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Literature summary for 3.1.26.5 extracted from

  • Mann, H.; Ben-asouli, Y.; Schein, A.; Moussa, S.; Jarrous, N.
    Eukaryotic RNase P: role of RNA and protein subunits of a primordial catalytic ribonucleoprotein in RNA-based catalysis (2003), Mol. Cell, 12, 925-935.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged protein subunits Rpp29, Rpp21, and RNA subunit H1 in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaCl inhibition of the reconstituted mini-enzyme at 0.4 M NaCl Homo sapiens
Zn2+ results in misscleavage of te substrate at 1-5 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal cleavage at 20 mM for the reconstituted mini-enzyme, reduced activity at 40-100 mM, the wild-type enzyme shows no activity at 100 mM Homo sapiens
additional information Ca2+ and Mn2+ cannot substitute for Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNA precursor + H2O Homo sapiens 5'-leader sequence tRNA processing, essential enzyme mature tRNA + 5'-oligonucleotide
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein and RNA subunits H1, Rpp21, and Rpp29 from Escherichia coli Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of a functional mini-enzyme from protein subunits Rpp21 and Rpp29 with the RNA subunit H1, all 3 coponents are essential for reconstitution Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information protein subunits Rpp21 and Rpp29 and RNA subunit H1 are sufficient for effective substrate cleavage, thereby the protein subunits facilitate catalytic activity of RNA subunit H1 which requires a phylogenetically conserved pseudoknot-structure for function, protein subunit Rpp29 formsa catalytic complex with M1 RNA from Escherichia coli Homo sapiens ?
-
?
tRNA precursor + H2O 5'-leader sequence tRNA processing, essential enzyme Homo sapiens mature tRNA + 5'-oligonucleotide
-
?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, reconstituted mini-enzyme and wild-type enzyme, both cleave at positions G28-G29 Homo sapiens mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product, reconstituted mini-enzyme and wild-type enzyme ?
tRNATyr precursor + H2O cleavage of 5'-terminal oligonucleotide, reconstituted mini-enzyme and wild-type enzyme, both cleave at positions G28-G29 Homo sapiens mature tRNATyr + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product, reconstituted mini-enzyme and wild-type enzyme ?

Synonyms

Synonyms Comment Organism
RNase P
-
Homo sapiens