Literature summary for 1.13.12.7 extracted from

  • Inouye, S.
    Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions (2010), Cell. Mol. Life Sci., 67, 387-404.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP essential for luminescence reaction Luciola cruciata
ATP essential for luminescence reaction Pyrophorus plagiophthalamus
molecular oxygen essential for luminescence reaction Photinus pyralis
molecular oxygen essential for luminescence reaction Luciola cruciata
molecular oxygen essential for luminescence reaction Pyrophorus plagiophthalamus
ATP essential for luminescence reaction, initiates luminescence reaction Photinus pyralis
CoA prevents rapid decay of light emission Photinus pyralis
diphosphate stimulates light production under some conditions Photinus pyralis
Triphosphate stimulates light production under some conditions Photinus pyralis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Photinus pyralis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Photinus pyralis

Engineering

Protein Variants Comment Organism
additional information chimeric protein with fatty acyl-CoA synthetase, EC 6.2.1.3, 4% of luminescence activity from wild-tpe luciferase activity Photinus pyralis
S286N red bioluminescence Luciola cruciata

Inhibitors

Inhibitors Comment Organism Structure
2-phenylbenzothiazole
-
Photinus pyralis
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine competitive inhibitor Photinus pyralis
AMP
-
Photinus pyralis
arachidonic acid concentration above 10 microM Photinus pyralis
ATP
-
Photinus pyralis
bromoform
-
Photinus pyralis
dehydroluciferin
-
Luciola cruciata
dehydroluciferin
-
Photinus pyralis
dehydroluciferyl adenylate
-
Luciola cruciata
dehydroluciferyl adenylate
-
Photinus pyralis
diphosphate depending on conditions Photinus pyralis
Halothane non-competitive inhibitor Photinus pyralis
linoleic acid concentration above 10 microM Photinus pyralis
lipoic acid
-
Photinus pyralis
L-luciferin
-
Luciola cruciata
L-luciferin
-
Photinus pyralis
myristic acid
-
Photinus pyralis
N-pyridin-2-ylbenzamide
-
Photinus pyralis
oleic acid concentration above 10 microM Photinus pyralis
oxyluciferin
-
Luciola cruciata
oxyluciferin
-
Photinus pyralis
pifithrin-alpha 20 microM, above 95% inhibition Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
ATP
-
Photinus pyralis
0.015
-
D-luciferin
-
Photinus pyralis
0.0024
-
D-luciferin native luciferase Photinus pyralis
0.0024
-
D-luciferin native luciferase Luciola cruciata
0.0047
-
D-luciferin recombinant luciferase Photinus pyralis
0.0047
-
D-luciferin recombinant luciferase Luciola cruciata
0.0136
-
linoleic acid
-
Photinus pyralis
0.0163
-
lauric acid
-
Luciola cruciata
0.00741
-
lauric acid
-
Photinus pyralis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essential Photinus pyralis
Mg2+ essential Luciola cruciata
Mg2+ essential Pyrophorus plagiophthalamus

Organism

Organism UniProt Comment Textmining
Pyrearinus termitilluminans AF116843
-
-
Photinus pyralis P08659
-
-
Luciola cruciata P13129
-
-
Aquatica lateralis Q01158
-
-
Lampyroidea maculata Q1WLP6
-
-
Luciola parvula Q25118
-
-
Pyrocoelia miyako Q26076
-
-
Luciola mingrelica Q26304
-
-
Lampyris noctiluca Q27688
-
-
Photuris pensylvanica Q27757
-
-
Lampyris turkestanicus Q5UFR2
-
-
Cratomorphus distinctus Q5USC8
-
-
Pyrophorus mellifluus Q717B6
-
-
Pyrophorus plagiophthalamus Q718F0
-
-
Hotaria unmunsana Q8T6U3
-
-
Pyrocoelia rufa Q9GPF9
-
-
Phrixothrix hirtus Q9U4U7
-
-
Phrixothrix vivianii Q9U4U8
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, affinity chromatography Photinus pyralis
ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC Luciola cruciata
ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC Aquatica lateralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
lower luminescence activity when another substrate than D-luciferin is used: only 10% luminescence activity with 6'-amino-D-luciferin, only 5% luminescence activity with 4'-methyl-D-luciferin, only 7% luminescence activity with D-quinolylluciferin, only 1.5% luminescence activity with D-naphthylluciferin, only 16% luminescence activity with D-5,5-dimethylluciferyl-adenylate Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauric acid + ATP + CoA
-
Photinus pyralis lauroyl-CoA + AMP + diphosphate
-
?
lauric acid + ATP + CoA
-
Luciola cruciata lauroyl-CoA + AMP + diphosphate
-
?
linoleic acid + ATP + CoA linoleic acid concentration above 10 microM has an inhibitory effect Photinus pyralis linoleoyl-CoA + AMP + diphosphate
-
?
4'-methyl-D-luciferin + ATP + O2
-
Photinus pyralis ?
-
?
5-methylluciferin + ATP + O2
-
Photinus pyralis ?
-
?
6'-amino-D-luciferin + ATP + O2
-
Photinus pyralis ?
-
?
D-5,5-dimethylluciferyl-adenylate + O2 + ATP red color of luminescence Pyrophorus plagiophthalamus ?
-
?
D-5,5-dimethylluciferyl-adenylate + O2 yellow-green color of luminescence Photinus pyralis ?
-
?
D-luciferin + ATP + O2
-
Luciola cruciata luciferyl-adenylate + diphosphate
-
r
D-luciferin + ATP + O2
-
Photinus pyralis luciferyl-adenylate + diphosphate + H2O + hv
-
r
D-naphthylluciferin + ATP + O2
-
Photinus pyralis ?
-
?
D-quinolylluciferin + ATP + O2
-
Photinus pyralis ?
-
?
dehydroluciferin + ATP
-
Photinus pyralis dehydroluciferyl-AMP + diphosphate
-
r
dehydroluciferin + ATP
-
Luciola cruciata dehydroluciferyl-AMP + diphosphate
-
r
luciferyl-adenylate + O2
-
Photinus pyralis oxyluciferin + AMP + CO2 + hv
-
ir
luciferyl-adenylate + O2
-
Luciola cruciata oxyluciferin + AMP + CO2 + hv
-
ir
R-COOH + ATP
-
Photinus pyralis R-CO-AMP + diphosphate
-
r
R-COOH + ATP
-
Luciola cruciata R-CO-AMP + diphosphate
-
r

Synonyms

Synonyms Comment Organism
luciferase
-
Cratomorphus distinctus
luciferase
-
Phrixothrix vivianii
luciferase
-
Phrixothrix hirtus
luciferase
-
Lampyris turkestanicus
luciferase
-
Luciola mingrelica
luciferase
-
Pyrophorus plagiophthalamus
luciferase
-
Aquatica lateralis
luciferase
-
Photuris pensylvanica
luciferase
-
Pyrocoelia miyako
luciferase
-
Pyrocoelia rufa
luciferase
-
Luciola parvula
luciferase
-
Hotaria unmunsana
luciferase
-
Lampyris noctiluca
luciferase
-
Lampyroidea maculata
luciferase
-
Pyrophorus mellifluus
luciferase
-
Pyrearinus termitilluminans
firefly luciferase
-
Photinus pyralis
firefly luciferase
-
Luciola cruciata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
yellow-green emitted light Photinus pyralis
5.4
-
red emitted light Photinus pyralis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
Photinus pyralis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Photinus pyralis
CoA
-
Photinus pyralis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
dehydroluciferyl adenylate value between 0.0000025 - 0.00125 mM Luciola cruciata
additional information
-
5'-O-[hydroxy({[(2Z)-2-(6-oxo-1,3-benzothiazol-2(6H)-ylidene)-2,3-dihydro-1,3-thiazol-4-yl]carbonyl}oxy)phosphoryl]adenosine value between 0.0000025-0.00125 mM Photinus pyralis
additional information
-
oxyluciferin value between 0.0005-0.002 mM Photinus pyralis
additional information
-
oxyluciferin value between 0.0005-0.002 mM Luciola cruciata
additional information
-
L-luciferin value between 0.003-0.004 mM Luciola cruciata
0.000041
-
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine for MgATP2- Photinus pyralis
0.00034
-
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine for luciferyl adenylate Photinus pyralis
0.000026
-
lipoic acid
-
Photinus pyralis
0.00053
-
myristic acid
-
Photinus pyralis
0.003 0.004 L-luciferin
-
Photinus pyralis
0.000034
-
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine for D-luciferin Photinus pyralis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000069
-
-
Photinus pyralis N-pyridin-2-ylbenzamide
0.00005
-
-
Photinus pyralis lipoic acid
0.00068
-
-
Photinus pyralis myristic acid

General Information

General Information Comment Organism
physiological function fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme Photinus pyralis
physiological function fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme Luciola cruciata