Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Escherichia coli | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli MG1693 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNALeu5 + H2O | RNase P, the endonuclease responsible for generating mature 5' termini, also plays a role in the 3'-end processing of leuX. It removes the terminator from ca. 10% of primary transcripts by cleaving 4-7 nt downstream of the CCA determinant, generating substrates for RNase II, which removes an additional 3-4 nt | Escherichia coli | ? | - |
? | |
tRNALeu5 + H2O | RNase P, the endonuclease responsible for generating mature 5' termini, also plays a role in the 3'-end processing of leuX. It removes the terminator from ca. 10% of primary transcripts by cleaving 4-7 nt downstream of the CCA determinant, generating substrates for RNase II, which removes an additional 3-4 nt | Escherichia coli MG1693 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease P | - |
Escherichia coli |
RNase P | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | in a strain carrying the rnpA49 allele, encoding temperature sensitive RNase P, thermal inactivation of RNase P leads to ca. 60% reduction in relative quantities of the mature tRNA, although there is no change the relative quantities of the primary transcripts. Inactivation of both RNase P and RNase E leads to disappearance of the majority of the heterogeneous pre-tRNA precursor species except for the species containing the intact 5'-end and a processed 3'-end. In the absence of RNase P (both rnpA49 and rnpA49 rne-1) ca. 77% (36/49) of the transcripts have immature 3' termini containing 1-3 nt downstream of the CCA | Escherichia coli |