Cloned (Comment) | Organism |
---|---|
expression of His-tagged protein subunits Rpp29, Rpp21, and RNA subunit H1 in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | inhibition of the reconstituted mini-enzyme at 0.4 M NaCl | Homo sapiens | |
Zn2+ | results in misscleavage of te substrate at 1-5 mM | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal cleavage at 20 mM for the reconstituted mini-enzyme, reduced activity at 40-100 mM, the wild-type enzyme shows no activity at 100 mM | Homo sapiens | |
additional information | Ca2+ and Mn2+ cannot substitute for Mg2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA precursor + H2O | Homo sapiens | 5'-leader sequence tRNA processing, essential enzyme | mature tRNA + 5'-oligonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein and RNA subunits H1, Rpp21, and Rpp29 from Escherichia coli | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
reconstitution of a functional mini-enzyme from protein subunits Rpp21 and Rpp29 with the RNA subunit H1, all 3 coponents are essential for reconstitution | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | protein subunits Rpp21 and Rpp29 and RNA subunit H1 are sufficient for effective substrate cleavage, thereby the protein subunits facilitate catalytic activity of RNA subunit H1 which requires a phylogenetically conserved pseudoknot-structure for function, protein subunit Rpp29 formsa catalytic complex with M1 RNA from Escherichia coli | Homo sapiens | ? | - |
? | |
tRNA precursor + H2O | 5'-leader sequence tRNA processing, essential enzyme | Homo sapiens | mature tRNA + 5'-oligonucleotide | - |
? | |
tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, reconstituted mini-enzyme and wild-type enzyme, both cleave at positions G28-G29 | Homo sapiens | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product, reconstituted mini-enzyme and wild-type enzyme | ? | |
tRNATyr precursor + H2O | cleavage of 5'-terminal oligonucleotide, reconstituted mini-enzyme and wild-type enzyme, both cleave at positions G28-G29 | Homo sapiens | mature tRNATyr + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product, reconstituted mini-enzyme and wild-type enzyme | ? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Homo sapiens |