Literature summary for 3.1.26.5 extracted from

Suwa, S.; Nagai, Y.; Fujimoto, A.; Kikuchi, Y.; Tanaka, T.
Analysis on substrate specificity of Escherichia coli ribonuclease P using shape variants of pre-tRNA: proposal of subsites model for substrate shape recognition (2009), J. Biochem., 145, 151-160.

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Cloned(Commentary)

Cloned (Comment)	Organism
the RNase P ribozyme subunit is expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential for activity, the ribozyme prefers the wild type pre-tRNA (A7) substrate at low Mg2+	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pre-tRNA + H2O	the RNase P ribozyme and the holoenzyme demonstrate strict shape specificity towards these RNAs, but the holoenzyme cannot distinguish a pre-tRNA from a hairpin RNA mimicking the top half of a pre-tRNA, both the ribozyme and the holoenzyme prefer longer acceptor-stem RNAs to shorter	Escherichia coli	tRNA + 5'-oligoribonucleotide	-	?

Synonyms

Synonyms	Comment	Organism
RNase P	the bacterial RNase P comprises an RNA subunit and a small protein subunit	Escherichia coli

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Release 2023.1 (January 2023)