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Literature summary for 3.1.26.5 extracted from
- Ribonuclease P: an enzyme with a catalytic RNA subunit (1989), Adv. Enzymol. Relat. Areas Mol. Biol., 62, 1-36.
General Stability
| General Stability | Organism |
|---|---|
| Ca2+, Sr2+ and Mg2+ maintain the structure of the enzyme in solution | Escherichia coli |
| spermidine and spermine maintain the structure of the enzyme in solution | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
tRNATyr | - |
Escherichia coli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Haloferax volcanii | |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | required | Homo sapiens | |
| Mg2+ | can substitute for the C5 protein as a cofactor, since M1 RNA alone can carry out the catalytic reaction in the buffer that contains more than 20 mM Mg2+ | Escherichia coli | |
| Mn2+ | - |
Homo sapiens | |
| Mn2+ | can promote catalysis | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Escherichia coli | - |
- |
- |
| Haloferax volcanii | - |
- |
- |
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| ribonucleoprotein | - |
Homo sapiens |
| ribonucleoprotein | - |
Bos taurus |
| ribonucleoprotein | the M1 RNA component is essential for the function of the enzyme | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pre-tRNA precursor + H2O | - |
Homo sapiens | tRNA + 5'-oligoribonucleotide | - |
? | |
| pre-tRNA precursor + H2O | - |
Bos taurus | tRNA + 5'-oligoribonucleotide | - |
? | |
| pre-tRNA precursor + H2O | - |
Haloferax volcanii | tRNA + 5'-oligoribonucleotide | - |
? | |
| pre-tRNA precursor + H2O | pre-tRNATyr | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
ir | |
| pre-tRNAAsp + H2O | - |
Escherichia coli | tRNAAsp + 5'-oligoribonucleotide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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