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Literature summary for 1.13.12.7 extracted from

  • Salehi-Sedeh, H.; Ataei, F.; Jarchi, S.; Hamidi, R.; Hosseinkhani, S.
    Effect of mutation at positively charged residues (K329 and R330) in a flexible region of firefly luciferase on structure and kinetic properties (2019), Enzyme Microb. Technol., 131, 109424 .
    View publication on PubMed
Search for more literature for 1.13.12.7

Protein Variants

Protein Variants Comment Organism
K329I point mutation does not affect the orientation of critical residues in bioluminescence color determination. Thermostability and Km value for luciferin are decreased as compared to wild type enzyme. Intrinsic fluorescence and far-UV CD intensity in K329I mutant is decreased. Realative activity as compared to wild-type enzyme is 32% Photinus pyralis
R330Q point mutation does not affect the orientation of critical residues in bioluminescence color determination. Thermostability and Km value for luciferin are decreased as compared to wild type enzyme. Increase in tryptophan fluorescence intensity and secondary structure content for R330Q as compared with wild type. Realative activity as compared to wild-type enzyme is 23% Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 D-firefly luciferin pH 7.8, 25°C, wild-type enzyme Photinus pyralis
0.0225
-
 D-firefly luciferin pH 7.8, 25°C, mutant enzyme K329I Photinus pyralis
0.05
-
 ATP pH 7.8, 25°C, wild-type enzyme Photinus pyralis
0.05
-
 ATP pH 7.8, 25°C, mutant enzyme R330Q Photinus pyralis
0.075
-
 ATP pH 7.8, 25°C, mutant enzyme K329I Photinus pyralis
0.1421
-
 D-firefly luciferin pH 7.8, 25°C, mutant enzyme R330Q Photinus pyralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-firefly luciferin + O2 + ATP Photinus pyralis
-
 firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?

Organism

Organism UniProt Comment Textmining
Photinus pyralis P08659
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-firefly luciferin + O2 + ATP
-
 Photinus pyralis firefly oxyluciferin + CO2 + AMP + diphosphate + hv
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 wild-type enzyme and mutant enzymes R330Q and K329I Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 pH 7.8, 25°C, mutant enzyme K329I Photinus pyralis
7.5 8 pH 7.8, 25°C, wild-type enzyme Photinus pyralis
8
-
 pH 7.8, 25°C, mutant enzyme R330Q Photinus pyralis