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Literature summary for 1.13.12.7 extracted from

  • Fushimi, T.; Miura, N.; Shintani, H.; Tsunoda, H.; Kuroda, K.; Ueda, M.
    Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering (2013), Appl. Microbiol. Biotechnol., 97, 4003-4011.
    View publication on PubMed
Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Photinus pyralis

Protein Variants

Protein Variants Comment Organism
S440P/S456Y mutant shows improved specificity and reactivity for ATP compared to wild-type Photinus pyralis
S440R/S456A mutant shows improved specificity and reactivity for ATP compared to wild-type Photinus pyralis
S440R/S456V mutant shows improved specificity for ATP compared to wild-type Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + ATP + O2
-
 Photinus pyralis oxidized D-luciferin + AMP + CO2 + diphosphate
-
 ?
D-luciferin + dATP + O2
-
 Photinus pyralis ?
-
 ?

Synonyms

Synonyms Comment Organism
luciferase
-
 Photinus pyralis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Photinus pyralis