Cloned (Comment) | Organism |
---|---|
expression of the protein and RNA components of mycobacterial RNase P in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H67A | spectrum of mutant is moderately altered at 37°C, indicative of subtle structural changes arising due to the mutation. The H67A protein shows significant loss in secondary structure at 45°C, which further enhances at 55°C. The loss of structure with increase in temperature is relatively less in the case of wild-type and H67N proteins. kcat/Km-value is 1.9fold lower than the value for the wild-type enzyme | Mycobacterium tuberculosis |
H67N | spectrum of mutant is moderately altered at 37°C, indicative of subtle structural changes arising due to the mutation. The H67A protein shows significant loss in secondary structure at 45°C, which further enhances at 55°C. The loss of structure with increase in temperature is relatively less in the case of wild-type and H67N proteins. kcat/Km-value is 1.4fold lower than the value for the wild-type enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the RNA component alone shows activity on pre-tRNAala substrate at high magnesium concentrations (50 mM). The RNA and protein components associate together to manifest catalytic activity at low magnesium concentrations (20 mM) | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WGZ3 | the enzyme consists of a catalytic RNA component (M1 or rnpB) and a protein subunit rnpA (P9WGZ3) | - |
Mycobacterium tuberculosis H37Rv | P9WGZ3 | the enzyme consists of a catalytic RNA component (M1 or rnpB) and a protein subunit rnpA (P9WGZ3) | - |
Purification (Comment) | Organism |
---|---|
purification of the recombinant protein and RNA components and reconstitution of the holoenzyme in vitro | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA-Ala + H2O | the RNA component alone shows activity on pre-tRNAala substrate at high magnesium concentrations (50 mM). The RNA and protein components associate together to manifest catalytic activity at low magnesium concentrations (20 mM). The histidine 67 in the RNR motif of RNase P protein component is important for the catalytic activity and stability of the enzyme | Mycobacterium tuberculosis | mature tRNA-Ala + 5'-terminal oligonucleotide | - |
? | |
pre-tRNA-Ala + H2O | the RNA component alone shows activity on pre-tRNAala substrate at high magnesium concentrations (50 mM). The RNA and protein components associate together to manifest catalytic activity at low magnesium concentrations (20 mM). The histidine 67 in the RNR motif of RNase P protein component is important for the catalytic activity and stability of the enzyme | Mycobacterium tuberculosis H37Rv | mature tRNA-Ala + 5'-terminal oligonucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | all bacterial RNase Ps have one RNA and one protein component. A conserved RNR motif in bacterial RNase P protein components is involved in their interaction with the RNA component | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | 25°C: about 90% of maximal activity, 45°C: about 65% of maximal activity | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the 5' end processing of pre-tRNAs | Mycobacterium tuberculosis |