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(4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
-
decay time to 10%: 500 sec, relative specific activity: 13%
-
-
?
(4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
-
decay time to 10%: 150 sec, relative specific activity: 1.7%
-
-
?
(4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
-
decay time to 10%: 5700 sec, relative specific activity: 421%
-
-
?
(4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
-
decay time to 10%: 400 sec, relative specific activity: 0.4%
-
-
?
(4S)-2-(6-hydroxy-1H-indol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
?
-
-
-
-
?
4'-methyl-D-luciferin + ATP + O2
?
-
-
-
?
5,5'-dimethylluciferin + ATP + O2
dehydroluciferin-adenylate + diphosphate
-
produces only red light, 6-amino and 4 hydroxy analogs tested
-
?
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ?
5,5-dimethyloxyluciferin + hv
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ?
5,5-dimethyloxyluciferin + hv + ?
-
-
-
-
?
5,5-dimethylluciferin + ATP
5,5-dimethyloxyluciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
5-methylluciferin + ATP
5-methyloxyluciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
5-methylluciferin + ATP + O2
?
-
-
-
?
5-monomethyl-luciferyl-O-adenosine monophosphate + ?
5-monomethyloxyluciferin + hv + ?
-
-
-
-
?
6'-amino-D-luciferin + ATP
? + hv
-
-
-
-
?
6'-amino-D-luciferin + ATP + O2
?
-
-
-
?
8-anilino-1-naphthalene-sulfonate + ?
?
-
-
-
?
adenosine 5'-tetraphosphate + Photinus luciferin
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
0.75% light response relative to ATP
-
?
ADP
ATP + AMP
-
-
reaction inhibited by P1,P5-di(adenosine-5'-)pentaphosphate, but independent of luciferin
?
arachidonic acid + ATP + CoA
arachidonoyl-CoA + ?
-
-
-
-
?
arachidonic acid + ATP + CoA
arachidonoyl-CoA + AMP + diphosphate
-
-
-
-
?
ATP + O2 + oxyluciferin
?
-
assay at pH 7.5, 24-27°C
-
-
?
coelenterazine disulfate + ATP + O2 + H2O
coelenteramide disulfate + AMP + CO2 + diphosphate
-
-
-
?
D-5,5-dimethylluciferyl-adenylate + O2
?
yellow-green color of luminescence
-
-
?
D-5,5-dimethylluciferyl-adenylate + O2 + ATP
?
red color of luminescence
-
-
?
D-aminoluciferin + ATP + O2
?
-
assay at 37°C. 5 min
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
D-luciferin + ATP
diphosphate + luciferyl-adenylate
-
-
-
r
D-luciferin + ATP + O2
luciferyl-adenylate
-
-
-
?
D-luciferin + ATP + O2
luciferyl-adenylate + diphosphate
-
-
-
r
D-luciferin + ATP + O2
luciferyl-adenylate + diphosphate + H2O + hv
D-luciferin + ATP + O2
luciferyl-AMP + diphosphate
-
assay at pH 7.8
-
-
r
D-luciferin + ATP + O2
oxidized D-luciferin + AMP + CO2 + diphosphate
D-luciferin + ATP + O2
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
D-luciferin + ATP + O2
oxidized luciferin + AMP + CO2 + diphosphate
-
-
-
-
?
D-luciferin + ATP + O2
oxidized luciferin + AMP + CO2 + diphosphate + hv
D-luciferin + ATP + O2
oxyluciferin + CO2 + AMP + hv + diphosphate
-
-
-
-
?
D-luciferin + dATP + O2
?
-
-
-
-
?
D-luciferin + O2
?
-
10 microM, assay at pH 7.5
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
D-luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
D-luciferin + O2 + ATP
oxyluciferin + AMP + CO2 + light
-
-
-
?
D-luciferin + O2 + ATP
oxyluciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
r
D-luciferyl-adenylate + O2
oxyluciferin + CO2 + AMP + hv
-
-
-
ir
D-luciferyl-AMP + O2
dehydroluciferyl-AMP + H2O2
-
-
-
-
ir
D-luciferyl-O-adenosine monophosphate + ?
?
-
-
-
?
D-naphthylluciferin + ATP + O2
?
-
-
-
?
D-quinolylluciferin + ATP + O2
?
-
-
-
?
decanoic acid + ATP + CoA
decanoyl-CoA + AMP + diphosphate
dehydroluciferin + ATP
dehydroluciferyl-AMP + diphosphate
dehydroluciferin + ATP + O2
diphosphate + dehydroluciferin-adenylate
-
-
-
?
dehydroluciferin + CoA
dehydroluciferyl-CoA
-
-
-
-
?
dehydroluciferyladenylate + CoA
dehydroluciferyl-CoA + ?
-
-
-
-
?
deoxyATP + Photinus luciferin
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
fatty acid + ATP + CoA
fatty acyl-CoA + AMP
-
-
-
?
L-luciferin + CoA + ATP
luciferyl-CoA + AMP + diphosphate
-
L-luciferin is converted to luciferyl adenylate, and the adenyl group of luciferyl adenylate is then substituted to CoA-SH to give luciferyl-CoA. Even in presence of CoA-SH, D-luciferin is used for the light production reaction, but is not converted into luciferyl-CoA
-
-
?
lauric acid + ATP + CoA
lauroyl-CoA + AMP + diphosphate
linoleic acid + ATP + CoA
linoleoyl-CoA + AMP + CoA
linoleic acid + ATP + CoA
linoleoyl-CoA + AMP + diphosphate
-
-
-
-
?
linolenic acid + ATP + CoA
linolenoyl-CoA + AMP + CoA
-
-
-
-
?
linolenic acid + ATP + CoA
linolenoyl-CoA + AMP + diphosphate
-
-
-
-
?
luciferin + ATP + O2
?
-
assay at 25°C, pH 7.8
-
-
?
luciferin + ATP + O2
diadenosine 5',5''-P1,P4-tetraphosphate
-
-
-
r
luciferin + ATP + O2
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
luciferin + ATP + O2
oxyluciferin + AMP + CO2 + diphosphate
-
-
-
-
?
luciferin + MgATP2-
AMP + diphosphate
luciferin + O2 + ATP
?
-
assay at pH 7.8
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
luciferin + O2 + ATP
oxyluciferin + AMP + CO2 + light
-
-
-
-
ir
luciferyl adenylate + O2
dehydroluciferyl adenylate + ?
-
-
-
-
?
luciferyl-adenylate
oxyluciferin + hnu
-
-
-
?
luciferyl-adenylate + O2
oxyluciferin + AMP + CO2 + hv
luciferyl-AMP + O2
oxyluciferin + hv + CO2 + AMP + diphosphate
-
-
-
-
r
luciferyl-O-adenosine monophosphate + ?
?
luciferyl-O-adenosine monophosphate + CoA
luciferyl-CoA + AMP
-
-
-
?
myristic acid + ATP + CoA
myristoyl-CoA + AMP + diphosphate
oleic acid + ATP + CoA
oleoyl-CoA + AMP + diphosphate
P1,P5-di(adenosine-5'-)pentaphosphate + Photinus luciferin
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
2.2% light response relative to ATP
-
?
palmitic acid + ATP + CoA
palmitoyl-CoA + AMP + diphosphate
-
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
Photinus luciferin + O2 + GTP
adenosine 5'-P1-tetraphospho-P4-5'''-guanosine
-
-
-
?
polyethylene glycol-6-amino-D-luciferin
? + hv
-
longer light emission
-
-
?
R-COOH + ATP
R-CO-AMP + diphosphate
additional information
?
-
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ?

5,5-dimethyloxyluciferin + hv
-
-
-
-
?
5,5-dimethyl-luciferyl-O-adenosine monophosphate + ?
5,5-dimethyloxyluciferin + hv
-
-
-
-
?
D-firefly luciferin + O2 + ATP

firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
?
D-firefly luciferin + O2 + ATP
firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
-
-
?
D-luciferin + ATP + O2

?
-
-
-
-
?
D-luciferin + ATP + O2
?
-
assay at pH 7.5, 24-27°C
-
-
?
D-luciferin + ATP + O2

luciferyl-adenylate + diphosphate + H2O + hv
-
-
-
-
?
D-luciferin + ATP + O2
luciferyl-adenylate + diphosphate + H2O + hv
-
-
-
r
D-luciferin + ATP + O2

oxidized D-luciferin + AMP + CO2 + diphosphate
-
-
-
-
?
D-luciferin + ATP + O2
oxidized D-luciferin + AMP + CO2 + diphosphate
-
decay time to 10%: 23 sec, relative specific activity: 100%
-
-
?
D-luciferin + ATP + O2

oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
assay at 25°C, pH 7.8
-
-
?
D-luciferin + ATP + O2
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + ATP + O2

oxidized luciferin + AMP + CO2 + diphosphate + hv
-
-
-
?
D-luciferin + ATP + O2
oxidized luciferin + AMP + CO2 + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP

oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
Macrolampis sp2
-
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
D-luciferin is (S)-2-[6'-hydroxy-2'-benzothiazolyl]-2-thiazoline-4-carboxylic acid (S)-2-[6-hydroxybenzothiazol-2-yl]-4,5-dihydrothiazole-4-carboxylic acid
-
-
r
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
D-luciferin is (S)-2-(6-hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid
the keto form of oxidized D-luciferin produces red light in chemiluminescence and yellow-green in bioluminescence
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP
oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP

oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
D-luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
decanoic acid + ATP + CoA

decanoyl-CoA + AMP + diphosphate
-
-
-
?
decanoic acid + ATP + CoA
decanoyl-CoA + AMP + diphosphate
-
-
-
?
dehydroluciferin + ATP

dehydroluciferyl-AMP + diphosphate
-
-
-
r
dehydroluciferin + ATP
dehydroluciferyl-AMP + diphosphate
-
-
-
r
lauric acid + ATP + CoA

lauroyl-CoA + AMP + diphosphate
-
-
-
?
lauric acid + ATP + CoA
lauroyl-CoA + AMP + diphosphate
-
-
-
?
linoleic acid + ATP + CoA

linoleoyl-CoA + AMP + CoA
-
-
-
-
?
linoleic acid + ATP + CoA
linoleoyl-CoA + AMP + CoA
linoleic acid concentration above 10 microM has an inhibitory effect
-
-
?
luciferin + ATP + O2

oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
luciferin + ATP + O2
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
luciferin + MgATP2-

AMP + diphosphate
-
-
-
?
luciferin + MgATP2-
AMP + diphosphate
-
-
-
-
?
luciferin + MgATP2-
AMP + diphosphate
-
-
-
?
luciferin + O2 + ATP

oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
the enzyme in peroxisomes may keep the catalytic functions in bioluminescence and fatty acid metabolism
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
luciferase modulates emission color by controlling the resonance-based delocalization of the anionic keto form of the oxyluciferin excited state
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
the enzyme in peroxisomes may keep the catalytic functions in bioluminescence and fatty acid metabolism
-
-
?
luciferin + O2 + ATP
oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
luciferase modulates emission color by controlling the resonance-based deloclization of the anionic keto form of the oxyluciferin excited state
-
?
luciferyl-adenylate + O2

oxyluciferin + AMP + CO2 + hv
-
-
-
ir
luciferyl-adenylate + O2
oxyluciferin + AMP + CO2 + hv
-
-
-
ir
luciferyl-O-adenosine monophosphate + ?

?
-
-
-
-
?
luciferyl-O-adenosine monophosphate + ?
?
-
-
-
-
?
myristic acid + ATP + CoA

myristoyl-CoA + AMP + diphosphate
-
-
-
?
myristic acid + ATP + CoA
myristoyl-CoA + AMP + diphosphate
-
-
-
?
oleic acid + ATP + CoA

oleoyl-CoA + AMP + diphosphate
-
-
-
-
?
oleic acid + ATP + CoA
oleoyl-CoA + AMP + diphosphate
-
-
-
-
?
Photinus luciferin + O2 + ATP

oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
highly specific for ATP
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
enzyme produced in Sf9 cells requires less luciferin than mammalian or plant cells
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
the true substrate is MgATP2-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
the true substrate is MgATP2-
-
?
Photinus luciferin + O2 + ATP
oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
the true substrate is MgATP2-
-
?
R-COOH + ATP

R-CO-AMP + diphosphate
-
-
-
r
R-COOH + ATP
R-CO-AMP + diphosphate
-
-
-
r
additional information

?
-
no fatty acyl-CoA synthetase activity with octanoic acid and palmitic acid
-
-
?
additional information
?
-
-
12 nucleoside triphosphates examined
-
-
?
additional information
?
-
no fatty acyl-CoA synthetase activity with octanoic acid and palmitic acid
-
-
?
additional information
?
-
analysis of reaction parameters, detailed overview
-
-
?
additional information
?
-
-
analysis of reaction parameters, detailed overview
-
-
?
additional information
?
-
analysis of reaction parameters, detailed overview
-
-
?
additional information
?
-
-
analysis of reaction parameters, detailed overview
-
-
?
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(2R)-2-[4-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)phenyl]propanoic acid
common name indoprofen
(2Z)-1-(4-chlorophenyl)-3-(pyridin-2-ylamino)prop-2-en-1-one
-
(2Z)-3-[(2-bromophenyl)amino]-1-pyridin-2-ylprop-2-en-1-one
-
(2Z)-3-[(4-fluorophenyl)amino]-1-furan-2-ylprop-2-en-1-one
-
(2Z)-3-[[4-(dimethylamino)cyclohexa-1,5-dien-1-yl]amino]-1-phenylprop-2-en-1-one
-
(3Z)-3-[[4-(dimethylamino)phenyl]methylidene]-1,3-dihydro-2H-indol-2-one
common name SU4312
1-phenyl-3-(5-phenylpyridin-2-yl)urea
-
10 microM, 20% inhibition
1-[3-(6-ethoxy-1,3-benzothiazol-2-yl)thiophen-2-yl]urea
-
2,4-dimethoxy-N-(5-methylpyridin-2-yl)benzamide
2-(2-amino-3-methoxyphenyl)-4H-chromen-4-one
common name PD98059
2-(2-chlorophenyl)-6-methoxy-1,3-benzothiazole
-
2-(2-fluorophenyl)-6-methoxy-1,3-benzothiazole
2-(2-imino-4,5,6,7-tetrahydro-1,3-benzothiazol-3(2H)-yl)-1-(4-methylphenyl)ethanone
common name pifithrin-alpha
2-(2-methoxyphenyl)-1,3-benzothiazole
2-(3,4-dimethoxyphenyl)-1,3-benzothiazole
2-(3,4-dimethoxyphenyl)-6-methoxy-1,3-benzothiazole
2-(3-fluorophenyl)-1,3-benzothiazole
2-(3-fluorophenyl)-6-methoxy-1,3-benzothiazole
2-(4-chlorophenyl)-1,3-benzothiazole
2-(4-chlorophenyl)-6-methoxy-1,3-benzothiazole
2-(4-ethoxyphenyl)-4-[(4-methylpiperazin-1-yl)carbonyl]quinoline
-
2-(4-ethoxyphenyl)-4-[(4-pyridin-2-ylpiperazin-1-yl)carbonyl]quinoline
-
2-(4-fluorophenyl)-6-methoxy-1,3-benzothiazole
2-(4-methoxyphenyl)-1,3-benzothiazole
2-(4-methylphenyl)-4-[(4-pyrimidin-2-ylpiperazin-1-yl)carbonyl]quinoline
-
2-(5-biphenyl-4-yl-1,2,4-oxadiazol-3-yl)pyridine
2-(5-furan-2-yl-1,2,4-oxadiazol-3-yl)pyridine
2-(5-naphthalen-2-yl-1,2,4-oxadiazol-3-yl)pyridine
-
2-(5-pyridin-4-yl-1,2,4-oxadiazol-3-yl)pyridine
2-(6'-hydroxy-2'-benzothiazolyl)-4-hydroxymethylthiazole
-
competitive inhibitor
2-([7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
2-hydroxy-N'-[(1E)-(2-hydroxyphenyl)methylidene]benzohydrazide
common name SCS
2-methyl-6-(phenylethynyl)pyridine
common name MPEB
2-methyl-6-[(Z)-2-phenylethenyl]pyridine
common name SIB1893
2-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]pyridine
2-[5-(3,4-dichlorophenyl)-1,2,4-oxadiazol-3-yl]pyridine
-
2-[5-(3-chloro-4-methylphenyl)-1,2,4-oxadiazol-3-yl]pyridine
-
2-[5-(3-chlorophenyl)-1,2,4-oxadiazol-3-yl]pyridine
-
2-[5-(5-bromofuran-2-yl)-1,2,4-oxadiazol-3-yl]pyridine
-
3,4-dichloro-N-(5-phenylpyridin-2-yl)benzamide
-
10 microM, 13% inhibition
3,4-dimethoxy-N-(5-phenylpyridin-2-yl)benzamide
-
10 microM, 26% inhibition
-
3,5-diphenyl-1,2,4-oxadiazole
-
-
3-(2-hydroxyphenyl)-1H-benzo[f]chromen-1-one
common name flavonoid
3-(2-methoxyphenyl)-5-phenyl-1,2,4-oxadiazole
3-([7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
3-chloro-N-(5-phenylpyridin-2-yl)benzamide
-
-
3-methyl-2-[(E)-phenyldiazenyl]phenol
3-pyridin-2-yl-1,2,4-oxadiazol-5-yl biphenyl-4-carboxylate
3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
decrease of Fluc proteolysis, 2 microM
3-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
3-[5-(3-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
3-[5-(3-methoxyphenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
3-[5-(4-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
3-[5-[2-(trifluoromethyl)phenyl]-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
3-[5-[4-(trifluoromethyl)phenyl]-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
4-((7-[4-(trifluoromethyl)benzyl]-7H-pyrrolo[2,3-d]pyrimidin-4-yl)oxy)aniline
-
7% inhibition
4-(1,3-benzothiazol-2-yl)-N,N-dimethylaniline
4-(1,3-benzothiazol-2-yl)benzonitrile
4-(1,4-dioxa-8-azaspiro[4.5]dec-8-ylcarbonyl)-2-(4-ethoxyphenyl)quinoline
-
4-(1-methylethoxy)-N-(5-phenylpyridin-2-yl)benzamide
-
10 microM, 12% inhibition
-
4-(4-aminophenoxy)-7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-2-amine
-
-
4-(5-furan-2-yl-1,2,4-oxadiazol-3-yl)pyridine
4-(6-methoxy-1,3-benzothiazol-2-yl)-N,N-dimethylaniline
4-(6-methoxy-1,3-benzothiazol-2-yl)benzonitrile
4-(7H-pyrrolo[2,3-d]pyrimidin-4-yloxy)aniline
-
19% inhibition
4-(dimethylamino)-N-(5-phenylpyridin-2-yl)benzamide
-
10 microM, 35% inhibition
4-([2-(methylsulfanyl)-7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
31% inhibition
4-([2-(methylsulfonyl)-7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
9% inhibition
4-([7-(2,2-dimethylpropyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)-N,N-dimethylaniline
-
-
4-([7-(2,2-dimethylpropyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)-N-methylaniline
-
-
4-([7-(2,2-dimethylpropyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
4-([7-(2-methylprop-2-en-1-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
4-([7-(2-methylpropyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
4-([7-(3-methylbut-2-en-1-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
4-([7-(3-methylbutyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
4-([7-(4-methoxybenzyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
26% inhibition
4-([7-(prop-2-en-1-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
4-([7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
4-amino-6-[(E)-[4'-[(Z)-(8-amino-1-hydroxy-5,7-disulfonato-6,7-dihydronaphthalen-2-yl)diazenyl]-3-methylbiphenyl-4-yl]diazenyl]-5-hydroxy-2,3-dihydronaphthalene-1,3-disulfonate
common name Evans Blue
4-amino-N-(5-phenylpyridin-2-yl)benzamide
-
-
4-chloro-N-(5-phenylpyridin-2-yl)benzamide
-
-
4-methoxy-N-(5-phenylpyridin-2-yl)benzamide
-
10 microM, 28% inhibition
4-methoxy-N-[(4-methylpyridin-2-yl)carbamoyl]benzamide
-
4-methyl-N-(5-phenylpyridin-2-yl)benzamide
-
10 microM, 18% inhibition
4-methyl-N-(phenylmethyl)benzenesulfonamide
-
-
4-[(7-benzyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)oxy]aniline
-
46% inhibition
4-[(7-cyclopentyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)oxy]aniline
-
-
4-[(7-ethenyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)oxy]aniline
-
-
4-[(7-ethyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)oxy]aniline
-
-
4-[(7-methyl-7H-pyrrolo[2,3-d]pyrimidin-4-yl)oxy]aniline
-
48% inhibition
4-[1-(1,3-benzothiazol-2-yl)-2-(4-methylpiperazin-1-yl)-2-oxoethyl]phenol
-
4-[3-(2-methoxyphenyl)-1,2,4-oxadiazol-5-yl]pyridine
4-[5-(2-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
4-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
4-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]-N,N-dimethylaniline
4-[5-(3-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
4-[5-(3-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
-
5'-O-[(N-dehydroluciferyl)-sulfamoyl]-adenosine
-
stable and potent reversibel inhibitor
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine
competitive inhibitor
5-(2,4-dimethoxyphenyl)-3-phenyl-1,2,4-oxadiazole
5-(2-bromophenyl)-3-(4-methylphenyl)-1,2,4-oxadiazole
-
5-(2-chloro-4-methylphenyl)-3-phenyl-1,2,4-oxadiazole
-
5-(2-fluorophenyl)-3-(4-methoxyphenyl)-1,2,4-oxadiazole
5-(4-fluorophenyl)-3-(3-methylphenyl)-1,2,4-oxadiazole
5-anilino-1-naphthalene sulfonate
5-methyl-N-[6-(methylsulfonyl)-1,3-benzothiazol-2-yl]thiophene-2-carboxamide
-
5-naphthalen-2-yl-3-phenyl-1,2,4-oxadiazole
-
6-methoxy-2-(2-methoxyphenyl)-1,3-benzothiazole
6-methoxy-2-(3-methylphenyl)-1,3-benzothiazole
6-methoxy-2-(4-methoxyphenyl)-1,3-benzothiazole
6-methoxy-2-[2-(2-methylbenzyl)phenyl]-1,3-benzothiazole
-
6-methoxy-2-[3-(2-methylbenzyl)phenyl]-1,3-benzothiazole
-
6-methyl-2-[(Z)-phenyldiazenyl]pyridin-3-ol
common name SIB1757
6-toluidino-2-naphthalene sulfonate
arachidonic acid
concentration above 10 microM
bovine serum albumin
-
inhibition when present in large excess
-
butanoic acid
-
IC50: 13.6 mM
CoA
-
above 0.1 mM, N-terminal domain
D-luciferin
-
inhibits CoA-ligase activity with L-luciferin, IC50: 0.135 mM against 0.1 mM L-luciferin
Decanoic acid
-
IC50: 0.0132 mM
dehydroluciferyl adenylate
dehydroluciferyl-adenylate
-
tight-binding competitive inhibitor
dehydroluciferyl-CoA
-
IC50: 0.005 mM
Dodecanol
-
mixed-type inhibitor
dodecylamine
-
noncompetitive inhibitor
ethyl 4-[[2-(4-ethoxyphenyl)quinolin-4-yl]carbonyl]piperazine-1-carboxylate
-
ethyl [4-(4-aminophenoxy)-7H-pyrrolo[2,3-d]pyrimidin-7-yl]acetate
-
33% inhibition
ethyl-2-benzothiazole sulfonate
-
competitive inhibitor
geneticin
-
induces nonsense suppression
gentamicin
-
induces nonsense suppression
Halothane
non-competitive inhibitor
hexadecanoic acid
-
IC50: 0.00067 mM
hexanoic acid
-
IC50: 3.4 mM
L-1-tosylamido-2-phenethyl chlorometyl ketone
-
competitive inhibitor with respect to luciferin, noncompetitive with respect to ATP
L-AMP
a potent luciferase inhibitor
Limulus amebocyte lysate
-
decreased luminescence intensity to 10%
-
linoleic acid
concentration above 10 microM
N'-(3-chlorophenyl)-N-[(1Z)-(3-chlorophenyl)methylidene]imidoformamide
common name DCB
N,N-dimethyl-4-([7-(2-methylprop-2-en-1-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
N,N-dimethyl-4-([7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
N-(4-ethoxyphenyl)-2-methoxybenzamide
-
N-(5-methylpyridin-2-yl)benzamide
-
-
N-(5-phenylpyridin-2-yl)benzamide
-
10 microM, 27% inhibition
N-(5-phenylpyridin-2-yl)butanamide
-
10 mciroM, 22% inhibition
N-(5-phenylpyridin-2-yl)cyclohexanecarboxamide
-
-
N-(6-ethoxy-1,3-benzothiazol-2-yl)-2-methylfuran-3-carboxamide
-
N-(6-methoxy-1,3-benzothiazol-2-yl)-2-methylfuran-3-carboxamide
-
N-(6-methoxy-1,3-benzothiazol-2-yl)-3-methylthiophene-2-carboxamide
-
-
N-(6-methyl-1,3-benzothiazol-2-yl)thiophene-2-carboxamide
N-(6-phenylpyridin-2-yl)benzamide
-
10 microM, 30% inhibition
N-benzyl-5-phenylpyridin-2-amine
-
10 microM, 20% inhibition
N-methyl-4-([7-(2-methylprop-2-en-1-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
N-methyl-4-([7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
-
-
N-pyridin-2-ylbenzamide
-
N-quinolin-2-ylbenzamide
-
-
N-tosyl-L-lysine chloromethyl ketone
-
-
N-tosyl-L-phenylalanine chloromethyl ketone
N-[3-(6-methoxy-1,3-benzothiazol-2-yl)phenyl]acetamide
N-[4-(1,3-benzothiazol-2-yl)phenyl]acetamide
N-[5-(3,4-dichlorophenyl)pyridin-2-yl]benzamide
-
10 microM, 4% inhibition
N-[5-(3-chlorophenyl)pyridin-2-yl]benzamide
-
10 microM, 38% inhibition
N-[5-(4-chlorophenyl)pyridin-2-yl]benzamide
-
-
N-[5-(4-methoxyphenyl)pyridin-2-yl]benzamide
-
-
N-[5-(4-methylphenyl)pyridin-2-yl]benzamide
-
10 microM, 51% inhibition
N-[5-(4-tert-butylphenyl)pyridin-2-yl]benzamide
-
10 microM, 49% inhibition
N-[5-[(2,2-dimethylpropanoyl)amino]pyridin-2-yl]-3-hydroxybenzamide
-
N-[5-[4-(1-methylethoxy)phenyl]pyridin-2-yl]benzamide
-
10 microM, 38% inhibition
N-[5-[4-(dimethylamino)phenyl]pyridin-2-yl]benzamide
-
10 microM, 14% inhibition
N-[5-[4-(trifluoromethyl)phenyl]pyridin-2-yl]benzamide
-
10 microM, 39% inhibition
N-[6-(methylsulfonyl)-1,3-benzothiazol-2-yl]thiophene-2-carboxamide
-
octadecanoic acid
-
IC50: 0.00063 mM
octanoic acid
-
IC50: 2.9 mM
oleic acid
concentration above 10 microM
phenyl (5-phenylpyridin-2-yl)carbamate
-
-
-
pifithrin-alpha
20 microM, above 95% inhibition
resveratrol
-
resveratrol is 3,5,4'-trihydroxystilbene, potent inhibitor
tetradecanoic acid
-
IC50: 0.00068 mM
2,4-dimethoxy-N-(5-methylpyridin-2-yl)benzamide

-
-
2,4-dimethoxy-N-(5-methylpyridin-2-yl)benzamide
-
2-(2-chlorophenyl)-6-methoxy-1,3-benzothiazole

-
-
-
2-(2-chlorophenyl)-6-methoxy-1,3-benzothiazole
-
-
-
2-(2-fluorophenyl)-6-methoxy-1,3-benzothiazole

-
-
2-(2-fluorophenyl)-6-methoxy-1,3-benzothiazole
-
-
2-(2-methoxyphenyl)-1,3-benzothiazole

-
-
2-(2-methoxyphenyl)-1,3-benzothiazole
-
-
2-(3,4-dimethoxyphenyl)-1,3-benzothiazole

-
-
2-(3,4-dimethoxyphenyl)-1,3-benzothiazole
-
-
2-(3,4-dimethoxyphenyl)-6-methoxy-1,3-benzothiazole

-
-
2-(3,4-dimethoxyphenyl)-6-methoxy-1,3-benzothiazole
-
-
2-(3-fluorophenyl)-1,3-benzothiazole

-
-
2-(3-fluorophenyl)-1,3-benzothiazole
-
-
2-(3-fluorophenyl)-6-methoxy-1,3-benzothiazole

-
-
2-(3-fluorophenyl)-6-methoxy-1,3-benzothiazole
-
-
2-(4-chlorophenyl)-1,3-benzothiazole

-
-
2-(4-chlorophenyl)-1,3-benzothiazole
-
-
2-(4-chlorophenyl)-6-methoxy-1,3-benzothiazole

-
-
2-(4-chlorophenyl)-6-methoxy-1,3-benzothiazole
-
-
2-(4-fluorophenyl)-6-methoxy-1,3-benzothiazole

-
-
2-(4-fluorophenyl)-6-methoxy-1,3-benzothiazole
-
-
2-(4-methoxyphenyl)-1,3-benzothiazole

-
-
2-(4-methoxyphenyl)-1,3-benzothiazole
-
-
2-(5-biphenyl-4-yl-1,2,4-oxadiazol-3-yl)pyridine

-
2-(5-biphenyl-4-yl-1,2,4-oxadiazol-3-yl)pyridine
-
-
2-(5-biphenyl-4-yl-1,2,4-oxadiazol-3-yl)pyridine
-
-
2-(5-furan-2-yl-1,2,4-oxadiazol-3-yl)pyridine

-
-
2-(5-furan-2-yl-1,2,4-oxadiazol-3-yl)pyridine
-
-
2-(5-pyridin-4-yl-1,2,4-oxadiazol-3-yl)pyridine

-
-
2-(5-pyridin-4-yl-1,2,4-oxadiazol-3-yl)pyridine
-
-
2-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]pyridine

-
-
2-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]pyridine
-
-
3',5'-cyclic AMP

-
competitive inhibitor
3-(2-methoxyphenyl)-5-phenyl-1,2,4-oxadiazole

-
-
3-(2-methoxyphenyl)-5-phenyl-1,2,4-oxadiazole
-
-
3-methyl-2-[(E)-phenyldiazenyl]phenol

-
-
3-methyl-2-[(E)-phenyldiazenyl]phenol
-
-
3-pyridin-2-yl-1,2,4-oxadiazol-5-yl biphenyl-4-carboxylate

-
-
3-pyridin-2-yl-1,2,4-oxadiazol-5-yl biphenyl-4-carboxylate
-
-
4-(1,3-benzothiazol-2-yl)-N,N-dimethylaniline

-
-
4-(1,3-benzothiazol-2-yl)-N,N-dimethylaniline
-
-
4-(1,3-benzothiazol-2-yl)benzonitrile

-
-
4-(1,3-benzothiazol-2-yl)benzonitrile
-
-
4-(5-furan-2-yl-1,2,4-oxadiazol-3-yl)pyridine

-
-
4-(5-furan-2-yl-1,2,4-oxadiazol-3-yl)pyridine
-
-
4-(6-methoxy-1,3-benzothiazol-2-yl)-N,N-dimethylaniline

-
-
4-(6-methoxy-1,3-benzothiazol-2-yl)-N,N-dimethylaniline
-
-
4-(6-methoxy-1,3-benzothiazol-2-yl)benzonitrile

-
-
4-(6-methoxy-1,3-benzothiazol-2-yl)benzonitrile
-
-
4-[3-(2-methoxyphenyl)-1,2,4-oxadiazol-5-yl]pyridine

-
-
4-[3-(2-methoxyphenyl)-1,2,4-oxadiazol-5-yl]pyridine
-
-
4-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]-N,N-dimethylaniline

-
-
4-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]-N,N-dimethylaniline
-
-
5-(2,4-dimethoxyphenyl)-3-phenyl-1,2,4-oxadiazole

-
5-(2,4-dimethoxyphenyl)-3-phenyl-1,2,4-oxadiazole
-
-
5-(2,4-dimethoxyphenyl)-3-phenyl-1,2,4-oxadiazole
-
-
5-(2-fluorophenyl)-3-(4-methoxyphenyl)-1,2,4-oxadiazole

-
-
5-(2-fluorophenyl)-3-(4-methoxyphenyl)-1,2,4-oxadiazole
-
-
5-(4-fluorophenyl)-3-(3-methylphenyl)-1,2,4-oxadiazole

-
-
5-(4-fluorophenyl)-3-(3-methylphenyl)-1,2,4-oxadiazole
-
-
5-anilino-1-naphthalene sulfonate

-
-
5-anilino-1-naphthalene sulfonate
-
competitive inhibitor
6-methoxy-2-(2-methoxyphenyl)-1,3-benzothiazole

-
-
6-methoxy-2-(2-methoxyphenyl)-1,3-benzothiazole
-
-
6-methoxy-2-(3-methylphenyl)-1,3-benzothiazole

-
-
6-methoxy-2-(3-methylphenyl)-1,3-benzothiazole
-
-
6-methoxy-2-(4-methoxyphenyl)-1,3-benzothiazole

-
-
6-methoxy-2-(4-methoxyphenyl)-1,3-benzothiazole
-
-
6-methoxy-2-[2-(2-methylbenzyl)phenyl]-1,3-benzothiazole

-
-
-
6-methoxy-2-[2-(2-methylbenzyl)phenyl]-1,3-benzothiazole
-
-
-
6-methoxy-2-[3-(2-methylbenzyl)phenyl]-1,3-benzothiazole

-
-
-
6-methoxy-2-[3-(2-methylbenzyl)phenyl]-1,3-benzothiazole
-
-
-
6-toluidino-2-naphthalene sulfonate

-
-
6-toluidino-2-naphthalene sulfonate
-
competitive inhibitor
arsenate

-
lowers the flash height and extends the light emission for a given amount of ATP
arsenate
-
reversible inhibition
arsenate
-
lowers the flash height and extends the light emission for a given amount of ATP
ATP

-
-
ATP
-
uncomplexed ATP is a competitive inhibitor with respect to MgATP2- complex
ATP
-
noncompetitive inhibitor
ATP
-
uncomplexed ATP is a competitive inhibitor with respect to MgATP2- complex
dehydroluciferin

-
dehydroluciferin
-
competitive inhibitor
dehydroluciferyl adenylate

-
dehydroluciferyl adenylate
-
IC50: 6 nM
dehydroluciferyl adenylate
-
diphosphate

-
-
diphosphate
depending on conditions
dodecanoic acid

-
IC50: 0.0012 mM
dodecanoic acid
-
competitive
L-luciferin

-
Luciferin

-
-
Luciferin
-
noncompetitive inhibitor
Luciferin
-
inhibition if concentration is higher than 0.05 mM
N-(6-methyl-1,3-benzothiazol-2-yl)thiophene-2-carboxamide

-
N-(6-methyl-1,3-benzothiazol-2-yl)thiophene-2-carboxamide
-
-
N-tosyl-L-phenylalanine chloromethyl ketone

-
-
N-tosyl-L-phenylalanine chloromethyl ketone
-
competitive inhibitor with respect to luciferin, noncompetitive with respect to ATP, pH dependent, enzyme protected by dehydroluciferin-AMP
N-[3-(6-methoxy-1,3-benzothiazol-2-yl)phenyl]acetamide

-
-
N-[3-(6-methoxy-1,3-benzothiazol-2-yl)phenyl]acetamide
-
-
N-[4-(1,3-benzothiazol-2-yl)phenyl]acetamide

-
-
N-[4-(1,3-benzothiazol-2-yl)phenyl]acetamide
-
-
oxyluciferin

-
oxyluciferin
-
competitive inhibitor
Procion blue MX-R

-
irreversible inhibitor, enzyme protected by luciferin, ATP and MgATP2-
Procion blue MX-R
-
irreversible inhibitor, enzyme protected by luciferin, ATP and MgATP2-; time dependent
additional information

-
binding of one of the substrates ATP or luciferin to luciferase decreases the affinity of the enzyme to the other substrate
-
additional information
irreversible aggregation because of the exposure of its hydrophobic sites followed by structural changes leads to its further inactivation
-
additional information
-
irreversible aggregation because of the exposure of its hydrophobic sites followed by structural changes leads to its further inactivation
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.176
(4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 8.5, temperature not specified in the publication
0.07
(4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 8.5, temperature not specified in the publication
0.061
(4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 9.1, temperature not specified in the publication
0.02
(4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid
-
pH 8.5, temperature not specified in the publication
0.00025 - 0.0008
5,5-dimethyl-luciferyl-O-adenosine monophosphate
0.014 - 0.024
D-firefly luciferin
0.00038 - 0.0126
D-luciferyl-O-adenosine monophosphate
0.0149
dehydroluciferyl-adenylate
-
-
0.00741 - 0.0163
lauric acid
0.0136
linolenic acid
-
-
0.0006 - 0.0127
luciferyl-O-adenosine monophosphate
additional information
additional information
-
0.00025
5,5-dimethyl-luciferyl-O-adenosine monophosphate

-
pH 8.6, wild-type enzyme
0.0003
5,5-dimethyl-luciferyl-O-adenosine monophosphate
-
pH 8.6, mutant enzyme F250S
0.0003
5,5-dimethyl-luciferyl-O-adenosine monophosphate
-
pH 8.6, mutant enzyme G246A
0.0003
5,5-dimethyl-luciferyl-O-adenosine monophosphate
-
pH 8.6, wild-type enzyme
0.0004
5,5-dimethyl-luciferyl-O-adenosine monophosphate
-
pH 8.6, mutant enzyme A243G
0.0008
5,5-dimethyl-luciferyl-O-adenosine monophosphate
-
pH 8.6, mutant enzyme S247F
0.00015
ATP

-
-
0.00015
ATP
-
immobilized on activated Sepharose
0.001
ATP
mutant enzyme I423L/D436G/L530R, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.002
ATP
-
immobilized enzyme
0.003
ATP
-
150 microM luciferin, two-site binding model
0.0038
ATP
-
150 microM luciferin, presence of 1 microM of N-quinolin-2-ylbenzamide, two-site binding model
0.004
ATP
free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol
0.008
ATP
mutant I423L/D436G/L530R, in the presence of 0.001 mM D-luciferin
0.009
ATP
mutant enzyme D436G, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.009
ATP
mutant enzyme D436G, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.009
ATP
mutant D436G, in the presence of 0.001 mM D-luciferin
0.029
ATP
recombinant mutant enzyme G246A, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.04
ATP
mutant E354K/ins356K
0.041
ATP
recombinant mutant enzyme V241I/G246A/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.0456
ATP
mutant enzyme I432L, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.047
ATP
mutant enzyme I423L, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.047
ATP
mutant I423L, in the presence of 0.001 mM D-luciferin
0.0485
ATP
-
addition of luciferin 30 min after ATP
0.05
ATP
mutant E354Q/ins356K
0.0538
ATP
mutant enzyme L530R, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.054
ATP
mutant enzyme L530R, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.054
ATP
mutant L530R, in the presence of 0.001 mM D-luciferin
0.0594
ATP
-
addition of luciferin 30 min before ATP
0.06
ATP
-
adenylate kinase activity
0.06
ATP
recombinant mutant enzyme G246A/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.06
ATP
recombinant mutant enzyme G246A/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.06
ATP
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][acetate]
0.061
ATP
recombinant mutant enzyme G246A/F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.071
ATP
wild-type luciferase
0.077
ATP
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][acetate]
0.0778
ATP
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][lactate]
0.0778
ATP
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][propionate]
0.08
ATP
pH 7.8, 22°C, recombinant mutant Y255F
0.086
ATP
-
0.094 mM luciferin, in presence of alpha-synuclein
0.086
ATP
pH 7.8, 22°C, recombinant wild-type enzyme
0.093
ATP
recombinant mutant enzyme V241I/G246A/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.094
ATP
-
150 microM luciferin, presence of 1 microM of N-quinolin-2-ylbenzamide, two-site binding model
0.095
ATP
mutant E354R/ins356K
0.0955
ATP
in 50 mM Tricine-NaOH buffer with 10 mM MgSO4, at pH 7.8
0.1
ATP
-
35°C, pH 8.5, mutant A296C/A326C
0.1
ATP
-
mutant D476N, pH 7, 25°C
0.1
ATP
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][triflouroacetate]
0.1
ATP
-
wild-type, pH 8, 25°C
0.1 - 1
ATP
-
pH 7.8, temperature not specified in the publication, native enzyme
0.11
ATP
-
soluble and immobilized enzyme
0.11
ATP
-
two catalytically active sites: first site with a Km for ATP of 0.11 mM is responsible for initial flash, a second site with a Km for ATP of 0.02 mM catalyses the continuous low production of light
0.111
ATP
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][trichloroacetate]
0.111
ATP
-
pH 7.8, temperature not specified in the publication, native enzyme
0.1125
ATP
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][lactate]
0.114
ATP
-
0.094 mM luciferin, without alpha-synuclein
0.114
ATP
-
0.188 mM luciferin, in presence of alpha-synuclein
0.12
ATP
wild-type luciferase
0.125
ATP
-
tricine buffer
0.125
ATP
-
25°C, pH 8, wild-type
0.126
ATP
pH 7.8, 22°C, recombinant mutant R213K/T214N
0.133
ATP
recombinant mutant enzyme F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.133
ATP
-
wild-type luciferase
0.135
ATP
wild-type luciferase
0.135
ATP
pH 7.8, 22°C, recombinant wild-type enzyme
0.138
ATP
-
150 microM luciferin, two-site binding model
0.14
ATP
at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4
0.15
ATP
-
mutant D474K, pH 8.5, 25°C
0.151
ATP
recombinant mutant enzyme V241I/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.157
ATP
-
0.188 mM luciferin, without alpha-synuclein
0.159
ATP
pH 7.8, 22°C, recombinant mutant F255Y
0.16
ATP
recombinant mutant enzyme V241I/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.16
ATP
recombinant wild type enzyme, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.16
ATP
wild type enzyme, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.16
ATP
wild-type, in the presence of 0.001 mM D-luciferin
0.1602
ATP
wild type enzyme, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.166
ATP
recombinant mutant enzyme F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.182
ATP
recombinant mutant enzyme V241I/F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.2
ATP
-
phosphate buffer
0.2
ATP
-
25°C, pH 8.5, mutant I232R
0.2
ATP
-
35°C, pH 8, mutant A296C/A326C/I232R
0.215
ATP
recombinant mutant enzyme F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.23
ATP
-
wild-type enzyme
0.283
ATP
recombinant mutant enzyme V241I, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.285
ATP
-
glycylglycine buffer
0.7
ATP
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][propionate]
1.666
ATP
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][triflouroacetate]
2.25
ATP
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][trichloroacetate]
6.9
ATP
-
N-terminal domain
0.014
D-firefly luciferin

pH 7.8, 22°C, recombinant mutant Y255F
0.015
D-firefly luciferin
pH 7.8, 22°C, recombinant mutant F255Y
0.015
D-firefly luciferin
pH 7.8, 22°C, recombinant wild-type enzyme
0.019
D-firefly luciferin
pH 7.8, 22°C, recombinant wild-type enzyme
0.024
D-firefly luciferin
pH 7.8, 22°C, recombinant mutant R213K/T214N
0.001
D-luciferin

wild type enzyme, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.001
D-luciferin
mutant I423L/D436G/L530R, in the presence of 0.01 mM ATP
0.0018
D-luciferin
mutant enzyme D436G, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.002
D-luciferin
mutant enzyme D436G, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.002
D-luciferin
mutant D436G, in the presence of 0.01 mM ATP
0.0023
D-luciferin
mutant enzyme K443A
0.0024
D-luciferin
native luciferase
0.0024
D-luciferin
native luciferase
0.0037
D-luciferin
mutant enzyme L530R, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.004
D-luciferin
mutant enzyme I423L, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.004
D-luciferin
mutant enzyme L530R, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.004
D-luciferin
recombinant mutant enzyme G246A, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.004
D-luciferin
mutant I423L, in the presence of 0.01 mM ATP
0.004
D-luciferin
mutant L530R, in the presence of 0.01 mM ATP
0.0042
D-luciferin
-
mutant enzyme G246A
0.0043
D-luciferin
mutant enzyme I432L, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.0047
D-luciferin
recombinant luciferase
0.0047
D-luciferin
recombinant luciferase
0.006
D-luciferin
mutant enzyme G446I
0.006
D-luciferin
mutant enzyme Q448A
0.006
D-luciferin
recombinant mutant enzyme V241I/G246A/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.006
D-luciferin
mutant ins356E
0.008
D-luciferin
recombinant mutant enzyme V241I, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.008
D-luciferin
-
mutant D476N, pH 7, 25°C
0.0089
D-luciferin
mutant enzyme K445Q
0.009
D-luciferin
recombinant mutant enzyme V241I/G246A/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.009
D-luciferin
mutant ins356K
0.01
D-luciferin
recombinant mutant enzyme G246A/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.01
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][acetate]
0.01
D-luciferin
-
pH 7.8, temperature not specified in the publication, native enzyme
0.011
D-luciferin
recombinant mutant enzyme G246A/F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.011
D-luciferin
recombinant mutant enzyme G246A/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.011
D-luciferin
mutant E354K/ins356K
0.011
D-luciferin
wild-type luciferase
0.012
D-luciferin
recombinant mutant enzyme V241I/F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.012
D-luciferin
mutant E354Q/ins356K
0.0125
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][lactate]
0.013
D-luciferin
mutant E354K
0.014
D-luciferin
recombinant mutant enzyme F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.014
D-luciferin
mutant E354R/ins356K
0.015
D-luciferin
-
wild-type enzyme
0.015
D-luciferin
wild-type enzyme
0.015
D-luciferin
-
mutant enzyme H245A
0.015
D-luciferin
recombinant wild type enzyme, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.015
D-luciferin
mutant ins356R
0.015
D-luciferin
-
wild-type, pH 8, 25°C
0.0157
D-luciferin
-
mutant enzyme F250G
0.016
D-luciferin
recombinant mutant enzyme F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.016
D-luciferin
mutant E354R
0.016
D-luciferin
-
mutant D474K, pH 8.5, 25°C
0.01612
D-luciferin
wild-type luciferase
0.018
D-luciferin
-
mutant enzyme F247Y
0.018
D-luciferin
-
mutant enzyme G316A
0.018
D-luciferin
mutant ins356Q
0.02
D-luciferin
at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4
0.02
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][triflouroacetate]
0.021
D-luciferin
mutant enzyme I423L/D436G/L530R, in 50 mM Tris/HCl, at pH 7.4 and in the presence of 10 mM MgCl2
0.021
D-luciferin
wild-type, in the presence of 0.001 mM D-luciferin
0.0211
D-luciferin
wild type enzyme, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2
0.022
D-luciferin
-
mutant enzyme F250S
0.022
D-luciferin
recombinant mutant enzyme F250S, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.023
D-luciferin
mutant H245N
0.023
D-luciferin
wild-type luciferase
0.024
D-luciferin
recombinant mutant enzyme V241I/F250G, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.024
D-luciferin
recombinant mutant enzyme V241I/F250T, in 25 mM glycylglycine buffer, at pH 7.8, in the presence of 2 mM Mg2+, at 25°C
0.026
D-luciferin
mutant H431Y
0.028
D-luciferin
-
pH 8.2, temperature not specified in the publication
0.03
D-luciferin
at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4
0.03
D-luciferin
mutant S284T
0.06
D-luciferin
-
mutant enzyme G341A
0.0625
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][lactate]
0.067
D-luciferin
mutant enzyme K443A/K529A
0.07
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][trichloroacetate]
0.085
D-luciferin
-
mutant enzyme I351A
0.099
D-luciferin
-
mutant enzyme T343A
0.1
D-luciferin
-
35°C, pH 8.5, mutant A296C/A326C
0.124
D-luciferin
-
mutant enzyme F247L
0.125
D-luciferin
-
25°C, pH 8, wild-type
0.13
D-luciferin
-
mutant enzyme L342A
0.133
D-luciferin
-
mutant enzyme A348V
0.17
D-luciferin
-
mutant enzyme S347A
0.2
D-luciferin
-
mutant enzyme G315A
0.2
D-luciferin
-
25°C, pH 8.5, mutant I232R
0.2
D-luciferin
-
35°C, pH 8, mutant A296C/A326C/I232R
0.2
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][propionate]
0.23
D-luciferin
-
mutant enzyme F247A
0.23
D-luciferin
-
mutant enzyme K529A
0.23
D-luciferin
mutant enzyme K529A
0.301
D-luciferin
-
mutant enzyme R218A
0.311
D-luciferin
-
mutant enzyme T251A
0.5
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][triflouroacetate]
1
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][acetate]
1
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][propionate]
2.5
D-luciferin
-
pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][trichloroacetate]
0.00038
D-luciferyl-O-adenosine monophosphate

mutant enzyme K443A
0.00055
D-luciferyl-O-adenosine monophosphate
mutant enzyme K443A/K529A
0.0015
D-luciferyl-O-adenosine monophosphate
mutant enzyme G446I
0.0022
D-luciferyl-O-adenosine monophosphate
mutant enzyme Q448A
0.0039
D-luciferyl-O-adenosine monophosphate
mutant enzyme K445Q
0.0047
D-luciferyl-O-adenosine monophosphate
wild-type enzyme
0.0126
D-luciferyl-O-adenosine monophosphate
mutant enzyme K529A
0.00741
lauric acid

-
0.0072
Luciferin

-
wild-type enzyme
0.0079
Luciferin
-
addition of luciferin 30 min before ATP
0.008
Luciferin
free enzyme, in 0.02 M Tris-acetate buffer, pH 7.8, containing 12 mM magnesium acetate, 0.2 mM EDTA, and 0.3 mM dithiothreitol
0.0083
Luciferin
-
addition of luciferin 30 min after ATP
0.01
Luciferin
-
mutant R213M
0.012
Luciferin
-
immobilized on activated Sepharose
0.012
Luciferin
-
90 microM ATP
0.012
Luciferin
-
wild-type luciferase
0.013
Luciferin
-
mutant Q338P
0.014
Luciferin
-
mutant R213E
0.016
Luciferin
-
90 microM ATP, presence of 0.1 microM of N-quinolin-2-ylbenzamide
0.018
Luciferin
-
mutant R337Q
0.026
Luciferin
-
N-terminal domain
0.042
Luciferin
-
phosphate buffer
0.053
Luciferin
-
90 microM ATP, presence of 1 microM of N-quinolin-2-ylbenzamide
0.055
Luciferin
-
glycylglycine buffer
0.074
Luciferin
-
0.5 mM ATP, in presence of alpha-synuclein
0.088
Luciferin
-
0.2 mM ATP, in presence of alpha-synuclein
0.134
Luciferin
-
0.15 mM ATP, in presence of alpha-synuclein
0.188
Luciferin
-
0.15 mM ATP, without alpha-synuclein
0.189
Luciferin
-
0.5 mM ATP, without alpha-synuclein
0.192
Luciferin
-
0.2 mM ATP, without alpha-synuclein
0.197
Luciferin
-
0.1 mM ATP, without alpha-synuclein
0.218
Luciferin
-
0.1 mM ATP, in presence of alpha-synuclein
0.0006
luciferyl-O-adenosine monophosphate

-
pH 8.6, mutant enzyme A243G
0.0008
luciferyl-O-adenosine monophosphate
-
pH 8.6, wild-type enzyme
0.0046
luciferyl-O-adenosine monophosphate
-
pH 8.6, mutant enzyme S247F
0.0077
luciferyl-O-adenosine monophosphate
-
pH 8.6, wild-type enzyme
0.0114
luciferyl-O-adenosine monophosphate
-
pH 8.6, mutant enzyme F250S
0.0127
luciferyl-O-adenosine monophosphate
-
pH 8.6, mutant enzyme G246A
0.025
MgATP2-

-
-
0.029
MgATP2-
-
mutant enzyme G246A
0.0486
MgATP2-
mutant enzyme K443A
0.069
MgATP2-
mutant enzyme K445Q
0.073
MgATP2-
mutant enzyme G446I
0.08
MgATP2-
-
mutant enzyme F247Y
0.107
MgATP2-
-
mutant enzyme G316A
0.133
MgATP2-
-
mutant enzyme F250S
0.16
MgATP2-
-
wild-type enzyme
0.16
MgATP2-
wild-type enzyme
0.166
MgATP2-
-
mutant enzyme F250G
0.2
MgATP2-
-
mutant enzyme G341A
0.226
MgATP2-
-
mutant enzyme F247L
0.24
MgATP2-
-
mutant enzyme H245A
0.285
MgATP2-
-
mutant enzyme I351A
0.341
MgATP2-
mutant enzyme Q448A
0.344
MgATP2-
-
mutant enzyme S347A
0.56
MgATP2-
mutant enzyme K443A/K529A
0.857
MgATP2-
-
mutant enzyme T343A
1.038
MgATP2-
-
mutant enzyme A348V
1.2
MgATP2-
-
mutant enzyme G315A
1.2
MgATP2-
-
mutant enzyme K529A
1.2
MgATP2-
mutant enzyme K529A
1.2
MgATP2-
-
mutant enzyme L342A
2
MgATP2-
-
mutant enzyme T251A
3.5
MgATP2-
-
mutant enzyme F247A
6.2
MgATP2-
-
mutant enzyme R218A
additional information
additional information

-
increase of the Km with increasing ionic strength
-
additional information
additional information
the Km value for the reaction intermediate D-firefly luciferin-AMP is 0.14 mM
-
additional information
additional information
-
the Km value for the reaction intermediate D-firefly luciferin-AMP is 0.14 mM
-
additional information
additional information
the Km value for the reaction intermediate D-firefly luciferin-AMP is 0.24 mM
-
additional information
additional information
-
the Km value for the reaction intermediate D-firefly luciferin-AMP is 0.24 mM
-
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0.0002
(2R)-2-[4-(1-oxo-1,3-dihydro-2H-isoindol-2-yl)phenyl]propanoic acid
Photinus pyralis
-
0.002
(2Z)-1-(4-chlorophenyl)-3-(pyridin-2-ylamino)prop-2-en-1-one
Photinus pyralis
-
0.00008
(2Z)-3-[(2-bromophenyl)amino]-1-pyridin-2-ylprop-2-en-1-one
Photinus pyralis
-
0.001
(2Z)-3-[(4-fluorophenyl)amino]-1-furan-2-ylprop-2-en-1-one
Photinus pyralis
-
0.00021
(2Z)-3-[[4-(dimethylamino)cyclohexa-1,5-dien-1-yl]amino]-1-phenylprop-2-en-1-one
Photinus pyralis
-
0.0008
(3Z)-3-[[4-(dimethylamino)phenyl]methylidene]-1,3-dihydro-2H-indol-2-one
Photinus pyralis
-
0.0006
1-[3-(6-ethoxy-1,3-benzothiazol-2-yl)thiophen-2-yl]urea
Photinus pyralis
-
0.00008
2,4-dimethoxy-N-(5-methylpyridin-2-yl)benzamide
Photinus pyralis
-
0.0062
2-(2-amino-3-methoxyphenyl)-4H-chromen-4-one
Photinus pyralis
-
0.0049 - 0.0054
2-(2-chlorophenyl)-6-methoxy-1,3-benzothiazole
-
0.0005 - 0.0006
2-(2-fluorophenyl)-6-methoxy-1,3-benzothiazole
0.00032
2-(2-imino-4,5,6,7-tetrahydro-1,3-benzothiazol-3(2H)-yl)-1-(4-methylphenyl)ethanone
Photinus pyralis
-
0.0077 - 0.0113
2-(2-methoxyphenyl)-1,3-benzothiazole
0.0019 - 0.0032
2-(3,4-dimethoxyphenyl)-1,3-benzothiazole
0.0028 - 0.0038
2-(3,4-dimethoxyphenyl)-6-methoxy-1,3-benzothiazole
0.0078 - 0.0242
2-(3-fluorophenyl)-1,3-benzothiazole
0.0011 - 0.0015
2-(3-fluorophenyl)-6-methoxy-1,3-benzothiazole
0.0014 - 0.0045
2-(4-chlorophenyl)-1,3-benzothiazole
0.0035 - 0.0038
2-(4-chlorophenyl)-6-methoxy-1,3-benzothiazole
0.0013
2-(4-ethoxyphenyl)-4-[(4-methylpiperazin-1-yl)carbonyl]quinoline
Photinus pyralis
-
0.00041
2-(4-ethoxyphenyl)-4-[(4-pyridin-2-ylpiperazin-1-yl)carbonyl]quinoline
Photinus pyralis
-
0.00086 - 0.0013
2-(4-fluorophenyl)-6-methoxy-1,3-benzothiazole
0.00036 - 0.001
2-(4-methoxyphenyl)-1,3-benzothiazole
0.004
2-(4-methylphenyl)-4-[(4-pyrimidin-2-ylpiperazin-1-yl)carbonyl]quinoline
Photinus pyralis
-
0.000038 - 0.0001
2-(5-biphenyl-4-yl-1,2,4-oxadiazol-3-yl)pyridine
0.0013 - 0.0171
2-(5-furan-2-yl-1,2,4-oxadiazol-3-yl)pyridine
0.0001
2-(5-naphthalen-2-yl-1,2,4-oxadiazol-3-yl)pyridine
Photinus pyralis
-
0.0019 - 0.0144
2-(5-pyridin-4-yl-1,2,4-oxadiazol-3-yl)pyridine
0.0073
2-([7-(propan-2-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy)aniline
Photinus pyralis
-
pH 7.8, 20°C
0.0008
2-hydroxy-N'-[(1E)-(2-hydroxyphenyl)methylidene]benzohydrazide
Photinus pyralis
-
0.0047
2-methyl-6-(phenylethynyl)pyridine
Photinus pyralis
-
0.0043
2-methyl-6-[(Z)-2-phenylethenyl]pyridine
Photinus pyralis
-
0.00008 - 0.0028
2-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]pyridine
0.00084
2-[5-(3,4-dichlorophenyl)-1,2,4-oxadiazol-3-yl]pyridine
Photinus pyralis
-
0.00022
2-[5-(3-chloro-4-methylphenyl)-1,2,4-oxadiazol-3-yl]pyridine
Photinus pyralis
-
0.0005
2-[5-(3-chlorophenyl)-1,2,4-oxadiazol-3-yl]pyridine
Photinus pyralis
-
0.0032
2-[5-(5-bromofuran-2-yl)-1,2,4-oxadiazol-3-yl]pyridine
Photinus pyralis
-
0.00005
3-(2-hydroxyphenyl)-1H-benzo[f]chromen-1-one
Photinus pyralis
-
0.0006 - 0.0083
3-(2-methoxyphenyl)-5-phenyl-1,2,4-o