Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-methyl-2,4-pentanediol | enhances cleavage reaction of the M1 RNA subunit alone | Escherichia coli | |
Polyethylene glycol | enhances cleavage reaction of the M1 RNA subunit alone | Escherichia coli | |
spermidine | M1 RNA subunit can catalyze the cleavage of tRNA precursor molecules in the presence of 5 mM spermine or spermidine | Escherichia coli | |
spermine | M1 RNA subunit can catalyze the cleavage of tRNA precursor molecules in the presence of 5 mM spermine or spermidine | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations | Escherichia coli | |
Mg2+ | the only metal ion that can act as cofactor for activity of M1 RNA | Escherichia coli | |
Mg2+ | Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations | Escherichia coli | |
Mn2+ | Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations | Escherichia coli | |
NH4+ | stimulates activity of M1 RNA | Escherichia coli | |
Sr2+ | Mg2+, Ca2+, Sr2+, and, to a lesser extent, Mn2+ can perform the electrostatic shielding function and preserve the structural properties of the two RNA molecules necessary to keep the substrate and enzyme in appropriate conformations | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA precursor + H2O | - |
Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | - |
Escherichia coli |