Literature summary for 1.13.12.7 extracted from

Wilkinson, I.V.L.; Reynolds, J.K.; Galan, S.R.G.; Vuorinen, A.; Sills, A.J.; Pires, E.; Wynne, G.M.; Wilson, F.X.; Russell, A.J.
Characterisation of utrophin modulator SMT C1100 as a non-competitive inhibitor of firefly luciferase (2020), Bioorg. Chem., 94, 103395 .

Search for more literature for 1.13.12.7

Application

Application	Comment	Organism
industry	the enzyme is widely used in academia and industry due to its excellent sensitivity and dynamic range, and its ease of use	Photinus pyralis
molecular biology	the enzyme is a powerful tool for molecular and cellular biology, and popular in high-throughput screening and drug discovery	Photinus pyralis

Inhibitors

Inhibitors	Comment	Organism	Structure
2,4-dimethoxy-N-(5-methylpyridin-2-yl)benzamide	non-competitive inhibitor	Photinus pyralis
2-(2-fluorophenyl)-6-methoxy-1,3-benzothiazole	luciferin competitor	Photinus pyralis
3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid	-	Photinus pyralis
5'-O-[[2-(6-hydroxy-1,3-benzothiazol-2-yl)-1,3-thiazole-4-carbonyl]sulfamoyl]adenosine	non-competitive inhibitor	Photinus pyralis
5-(ethanesulfonyl)-2-(naphthalen-2-yl)-1,3-benzoxazole	non-competitive inhibitor	Photinus pyralis
5-[(2E)-4-(4-bromophenyl)-4-oxobut-2-en-1-yl]-2-hydroxybenzoic acid	luciferin competitor	Photinus pyralis
dehydroluciferyl adenylate	-	Photinus pyralis
NFkappaBAI4	non-competitive inhibitor	Photinus pyralis
resveratrol	non-competitive inhibitor	Photinus pyralis
SMT C1100	non-competitive inhibitor, utrophin modulator, phase II clinical compound SMT C1100 for the treatment of Duchenne muscular dystrophy	Photinus pyralis
[2-(4-ethoxyphenyl)quinolin-4-yl][4-(pyridin-2-yl)piperazin-1-yl]methanone	ATP competitor	Photinus pyralis
[4-(6-methoxynaphthalen-2-yl)phenyl]acetic acid	luciferin competitor	Photinus pyralis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	Photinus pyralis	-	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Organism

Organism	UniProt	Comment	Textmining
Photinus pyralis	P08659	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	-	Photinus pyralis	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Synonyms

Synonyms	Comment	Organism
fluc	-	Photinus pyralis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000038	-	dehydroluciferyl adenylate	pH and temperature not specified in the publication	Photinus pyralis
0.00012	-	[4-(6-methoxynaphthalen-2-yl)phenyl]acetic acid	pH and temperature not specified in the publication	Photinus pyralis
0.00034	-	3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid	pH and temperature not specified in the publication	Photinus pyralis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000033	-	pH and temperature not specified in the publication	Photinus pyralis	5'-O-[[2-(6-hydroxy-1,3-benzothiazol-2-yl)-1,3-thiazole-4-carbonyl]sulfamoyl]adenosine
0.0002	-	pH and temperature not specified in the publication	Photinus pyralis	5-[(2E)-4-(4-bromophenyl)-4-oxobut-2-en-1-yl]-2-hydroxybenzoic acid
0.0003	-	pH and temperature not specified in the publication	Photinus pyralis	5-(ethanesulfonyl)-2-(naphthalen-2-yl)-1,3-benzoxazole
0.0005	-	pH and temperature not specified in the publication	Photinus pyralis	2-(2-fluorophenyl)-6-methoxy-1,3-benzothiazole
0.0005	-	pH and temperature not specified in the publication	Photinus pyralis	[2-(4-ethoxyphenyl)quinolin-4-yl][4-(pyridin-2-yl)piperazin-1-yl]methanone
0.001	-	pH and temperature not specified in the publication	Photinus pyralis	NFkappaBAI4
0.002	-	pH and temperature not specified in the publication	Photinus pyralis	resveratrol

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Release 2023.1 (January 2023)