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Literature summary for 3.1.26.5 extracted from

  • Guerrier-Takada, C.; Haydock, K.; Allen, L.; Altman, S.
    Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coli (1986), Biochemistry, 25, 1509-1515.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-methyl-2,4-pentanediol enhances cleavage reaction of the M1 RNA subunit alone Escherichia coli
Polyethylene glycol enhances cleavage reaction of the M1 RNA subunit alone Escherichia coli
spermidine M1 RNA subunit can catalyze the cleavage of tRNA precursor molecules in the presence of 5 mM spermine or spermidine Escherichia coli
spermine M1 RNA subunit can catalyze the cleavage of tRNA precursor molecules in the presence of 5 mM spermine or spermidine Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli
Mg2+ the only metal ion that can act as cofactor for activity of M1 RNA Escherichia coli
Mg2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli
Mn2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli
NH4+ stimulates activity of M1 RNA Escherichia coli
Sr2+ Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA precursor + H2O
-
Escherichia coli tRNA + 5'-oligoribonucleotide
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 9.5
-
Escherichia coli