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Literature summary for 1.13.12.7 extracted from

  • Law, G.H.; Gandelman, O.A.; Tisi, L.C.; Lowe, C.R.; Murray, J.A.
    Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance (2006), Biochem. J., 397, 305-312.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Photinus pyralis

Protein Variants

Protein Variants Comment Organism
F14R improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
F14R/L35Q/V182K/I232K/F465R improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
F465R improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
I232K improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
L35Q improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis
V182K improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetate agarose column chromatography Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Photinus luciferin + O2 + ATP
-
Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

Synonyms

Synonyms Comment Organism
Photinus pyralis luciferase
-
Photinus pyralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
Photinus pyralis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 45
-
Photinus pyralis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
mutant enzymes F14R, L35Q, V182K, I232K and F465R are stable up to 45°C Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Photinus pyralis

pI Value

Organism Comment pI Value Maximum pI Value
Photinus pyralis His10-tagged wild type enzyme
-
7.2