Application | Comment | Organism |
---|---|---|
medicine | construction of external guide sequences (EGSs) from a variant to target the mRNA encoding herpes simplex virus 1 (HSV-1) major transcription regulator ICP4, which is essential for the expression of viral early and late genes and viral growth. The EGS variant induces human RNase P cleavage of ICP4 mRNA sequence 60times better than the EGS generated from a natural pre-tRNA. A decrease of about 97% and 75% in the level of ICP4 gene expression and an inhibition of about 7,000- and 500-fold in viral growth are observed in HSV infected cells expressing the variant and the pre-tRNA-derived EGS, respectively. The study shows that engineered external guide sequences (EGSs) can inhibit HSV-1 gene expression and viral growth. The results demonstrate the potential for engineered EGS RNAs to be developed and used as anti-HSV therapeutics | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q99575 AND O95707 AND Q969H6 AND O75817 AND O95059 AND Q9H633 AND Q9BUL9 AND P78346 AND P78345 AND O75818 | human RNase P consists of a catalytic RNA moiety and about 10 protein subunits; POP1 (Q99575), POP4 (O95707), POP5 (Q969H6), POP7 (O75817), RPP14 (O95059), RPP21 (Q9H633), RPP25 (Q9BUL9), RPP30 (P78346), RPP38 (P78345) and RPP40 (O75818) | - |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Homo sapiens |