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Literature summary for 3.1.26.5 extracted from

  • Agrawal, A.; Mohanty, B.K.; Kushner, S.R.
    Processing of the seven valine tRNAs in Escherichia coli involves novel features of RNase P (2015), Nucleic Acids Res., 42, 11166-11179.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Protein Variants

Protein Variants Comment Organism
additional information construction of the enzyme-deficient rnpA49 rph-1 mutant strain. Increased levels of the M1 RNA, the enzyme's rNA subunit, partially complement the rnpA49 allele at 42°C. Simultaneous expression of tRNAval(GAC) and tRNAval(UAC) does not suppress the temperature sensitivity of the rnpA49 strain. In contrast, overproduction of the rnpB gene leads to a small improvement in the growth of the rnpA49 rph-1 strain at 42°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the mechanism by which the enzyme processes the valU and lysT polycistronic transcripts (valV valW, valU valX, valY lysY and lysT valT lysW valZ lysY lysZ lysQ) involves initiation of processing by first endonucleolytically removing the Rho-independent transcription terminators from the primary valU and lysT transcripts. Subsequently, the enzyme proceeds in the 3' -> 5' direction generating one pre-tRNA at a time. Identification of cleavage sites using RNA circularization, overview ?
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 ?
additional information Escherichia coli MG1693 the mechanism by which the enzyme processes the valU and lysT polycistronic transcripts (valV valW, valU valX, valY lysY and lysT valT lysW valZ lysY lysZ lysQ) involves initiation of processing by first endonucleolytically removing the Rho-independent transcription terminators from the primary valU and lysT transcripts. Subsequently, the enzyme proceeds in the 3' -> 5' direction generating one pre-tRNA at a time. Identification of cleavage sites using RNA circularization, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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 gene rnpA encodes C5 protein, gene mpB encodes the catalytic M1 RNA subunit
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Escherichia coli MG1693
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 gene rnpA encodes C5 protein, gene mpB encodes the catalytic M1 RNA subunit
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Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein the essential enzyme consists of the C5 protein and the catalytic M1 RNA subunits Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the mechanism by which the enzyme processes the valU and lysT polycistronic transcripts (valV valW, valU valX, valY lysY and lysT valT lysW valZ lysY lysZ lysQ) involves initiation of processing by first endonucleolytically removing the Rho-independent transcription terminators from the primary valU and lysT transcripts. Subsequently, the enzyme proceeds in the 3' -> 5' direction generating one pre-tRNA at a time. Identification of cleavage sites using RNA circularization, overview Escherichia coli ?
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 ?
additional information the mechanism by which the enzyme processes the valU and lysT polycistronic transcripts (valV valW, valU valX, valY lysY and lysT valT lysW valZ lysY lysZ lysQ) involves initiation of processing by first endonucleolytically removing the Rho-independent transcription terminators from the primary valU and lysT transcripts. Subsequently, the enzyme proceeds in the 3' -> 5' direction generating one pre-tRNA at a time. Identification of cleavage sites using RNA circularization, overview Escherichia coli MG1693 ?
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 ?

Subunits

Subunits Comment Organism
More the essential enzyme consists of the C5 protein and the catalytic M1 RNA subunits Escherichia coli

Synonyms

Synonyms Comment Organism
RNase P
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 Escherichia coli

General Information

General Information Comment Organism
malfunction absence of the enzyme in mutant rnpA49 rph-1 strain results in accumulation of unprocessed large tRNA transcripts and a 4fold decrease in mature species Escherichia coli
physiological function the enzyme is required for the initial separation of all seven valine tRNAs from three distinct polycistronic transcripts, the processing of the seven valine tRNAs in Escherichia coli demands special features of the enzyme. Processing of the valU polycistronic transcript is completely dependent on RNase P. Processing of the lysT polycistronic operon requires RNase P but is stimulated by RNase E, EC 3.1.26.12 Escherichia coli