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Literature summary for 1.13.12.7 extracted from
- Identification and characterization of the Luc2-type luciferase in the Japanese firefly, Luciola parvula, involved in a dim luminescence in immobile stages (2017), Luminescence, 32, 924-931 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Luciola parvula |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00244 | - |
D-firefly luciferin | pH 8.0, 25°C | Luciola parvula |  |
| 0.018 | - |
D-firefly luciferin | pH 8.0, 25°C | Luciola parvula | |
| 0.123 | - |
ATP | pH 8.0, 25°C | Luciola parvula | |
| 0.428 | - |
ATP | pH 8.0, 25°C | Luciola parvula | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60100 | - |
calculated from sequence | Luciola parvula |
| 60353 | - |
calculated from sequence | Luciola parvula |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-firefly luciferin + O2 + ATP | Luciola parvula | - |
firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Luciola parvula | A0A146IHP8 | - |
- |
| Luciola parvula | H1AD95 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Luciola parvula |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Luciola parvula | - |
| larva | - |
Luciola parvula | - |
| larva | last instar larva | Luciola parvula | - |
| additional information | especially high expression in the adult male, no expression of LpLuc1 in eggs is detected under experimental conditions | Luciola parvula | - |
| additional information | no expression of LpLuc2 in the larva and adult male is detected under experimental conditions | Luciola parvula | - |
| pupa | - |
Luciola parvula | - |
| pupa | especially high expression in the pupa and adult female | Luciola parvula | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-firefly luciferin + O2 + ATP | - |
Luciola parvula | firefly oxyluciferin + CO2 + AMP + diphosphate + hv | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LpLuc1 | - |
Luciola parvula |
| LpLuc2 | - |
Luciola parvula |
| Luc1-type luciferase | - |
Luciola parvula |
| Luc2-type luciferase | - |
Luciola parvula |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Luciola parvula | calculated from sequence | - |
6.4 |
| Luciola parvula | calculated from sequence | - |
8.14 |
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