Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.12.7 extracted from

  • Fujii, H.; Noda, K.; Asami, Y.; Kuroda, A.; Sakata, M.; Tokida, A.
    Increase in bioluminescence intensity of firefly luciferase using genetic modification (2007), Anal. Biochem., 366, 131-136.
    View publication on PubMed
Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain HMS174(DE3) Photinus pyralis

Protein Variants

Protein Variants Comment Organism
D436A 3.4fold increased activity compared to the wild type enzyme Photinus pyralis
D436E 50% activity compared to the wild type enzyme Photinus pyralis
D436G 8.5fold increased activity compared to the wild type enzyme Photinus pyralis
D436N 50% activity compared to the wild type enzyme Photinus pyralis
D436S 2.2fold increased activity compared to the wild type enzyme Photinus pyralis
D436V 2% activity compared to the wild type enzyme Photinus pyralis
I147A 30% activity compared to the wild type enzyme Photinus pyralis
I147E 4% activity compared to the wild type enzyme Photinus pyralis
I147F 1.2fold increased activity compared to the wild type enzyme Photinus pyralis
I147G 3% activity compared to the wild type enzyme Photinus pyralis
I147K 4% activity compared to the wild type enzyme Photinus pyralis
I147L 4.7fold increased activity compared to the wild type enzyme Photinus pyralis
I147M 3.4fold increased activity compared to the wild type enzyme Photinus pyralis
I147Q 70% activity compared to the wild type enzyme Photinus pyralis
I147R 1% activity compared to the wild type enzyme Photinus pyralis
I147S 7% activity compared to the wild type enzyme Photinus pyralis
I147V 1.3fold increased activity compared to the wild type enzyme Photinus pyralis
L530A 20% activity compared to the wild type enzyme Photinus pyralis
L530D 1% activity compared to the wild type enzyme Photinus pyralis
L530F 10% activity compared to the wild type enzyme Photinus pyralis
L530H 1.4fold increased activity compared to the wild type enzyme Photinus pyralis
L530I shows wild type enzyme activity Photinus pyralis
L530K 3.2fold increased activity compared to the wild type enzyme Photinus pyralis
L530R 5.1fold increased activity compared to the wild type enzyme Photinus pyralis
L530S 1% activity compared to the wild type enzyme Photinus pyralis
L530V 50% activity compared to the wild type enzyme Photinus pyralis
L530Y 2% activity compared to the wild type enzyme Photinus pyralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 D-luciferin mutant enzyme D436G, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis
0.0037
-
 D-luciferin mutant enzyme L530R, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis
0.0043
-
 D-luciferin mutant enzyme I432L, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis
0.009
-
 ATP mutant enzyme D436G, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis
0.0211
-
 D-luciferin wild type enzyme, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis
0.0456
-
 ATP mutant enzyme I432L, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis
0.0538
-
 ATP mutant enzyme L530R, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis
0.1602
-
 ATP wild type enzyme, in 50 mM Tris-HCl, pH 7.4, and 10 mM MgCl2 Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis Q27758
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose 6 Fast Flow column chromatography Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + O2 + ATP
-
 Photinus pyralis oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
 Photinus pyralis

pI Value

Organism Comment pI Value Maximum pI Value
Photinus pyralis isoelectric focusing
-
 6