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Literature summary for 1.13.12.7 extracted from

  • Noda, K.; Goto, H.; Murakami, Y.; Ahmed, A.B.; Kuroda, A.
    Endotoxin assay by bioluminescence Using mutant firefly luciferase (2010), Anal. Biochem., 397, 152-155.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
MS-275
-
Photinus pyralis

Application

Application Comment Organism
medicine use in endotoxin detection method Photinus pyralis

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cells or Grip-Tite 293 MSR cells Photinus pyralis

Protein Variants

Protein Variants Comment Organism
AC571TG nonsense mutation Photinus pyralis
additional information 10fold higher luminescence intensity of mutant enzyme luciferase FM compared to wild-type luciferase Photinus pyralis

Inhibitors

Inhibitors Comment Organism Structure
3,5-diphenyl-1,2,4-oxadiazole
-
Photinus pyralis
3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid decrease of Fluc proteolysis, 2 microM Photinus pyralis
3-[5-(2-methoxyphenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
3-[5-(3-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
3-[5-(3-methoxyphenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
3-[5-(4-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
3-[5-[2-(trifluoromethyl)phenyl]-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
3-[5-[4-(trifluoromethyl)phenyl]-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
4-methyl-N-(phenylmethyl)benzenesulfonamide
-
Photinus pyralis
4-[5-(2-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
4-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
4-[5-(3-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
4-[5-(3-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
geneticin induces nonsense suppression Photinus pyralis
gentamicin induces nonsense suppression Photinus pyralis
Limulus amebocyte lysate decreased luminescence intensity to 10% Photinus pyralis
N-(6-methoxy-1,3-benzothiazol-2-yl)-3-methylthiophene-2-carboxamide
-
Photinus pyralis
N-(6-methyl-1,3-benzothiazol-2-yl)thiophene-2-carboxamide
-
Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Photinus pyralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
41 relative luminescence units Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-aminoluciferin + ATP + O2 assay at 37°C. 5 min Photinus pyralis ?
-
?
D-luciferin + O2 10 microM, assay at pH 7.5 Photinus pyralis ?
-
?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
Photinus pyralis
fluc
-
Photinus pyralis
luciferase FM mutant luciferase Photinus pyralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Photinus pyralis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0014
-
4-[5-(2-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis
0.03
-
4-[5-(3-chlorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
-
Photinus pyralis