enzyme | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |