Literature summary for 1.13.12.7 extracted from

Rahban, M.; Salehi, N.; Saboury, A.A.; Hosseinkhani, S.; Karimi-Jafari, M.H.; Firouzi, R.; Rezaei-Ghaleh, N.; Moosavi-Movahedi, A.A.
Histidine substitution in the most flexible fragments of firefly luciferase modifies its thermal stability (2017), Arch. Biochem. Biophys., 629, 8-18 .

Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment)	Organism
-	Photinus pyralis

Protein Variants

Protein Variants	Comment	Organism
H461D	relative specific activity is 57.6% as compared to wild-type enzyme. Mutation decreases ATP binding affinity, reduces the melting temperature of protein by around 25°C and shifts its optimum temperature of activity to 10°C	Photinus pyralis
H489D	relative specific activity is 112% as compared to wild-type enzyme. Mutation introduces a new salt bridge between the C-terminal and N-terminal domains and increases protein rigidity but only slightly improves its thermal stability	Photinus pyralis
H489K	relative specific activity is 115% as compared to wild-type enzyme. Mutation increases protein rigidity but only slightly improves its thermal stability	Photinus pyralis
H489M	relative specific activity is 103% as compared to wild-type enzyme	Photinus pyralis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	-	D-firefly luciferin	pH 7.8, temperature not specified in the publication, mutant enzyme H489K	Photinus pyralis
0.0023	-	D-firefly luciferin	pH 7.8, temperature not specified in the publication, mutant enzyme H489M	Photinus pyralis
0.0025	-	D-firefly luciferin	pH 7.8, temperature not specified in the publication, mutant enzyme H489D	Photinus pyralis
0.005	-	D-firefly luciferin	pH 7.8, temperature not specified in the publication, mutant enzyme H461D	Photinus pyralis
0.005	-	D-firefly luciferin	pH 7.8, temperature not specified in the publication, wild-type enzyme	Photinus pyralis
0.04	-	ATP	pH 7.8, temperature not specified in the publication, mutant enzyme H489M	Photinus pyralis
0.06	-	ATP	pH 7.8, temperature not specified in the publication, mutant enzyme H489K	Photinus pyralis
0.06	-	ATP	pH 7.8, temperature not specified in the publication, wild-type enzyme	Photinus pyralis
0.09	-	ATP	pH 7.8, temperature not specified in the publication, mutant enzyme H489D	Photinus pyralis
0.1	-	ATP	pH 7.8, temperature not specified in the publication, mutant enzyme H461D	Photinus pyralis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	Photinus pyralis	-	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Organism

Organism	UniProt	Comment	Textmining
Photinus pyralis	P08659	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Photinus pyralis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-firefly luciferin + O2 + ATP	-	Photinus pyralis	firefly oxyluciferin + CO2 + AMP + diphosphate + hv	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
10	-	mutant enzyme H461D	Photinus pyralis
20	-	mutant enzyme H489M	Photinus pyralis
25	-	wild-type enzyme	Photinus pyralis
25	-	mutant enzyme H489D	Photinus pyralis
30	-	mutant enzyme H489K	Photinus pyralis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	t1/2: 17 min (wild-type enzyme), 9.5 min (mutant enzyme H461D), 12.6 min (mutant enzyme H489K), 20.2 min (mutant enzyme H489D) and 24.4 min (mutant enzyme H489M)	Photinus pyralis
30	-	t1/2: 10.5 min (wild-type enzyme), 6.9 min (mutant enzyme H461D), 8.3 min (mutant enzyme H489K), 11.1 min (mutant enzyme H489D) and 9.5 min (mutant enzyme H489M)	Photinus pyralis
35	-	t1/2: 6.1 min (wild-type enzyme), 3.7 min (mutant enzyme H461D), 6.4 min (mutant enzyme H489K), 6.5 min (mutant enzyme H489D) and 5.7 min (mutant enzyme H489M)	Photinus pyralis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	wild-type enzyme, mutant enzymes H461D, H489K, H489D and H489M	Photinus pyralis

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Release 2023.1 (January 2023)