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Literature summary for 3.1.26.5 extracted from

  • Krehan, M.; Heubeck, C.; Menzel, N.; Seibel, P.; Schoen, A.
    RNase MRP RNA and RNase P activity in plants are associated with a Pop1p containing complex (2012), Nucleic Acids Res., 40, 7956-7966.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Cloned(Commentary)

Cloned (Comment) Organism
gene At2g47290, DNA and amino acid sequence determination and analysis, AtPop1 mRNA is present in multiple splicing isoforms, possibly encoding several distinct proteins, splicing patterns of AtPop1 mRNAs, overview. Recombinant expression in Escherichia coli strain Rosetta (DE3)pLysS Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Triticum aestivum 5634
-
nucleus
-
 Arabidopsis thaliana 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21500
-
 x * 21500, AtPop1 protein, two spliced exons encoding a 190 residues long protein, starting from AUG1 from gene At2G47290, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Triticum aestivum the enzyme processes the 5'-end of tRNAs ?
-
 ?
additional information Arabidopsis thaliana the enzyme processes the 5'-end of tRNAs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana L0N807 AtPop1p protein with RNase P activity
-
Triticum aestivum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain Rosetta (DE3)pLysS by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
germinated grain wheat germ S23 extract Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme processes the 5'-end of tRNAs Triticum aestivum ?
-
 ?
additional information the enzyme processes the 5'-end of tRNAs Arabidopsis thaliana ?
-
 ?
additional information substrate used in assays is Escherichia coli pre-tRNATyr Triticum aestivum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21500, AtPop1 protein, two spliced exons encoding a 190 residues long protein, starting from AUG1 from gene At2G47290, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtPop1p
-
 Arabidopsis thaliana
Pop1p
-
 Triticum aestivum
Pop1p
-
 Arabidopsis thaliana
RNase P
-
 Triticum aestivum
RNase P
-
 Arabidopsis thaliana
RNase P/MRP protein
-
 Triticum aestivum
RNase P/MRP protein
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Triticum aestivum
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Triticum aestivum
7.5
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
additional information in plants, the protein Pop1p is associated with MRP RNAs, i.e. mitochondrial RNA processing RNAs which cleave the large rRNA precursor at the A3 site, and with the catalytic subunit of enzyme RNase P, either separately or in a single large complex. Pop1p-specific antibodies precipitate RNase P activity from wheat extracts. The eukaryotic RNase P consensus sequence with CR II and CR III that are signature elements specific for RNase P RNA Triticum aestivum
additional information in plants, the protein Pop1p is associated with MRP RNAs, i.e. mitochondrial RNA processing RNAs which cleave the large rRNA precursor at the A3 site, and with the catalytic subunit of enzyme RNase P, either separately or in a single large complex. The eukaryotic RNase P consensus sequence with CR II and CR III that are signature elements specific for RNase P RNA Arabidopsis thaliana