Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | essential for luminescence reaction | Luciola cruciata | |
ATP | essential for luminescence reaction | Pyrophorus plagiophthalamus | |
ATP | essential for luminescence reaction, initiates luminescence reaction | Photinus pyralis | |
CoA | prevents rapid decay of light emission | Photinus pyralis | |
diphosphate | stimulates light production under some conditions | Photinus pyralis | |
molecular oxygen | essential for luminescence reaction | Photinus pyralis | |
molecular oxygen | essential for luminescence reaction | Luciola cruciata | |
molecular oxygen | essential for luminescence reaction | Pyrophorus plagiophthalamus | |
Triphosphate | stimulates light production under some conditions | Photinus pyralis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Photinus pyralis |
Crystallization (Comment) | Organism |
---|---|
- |
Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chimeric protein with fatty acyl-CoA synthetase, EC 6.2.1.3, 4% of luminescence activity from wild-tpe luciferase activity | Photinus pyralis |
S286N | red bioluminescence | Luciola cruciata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-phenylbenzothiazole | - |
Photinus pyralis | |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | competitive inhibitor | Photinus pyralis | |
AMP | - |
Photinus pyralis | |
arachidonic acid | concentration above 10 microM | Photinus pyralis | |
ATP | - |
Photinus pyralis | |
bromoform | - |
Photinus pyralis | |
dehydroluciferin | - |
Luciola cruciata | |
dehydroluciferin | - |
Photinus pyralis | |
dehydroluciferyl adenylate | - |
Luciola cruciata | |
dehydroluciferyl adenylate | - |
Photinus pyralis | |
diphosphate | depending on conditions | Photinus pyralis | |
Halothane | non-competitive inhibitor | Photinus pyralis | |
L-luciferin | - |
Luciola cruciata | |
L-luciferin | - |
Photinus pyralis | |
linoleic acid | concentration above 10 microM | Photinus pyralis | |
lipoic acid | - |
Photinus pyralis | |
myristic acid | - |
Photinus pyralis | |
N-pyridin-2-ylbenzamide | - |
Photinus pyralis | |
oleic acid | concentration above 10 microM | Photinus pyralis | |
oxyluciferin | - |
Luciola cruciata | |
oxyluciferin | - |
Photinus pyralis | |
pifithrin-alpha | 20 microM, above 95% inhibition | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
D-luciferin | native luciferase | Photinus pyralis | |
0.0024 | - |
D-luciferin | native luciferase | Luciola cruciata | |
0.0047 | - |
D-luciferin | recombinant luciferase | Photinus pyralis | |
0.0047 | - |
D-luciferin | recombinant luciferase | Luciola cruciata | |
0.00741 | - |
lauric acid | - |
Photinus pyralis | |
0.0136 | - |
linoleic acid | - |
Photinus pyralis | |
0.015 | - |
D-luciferin | - |
Photinus pyralis | |
0.0163 | - |
lauric acid | - |
Luciola cruciata | |
0.16 | - |
ATP | - |
Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential | Photinus pyralis | |
Mg2+ | essential | Luciola cruciata | |
Mg2+ | essential | Pyrophorus plagiophthalamus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquatica lateralis | Q01158 | - |
- |
Cratomorphus distinctus | Q5USC8 | - |
- |
Hotaria unmunsana | Q8T6U3 | - |
- |
Lampyris noctiluca | Q27688 | - |
- |
Lampyris turkestanicus | Q5UFR2 | - |
- |
Lampyroidea maculata | Q1WLP6 | - |
- |
Luciola cruciata | P13129 | - |
- |
Luciola mingrelica | Q26304 | - |
- |
Luciola parvula | Q25118 | - |
- |
Photinus pyralis | P08659 | - |
- |
Photuris pensylvanica | Q27757 | - |
- |
Phrixothrix hirtus | Q9U4U7 | - |
- |
Phrixothrix vivianii | Q9U4U8 | - |
- |
Pyrearinus termitilluminans | AF116843 | - |
- |
Pyrocoelia miyako | Q26076 | - |
- |
Pyrocoelia rufa | Q9GPF9 | - |
- |
Pyrophorus mellifluus | Q717B6 | - |
- |
Pyrophorus plagiophthalamus | Q718F0 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC | Luciola cruciata |
ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC | Aquatica lateralis |
gel filtration, affinity chromatography | Photinus pyralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
lower luminescence activity when another substrate than D-luciferin is used: only 10% luminescence activity with 6'-amino-D-luciferin, only 5% luminescence activity with 4'-methyl-D-luciferin, only 7% luminescence activity with D-quinolylluciferin, only 1.5% luminescence activity with D-naphthylluciferin, only 16% luminescence activity with D-5,5-dimethylluciferyl-adenylate | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-methyl-D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
5-methylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
6'-amino-D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
D-5,5-dimethylluciferyl-adenylate + O2 | yellow-green color of luminescence | Photinus pyralis | ? | - |
? | |
D-5,5-dimethylluciferyl-adenylate + O2 + ATP | red color of luminescence | Pyrophorus plagiophthalamus | ? | - |
? | |
D-luciferin + ATP + O2 | - |
Luciola cruciata | luciferyl-adenylate + diphosphate | - |
r | |
D-luciferin + ATP + O2 | - |
Photinus pyralis | luciferyl-adenylate + diphosphate + H2O + hv | - |
r | |
D-naphthylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
D-quinolylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
dehydroluciferin + ATP | - |
Photinus pyralis | dehydroluciferyl-AMP + diphosphate | - |
r | |
dehydroluciferin + ATP | - |
Luciola cruciata | dehydroluciferyl-AMP + diphosphate | - |
r | |
lauric acid + ATP + CoA | - |
Photinus pyralis | lauroyl-CoA + AMP + diphosphate | - |
? | |
lauric acid + ATP + CoA | - |
Luciola cruciata | lauroyl-CoA + AMP + diphosphate | - |
? | |
linoleic acid + ATP + CoA | linoleic acid concentration above 10 microM has an inhibitory effect | Photinus pyralis | linoleoyl-CoA + AMP + diphosphate | - |
? | |
luciferyl-adenylate + O2 | - |
Photinus pyralis | oxyluciferin + AMP + CO2 + hv | - |
ir | |
luciferyl-adenylate + O2 | - |
Luciola cruciata | oxyluciferin + AMP + CO2 + hv | - |
ir | |
R-COOH + ATP | - |
Photinus pyralis | R-CO-AMP + diphosphate | - |
r | |
R-COOH + ATP | - |
Luciola cruciata | R-CO-AMP + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
firefly luciferase | - |
Luciola cruciata |
luciferase | - |
Cratomorphus distinctus |
luciferase | - |
Phrixothrix vivianii |
luciferase | - |
Phrixothrix hirtus |
luciferase | - |
Lampyris turkestanicus |
luciferase | - |
Luciola mingrelica |
luciferase | - |
Pyrophorus plagiophthalamus |
luciferase | - |
Aquatica lateralis |
luciferase | - |
Photuris pensylvanica |
luciferase | - |
Pyrocoelia miyako |
luciferase | - |
Pyrocoelia rufa |
luciferase | - |
Luciola parvula |
luciferase | - |
Hotaria unmunsana |
luciferase | - |
Lampyris noctiluca |
luciferase | - |
Lampyroidea maculata |
luciferase | - |
Pyrophorus mellifluus |
luciferase | - |
Pyrearinus termitilluminans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
red emitted light | Photinus pyralis |
7.6 | - |
yellow-green emitted light | Photinus pyralis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Photinus pyralis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Photinus pyralis | |
CoA | - |
Photinus pyralis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
dehydroluciferyl adenylate | value between 0.0000025 - 0.00125 mM | Luciola cruciata | |
additional information | - |
5'-O-[hydroxy({[(2Z)-2-(6-oxo-1,3-benzothiazol-2(6H)-ylidene)-2,3-dihydro-1,3-thiazol-4-yl]carbonyl}oxy)phosphoryl]adenosine | value between 0.0000025-0.00125 mM | Photinus pyralis | |
additional information | - |
oxyluciferin | value between 0.0005-0.002 mM | Photinus pyralis | |
additional information | - |
oxyluciferin | value between 0.0005-0.002 mM | Luciola cruciata | |
additional information | - |
L-luciferin | value between 0.003-0.004 mM | Luciola cruciata | |
0.000026 | - |
lipoic acid | - |
Photinus pyralis | |
0.000034 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for D-luciferin | Photinus pyralis | |
0.000041 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for MgATP2- | Photinus pyralis | |
0.00034 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for luciferyl adenylate | Photinus pyralis | |
0.00053 | - |
myristic acid | - |
Photinus pyralis | |
0.003 | 0.004 | L-luciferin | - |
Photinus pyralis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00005 | - |
- |
Photinus pyralis | lipoic acid | |
0.000069 | - |
- |
Photinus pyralis | N-pyridin-2-ylbenzamide | |
0.00068 | - |
- |
Photinus pyralis | myristic acid |
General Information | Comment | Organism |
---|---|---|
physiological function | fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme | Photinus pyralis |
physiological function | fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme | Luciola cruciata |