Literature summary for 1.13.12.7 extracted from

Carvalho, M.C.; Tomazini, A.; Amaral, D.T.; Murakami, M.T.; Viviani, V.R.
Luciferase isozymes from the Brazilian Aspisoma lineatum (Lampyridae) firefly origin of efficient pH-sensitive lantern luciferases from fat body pH-insensitive ancestors (2020), Photochem. Photobiol. Sci., 19, 1750-1764 .

Search for more literature for 1.13.12.7

Activating Compound

Activating Compound	Comment	Organism	Structure
Li2+	-	Aspisoma lineatum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Aspisoma lineatum

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	-	Aspisoma lineatum
Cu2+	-	Aspisoma lineatum
Hg2+	-	Aspisoma lineatum
Ni2+	-	Aspisoma lineatum
Pb2+	-	Aspisoma lineatum
Zn2+	-	Aspisoma lineatum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	D-firefly luciferin	pH 8.0, temperature not specified in the publication, isoenzyme AL2	Aspisoma lineatum
0.004	-	ATP	pH 8.0, temperature not specified in the publication, isoenzyme AL1	Aspisoma lineatum
0.007	-	D-firefly luciferin	pH 8.0, temperature not specified in the publication, isoenzyme AL1	Aspisoma lineatum
0.007	-	ATP	pH 8.0, temperature not specified in the publication, isoenzyme AL2	Aspisoma lineatum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	Aspisoma lineatum	-	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspisoma lineatum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Aspisoma lineatum

Source Tissue

Source Tissue	Comment	Organism	Textmining
fat body	AL2 is the larval fat body isozyme	Aspisoma lineatum	-
lantern	adult lantern isozyme AL1	Aspisoma lineatum	-
larva	AL2 is the larval fat body isozyme	Aspisoma lineatum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	-	Aspisoma lineatum	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?
D-firefly luciferin + O2 + ATP	the larval fat body isozyme (AL2) displays very slow luminescence kinetics and a pH-insensitive spectrum. The adult lantern isozyme (AL1) displays flash-like kinetics and pH and metal sensitive bioluminescence spectra, and is at least 10 times catalytically more efficient than AL2	Aspisoma lineatum	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Synonyms

Synonyms	Comment	Organism
AL1	adult lantern isozyme	Aspisoma lineatum
AL2	larval fat body isozyme	Aspisoma lineatum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	half-life: 48 h, isoenzyme AL2. half-life: 1 h, isoenzyme AL1	Aspisoma lineatum
50	-	half-life: 3 h, isoenzyme AL2	Aspisoma lineatum
60	-	half-life: 10 min, isoenzyme AL2	Aspisoma lineatum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	isoenzyme AL1 and isoenzyme AL2	Aspisoma lineatum

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	pH 7.0: about 80% of maximal activity, pH 9.0: about 70% of maximal activity, isoenzyme AL1	Aspisoma lineatum
7.5	9.5	pH 7.5: about 50% of maximal activity, pH 9.5: about 50% of maximal activity, isoenzyme AL2	Aspisoma lineatum

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Release 2023.1 (January 2023)