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Literature summary for 3.1.26.5 extracted from
- Partial purification and characterization of RNase P from Dictyostelium discoideum (1995), Eur. J. Biochem., 228, 976-980.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| micrococcal nuclease | - |
Dictyostelium discoideum | |
| proteinase K | - |
Dictyostelium discoideum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00024 | - |
pre-tRNA supS1 tRNASer | - |
Dictyostelium discoideum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | optimal activity in presence of 5 mM KCl or 10 mM NH4Cl | Dictyostelium discoideum |  |
| Mg2+ | optimal activity in presence of 5 mM MgCl2 | Dictyostelium discoideum | |
| NH4+ | optimal activity in presence of 5 mM KCl or 10 mM NH4Cl | Dictyostelium discoideum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Dictyostelium discoideum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dictyostelium discoideum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pre-tRNA precursor + H2O | cleaves the precursor to Schizosaccharomyces pombe suppressor tRNASer at the same site as Schizosaccharomyces pombe RNase P, producing the mature 5' end of tRNASer | Dictyostelium discoideum | tRNA + 5'-oligoribonucleotide | - |
? | |
| pre-tRNA supS1 tRNASer + H2O | - |
Dictyostelium discoideum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Dictyostelium discoideum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 42 | 25°C: about 60% of maximal activity, 42°C: about 25% of maximal activity | Dictyostelium discoideum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Dictyostelium discoideum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.2 | pH 7.0: about 30% of maximal activity, pH 8.2: about 45% of maximal activity | Dictyostelium discoideum |
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