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Literature summary for 3.1.26.5 extracted from

  • Mohanty, B.K.; Kushner, S.R.
    Processing of the Escherichia coli leuX tRNA transcript, encoding tRNA(Leu5), requires either the 3-->5 exoribonuclease polynucleotide phosphorylase or RNase P to remove the Rho-independent transcription terminator (2010), Nucleic Acids Res., 38, 597-607.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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Escherichia coli 5739
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Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Escherichia coli MG1693
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNALeu5 + H2O RNase P, the endonuclease responsible for generating mature 5' termini, also plays a role in the 3'-end processing of leuX. It removes the terminator from ca. 10% of primary transcripts by cleaving 4-7 nt downstream of the CCA determinant, generating substrates for RNase II, which removes an additional 3-4 nt Escherichia coli ?
-
?
tRNALeu5 + H2O RNase P, the endonuclease responsible for generating mature 5' termini, also plays a role in the 3'-end processing of leuX. It removes the terminator from ca. 10% of primary transcripts by cleaving 4-7 nt downstream of the CCA determinant, generating substrates for RNase II, which removes an additional 3-4 nt Escherichia coli MG1693 ?
-
?

Synonyms

Synonyms Comment Organism
ribonuclease P
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Escherichia coli
RNase P
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Escherichia coli

General Information

General Information Comment Organism
malfunction in a strain carrying the rnpA49 allele, encoding temperature sensitive RNase P, thermal inactivation of RNase P leads to ca. 60% reduction in relative quantities of the mature tRNA, although there is no change the relative quantities of the primary transcripts. Inactivation of both RNase P and RNase E leads to disappearance of the majority of the heterogeneous pre-tRNA precursor species except for the species containing the intact 5'-end and a processed 3'-end. In the absence of RNase P (both rnpA49 and rnpA49 rne-1) ca. 77% (36/49) of the transcripts have immature 3' termini containing 1-3 nt downstream of the CCA Escherichia coli